The analysis of cell-type specific cellular responses under different irradiation conditions and dynamic regulation of p53 functions
| Project/Area Number |
23710069
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Risk sciences of radiation/Chemicals
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| Research Institution | Hiroshima University |
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Principal Investigator |
KAWAI Hidehiko 広島大学, 原爆放射線医科学研究所, 助教 (30379846)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | 応答 / p53 / 照射線量率 / iPS細胞 / 放射線影響 / ゲノム安定性維持機構 / 放射線 / 細胞応答 / ゲノム安定性 |
|---|
| Research Abstract |
Various types of human cultured cells, including induced pluripotent stem cells (hiPSCs), were cultured under chronic gamma-irradiation at low and middle dose-rates (10-1000 mGy/day). The cell-type specific cellular responses of these cells to irradiation and the underlying molecular mechanisms were analyzed to examine their dose-rate-dependence. A combination of chronic irradiation culture conditions and advanced high-throughput techniques, such as the next generation sequencing system and an automated image analyzing system, could reveal a variety of cellular systems for the maintenance of the genomic integrity and the molecular mechanisms of p53-dependent cellular responses.
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Report
(3 results)
Research Products
(20 results)
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[Journal Article] Aberrant microRNA expression in radiation-induced rat mammary cancer: the potential role of miR-194 overexpression in cancer cell proliferation2013
Author(s)
Iizuka, D., Imaoka, T., Nishimura, M., Kawai, H., Suzuki, F., Shimada, Y.
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Journal Title
Radiat Res.
Volume: 179(2)
Pages: 151-159
Related Report
Peer Reviewed
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[Journal Article] En bloc transfer of polyubiquitin chains to PCNA in vitro is mediated by two different human E2-E3 pairs2012
Author(s)
Masuda, Y., Suzuki, M., Kawai, H., Hishiki, A., Hashimoto, H., Masutani, C., Hishida, T., Suzuki, F., Kamiya, K
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Journal Title
Nucleic Acids Res
Volume: 40(20)
Pages: 10394-10407
Related Report
Peer Reviewed
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[Journal Article] Aurora kinase-A inactivates DNA damage-induced apoptosis and spindle assembly checkpoint response functions of p732012
Author(s)
Katayama, H., Wang, J., Treekitkarnmongkol, W., Kawai, H., Sasai, K., Zhang, H., Wang, H., Adams, HP., Jiang, S., Chakraborty, SN., Suzuki, F., Arlinghaus, RB., Liu, J., Mobley, JA., Grizzle, WE., Wang, H., Sen, S.
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Journal Title
Cancer Cell
Volume: 21(2)
Pages: 196-211
Related Report
Peer Reviewed
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[Journal Article] Asymmetric nature of two subunits of RAD18, a RING-type ubiquitin ligase E3, in the human RAD6A-RAD18 ternary complex2012
Author(s)
Masuda, Y., Suzuki, M., Kawai, H., Suzuki, F., Kamiya, K.
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Journal Title
Nucleic Acids Res.
Volume: 40(3)
Pages: 1065-1076
NAID
Related Report
Peer Reviewed
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[Journal Article] Asymmetric nature of two subunits of RAD18, a RING-type ubiquitin ligase E3, in the human RAD6A-RAD18 ternary complex.2012
Author(s)
Masuda, Y., Suzuki, M., Kawai, H., Suzuki, F., and Kamiya, K.
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Journal Title
Nucleic Acids Res.
Volume: 40(3)
Pages: 1065-1076
NAID
Related Report
Peer Reviewed
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[Journal Article] The p53 inhibitors MDM2/MDMX complex is required for control of p53 activity in vivo2011
Author(s)
Huang, L., Yan, Z., Liao, X., Li, Y., Yang, J., Wang, ZG., Zuo, Y., Kawai, H., Shadfan, M., Ganapathy, S., Yuan, ZM
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Journal Title
Proc. Natl. Acad. Sci. U. S. A.
Volume: 108(29)
Pages: 12001-12006
Related Report
Peer Reviewed
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[Journal Article] The p53 inhibitors MDM2/MDMX complex is required for control of p53 activity in vivo.2011
Author(s)
Huang, L., Yan, Z., Liao, X., Li, Y., Yang, J., Wang, ZG., Zuo, Y., Kawai, H., Shadfan, M., Ganapathy, S., and Yuan, ZM.
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Journal Title
Proc. Natl. Acad. Sci. U. S. A.
Volume: 108(29)
Pages: 12001-12006
Related Report
Peer Reviewed
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