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The analysis of cell-type specific cellular responses under different irradiation conditions and dynamic regulation of p53 functions

Research Project

Project/Area Number 23710069
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Risk sciences of radiation/Chemicals
Research InstitutionHiroshima University

Principal Investigator

KAWAI Hidehiko  広島大学, 原爆放射線医科学研究所, 助教 (30379846)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywords応答 / p53 / 照射線量率 / iPS細胞 / 放射線影響 / ゲノム安定性維持機構 / 放射線 / 細胞応答 / ゲノム安定性
Research Abstract

Various types of human cultured cells, including induced pluripotent stem cells (hiPSCs), were cultured under chronic gamma-irradiation at low and middle dose-rates (10-1000 mGy/day). The cell-type specific cellular responses of these cells to irradiation and the underlying molecular mechanisms were analyzed to examine their dose-rate-dependence. A combination of chronic irradiation culture conditions and advanced high-throughput techniques, such as the next generation sequencing system and an automated image analyzing system, could reveal a variety of cellular systems for the maintenance of the genomic integrity and the molecular mechanisms of p53-dependent cellular responses.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (20 results)

All 2013 2012 2011 Other

All Journal Article (10 results) (of which Peer Reviewed: 10 results) Presentation (10 results)

  • [Journal Article] Aberrant microRNA expression in radiation-induced rat mammary cancer: the potential role of miR-194 overexpression in cancer cell proliferation2013

    • Author(s)
      Iizuka, D., Imaoka, T., Nishimura, M., Kawai, H., Suzuki, F., Shimada, Y.
    • Journal Title

      Radiat Res.

      Volume: 179(2) Pages: 151-159

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Aberrant microRNA expression in radiation-induced rat mammary cancer: the potential role of miR-194 overexpression in cancer cell proliferation.2013

    • Author(s)
      Iizuka D, Imaoka T, Nishimura M, Kawai H, Suzuki F, Shimada Y.
    • Journal Title

      Radiat Res

      Volume: 179(2) Pages: 151-9

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] En bloc transfer of polyubiquitin chains to PCNA in vitro is mediated by two different human E2-E3 pairs2012

    • Author(s)
      Masuda, Y., Suzuki, M., Kawai, H., Hishiki, A., Hashimoto, H., Masutani, C., Hishida, T., Suzuki, F., Kamiya, K
    • Journal Title

      Nucleic Acids Res

      Volume: 40(20) Pages: 10394-10407

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Aurora kinase-A inactivates DNA damage-induced apoptosis and spindle assembly checkpoint response functions of p732012

    • Author(s)
      Katayama, H., Wang, J., Treekitkarnmongkol, W., Kawai, H., Sasai, K., Zhang, H., Wang, H., Adams, HP., Jiang, S., Chakraborty, SN., Suzuki, F., Arlinghaus, RB., Liu, J., Mobley, JA., Grizzle, WE., Wang, H., Sen, S.
    • Journal Title

      Cancer Cell

      Volume: 21(2) Pages: 196-211

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Asymmetric nature of two subunits of RAD18, a RING-type ubiquitin ligase E3, in the human RAD6A-RAD18 ternary complex2012

    • Author(s)
      Masuda, Y., Suzuki, M., Kawai, H., Suzuki, F., Kamiya, K.
    • Journal Title

      Nucleic Acids Res.

      Volume: 40(3) Pages: 1065-1076

    • NAID

      120005147856

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] En bloc transfer of polyubiquitin chains to PCNA in vitro is mediated by two different human E2-E3 pairs.2012

    • Author(s)
      Masuda Y, Suzuki M, Kawai H, Hishiki A, Hashimoto H, Masutani C, Hishida T, Suzuki F, Kamiya K.
    • Journal Title

      Nucleic Acids Res

      Volume: 40(20) Pages: 10394-407

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Asymmetric nature of two subunits of RAD18, a RING-type ubiquitin ligase E3, in the human RAD6A-RAD18 ternary complex.2012

    • Author(s)
      Masuda, Y., Suzuki, M., Kawai, H., Suzuki, F., and Kamiya, K.
    • Journal Title

      Nucleic Acids Res.

      Volume: 40(3) Pages: 1065-1076

    • NAID

      120005147856

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Aurora Kinase-A Inactivates DNA Damage-Induced Apoptosis and Spindle Assembly Checkpoint Response Functions of p73.2012

    • Author(s)
      Katayama, H., Wang, J., Treekitkarnmongkol, W., Kawai, H., et al.
    • Journal Title

      Cancer Cell.

      Volume: 21(2) Pages: 196-211

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] The p53 inhibitors MDM2/MDMX complex is required for control of p53 activity in vivo2011

    • Author(s)
      Huang, L., Yan, Z., Liao, X., Li, Y., Yang, J., Wang, ZG., Zuo, Y., Kawai, H., Shadfan, M., Ganapathy, S., Yuan, ZM
    • Journal Title

      Proc. Natl. Acad. Sci. U. S. A.

      Volume: 108(29) Pages: 12001-12006

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] The p53 inhibitors MDM2/MDMX complex is required for control of p53 activity in vivo.2011

    • Author(s)
      Huang, L., Yan, Z., Liao, X., Li, Y., Yang, J., Wang, ZG., Zuo, Y., Kawai, H., Shadfan, M., Ganapathy, S., and Yuan, ZM.
    • Journal Title

      Proc. Natl. Acad. Sci. U. S. A.

      Volume: 108(29) Pages: 12001-12006

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] Comprehensive analysis of cellular responses to chronic γ-irradiation2013

    • Author(s)
      河合秀彦
    • Organizer
      3rd International Symposium of RIRBM
    • Place of Presentation
      広島市
    • Year and Date
      2013-02-13
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Presentation] The role of p53 in human iPS cells in the cellular response to DNA-damage2012

    • Author(s)
      河合秀彦
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡市
    • Year and Date
      2012-12-13
    • Related Report
      2012 Final Research Report
  • [Presentation] 異なる線量率のγ線照射下での 細胞応答の多次元解析2012

    • Author(s)
      河合秀彦
    • Organizer
      日本放射線影響学会第55回大会
    • Place of Presentation
      仙台市
    • Year and Date
      2012-09-08
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Presentation] 慢性的な放射線照射に対する細胞応答機構の解析2012

    • Author(s)
      河合秀彦
    • Organizer
      第37回中国地区放射線影響研究会
    • Place of Presentation
      広島市
    • Year and Date
      2012-07-27
    • Related Report
      2012 Final Research Report
  • [Presentation] γ線の照射環境下での細胞応答の線量率依存性の解析2011

    • Author(s)
      河合秀彦
    • Organizer
      日本放射線影響学会第54回大会
    • Place of Presentation
      神戸市
    • Year and Date
      2011-11-19
    • Related Report
      2012 Final Research Report
  • [Presentation] 恒常的な放射線照射に対する細胞応答の線量率依存性の解析2011

    • Author(s)
      河合秀彦
    • Organizer
      第52回原子爆弾後障害研究会
    • Place of Presentation
      広島市
    • Year and Date
      2011-06-05
    • Related Report
      2012 Final Research Report
  • [Presentation] 恒常的な放射線照射に対する細胞応答の線量率依存性の解析2011

    • Author(s)
      河合秀彦
    • Organizer
      第52回原子爆弾後障害研究会
    • Place of Presentation
      広島
    • Related Report
      2011 Research-status Report
  • [Presentation] γ線の照射環境下での細胞応答の線量率依存性の解析2011

    • Author(s)
      河合秀彦
    • Organizer
      日本放射線影響学会第54回大会
    • Place of Presentation
      神戸
    • Related Report
      2011 Research-status Report
  • [Presentation] 慢性的な放射線照射に対する細胞応答機構の解析

    • Author(s)
      河合秀彦
    • Organizer
      第37回中国地区放射線影響研究会
    • Place of Presentation
      広島市
    • Related Report
      2012 Annual Research Report
  • [Presentation] The role of p53 in human iPS cells in the cellular response to DNA-damage

    • Author(s)
      河合秀彦
    • Organizer
      第35回日本分子生物学会年回
    • Place of Presentation
      福岡市
    • Related Report
      2012 Annual Research Report

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Published: 2011-08-05   Modified: 2019-07-29  

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