| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Research Abstract |
In this study, I comprehensively analyzed the importance of microRNA (miRNA) on radiation-induced mammary carcinogenesis. The inhibition of miR-194 suppressed the cell growth rate in some human breast cancer cell lines. I obtained breast cancer stem cells under specific culture condition (mammosphere culture). In these cells, the upregulation of miR-22 was observed. The manipulation of miR-22 level changed not only the frequency of mammosphere formation but also the sensitivity to radiation exposure evaluated by colony formation assay. The upregulation of miR-194 in sera of rat radiation-induced mammary carcinoma model was observed in part but not significant.
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