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A study on genotoxicity of nanomaterials using mammalian cultured cells

Research Project

Project/Area Number 23710081
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Risk sciences of radiation/Chemicals
Research InstitutionOsaka Prefecture University

Principal Investigator

KAWANISHI Masanobu  大阪府立大学, 大阪府立大学, 助教 (70332963)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywordsナノマテリアル / ナノマグネタイト / 遺伝毒性 / 培養細胞 / 小核試験 / 姉妹染色分体交換試験 / DNA二重鎖切断 / 活性酸素種 / 環境質定量化・予測 / ナノ材料
Research Abstract

We examined the genotoxicity of magnetite nanoparticles (primary particle size: 10 nm) on human A549 and Chinese hamster ovary (CHO) AA8 cells. Six hours’ treatment with the particles dose-dependently increased the frequency of micronuclei (MN) in the A549 and CHO AA8 cells up to 5.2% and 5.0% at a dose of 200 μg/mL (34 μg/cm2), respectively. In A549 cells, treatment with the nanoparticles (2 μg/mL) for 1 hour induced H2AX phosphorylation, which is suggestive of DNA double strand breaks (DSB). Treating CHO AA8 cells with 2 μg/mL magnetite for 1 hour resulted in a five times higher frequency of sister chromatid exchange (SCE) than the control level. We detected reactive oxygen species (ROS) in CHO cells treated with the particles. These findings indicate that magnetite nanoparticles induce ROS in mammalian cells, leading to the direct or indirect induction of DSB, followed by clastogenic events including MN and SCE.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (11 results)

All 2013 2012 2011

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (7 results)

  • [Journal Article] Genotoxicity and reactive oxygenspecies production induced by magnetitenanoparticles in mammalian cells2013

    • Author(s)
      Masanobu Kawanishi, Sayaka Ogo, MihoIkemoto, Yukari Totsuka, Kousuke Ishino,Keiji Wakabayashic Takashi Yagi
    • Journal Title

      Journalof Toxicological Sciences

      Volume: 38(3)

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Genotoxicity and reactive oxygen species production induced by magnetite nanoparticles in mammalian cells2013

    • Author(s)
      Masanobu Kawanishi, et al.
    • Journal Title

      Journal of Toxicological Sciences

      Volume: accepted Pages: 1-1

    • NAID

      10031158916

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genotoxicity of multi-walled carbon nanotubes in both in vitro and in vivo assay systems2012

    • Author(s)
      Kato, T., et al
    • Journal Title

      Nanotoxicology

      Volume: (in press) Issue: 4 Pages: 452-461

    • DOI

      10.3109/17435390.2012.674571

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] In Vitro and In Vivo Genotoxicity Induced by Fullerene (C60) and Kaolin.2011

    • Author(s)
      Yukari Totsuka, Tatsuya Kato, Shu-ichi Masuda, Kousuke Ishino, Yoko Matsumoto, Sumio Goto, Masanobu Kawanishi, Takashi Yagi and Keiji Wakabayashi.
    • Journal Title

      Genes and Environment

      Volume: 33 Pages: 14-20

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] カオリンの遺伝毒性発現メカニズムの解明2012

    • Author(s)
      池本実穂, 川西優喜, 戸塚ゆ加里,若林敬二, 八木孝司
    • Organizer
      日本環境変異原学会第41回大会
    • Place of Presentation
      静岡
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Presentation] Genotoxicity of nanomaterialsmediated by ROS generation in vitro2012

    • Author(s)
      MihoIkemot, Masanobu Kawanishi, YukariTotsuka, Keiji Wakabayashi, Takashi Yagi
    • Organizer
      3rd Asian Conference of EnvironmentalMutagens
    • Place of Presentation
      China
    • Related Report
      2012 Final Research Report
  • [Presentation] Genotoxicity of nanomaterials mediated by ROS generation in vitro2012

    • Author(s)
      Miho Ikemot, Masanobu Kawanishi, Yukari Totsuka, Keiji Wakabayashi, Takashi Yagi
    • Organizer
      3rd Asian Conference of Environmental Mutagens
    • Place of Presentation
      China
    • Related Report
      2012 Annual Research Report
  • [Presentation] Genotoxicity induced by nanoparticles2011

    • Author(s)
      Yukari Totsuka, Tatsuya Kato, KousukeIshino, Shuichi Masuda, Dai Nakae, YukieTada, Masanobu Kawanishi, Takashi Yagi,Masatoshi Watanabe, Keiji Wakabayashi,Hitoshi Nakagama
    • Organizer
      41st European Environmental Mutagen Society annualconference
    • Place of Presentation
      Barcelona, Spain
    • Related Report
      2012 Final Research Report
  • [Presentation] ナノ材料による小核誘導における相同組換え修復の関与2011

    • Author(s)
      池本実穂, 川西優喜,戸塚ゆ加里, 若林敬二, 八木孝司
    • Organizer
      日本環境変異原学会第40回大会
    • Place of Presentation
      東京
    • Related Report
      2012 Final Research Report
  • [Presentation] GENOTOXICITY INDUCED BY NANOPARTICLES2011

    • Author(s)
      Yukari Totsuka, Tatsuya Kato, Kousuke Ishino, Shuichi Masuda, Dai Nakae, Yukie Tada, Masanobu Kawanishi, Takashi Yagi, Masatoshi Watanabe, Keiji Wakabayashi, Hitoshi Nakagama
    • Organizer
      European Environmental Mutagen Society
    • Place of Presentation
      バルセロナ、スペイン
    • Related Report
      2011 Research-status Report
  • [Presentation] ナノ材料による小核誘導における相同組換え修復の関与2011

    • Author(s)
      池本実穂、川西優喜、戸塚ゆ加里、若林敬二、八木 孝司
    • Organizer
      日本環境変異原学会
    • Place of Presentation
      東京
    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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