Formation of DNA adducts and mechanism of mutagenesis induced by nanomaterials
| Project/Area Number |
23710084
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Risk sciences of radiation/Chemicals
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| Research Institution | Nippon Medical School (2013)
National Center of Neurology and Psychiatry (2011-2012) |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | DNA付加体 / 突然変異 / ナノマテリアル / 酸化ストレス / LC-MS/MS / アダクトミクス / マグネタイト / アダクトーム解析 |
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| Research Abstract |
Nanomaterials are useful owing to their characteristic properties and are commonly used in various fields. Nanosized magnetite (MGT) is widely utilized in medicinal and industrial fields; however, their toxicological properties are not well studied. In our previous study, we found that DNA damage analyzed by the comet assay of the lungs of ICR mice intratracheally instilled with a single dose of MGT at 0.05 or 0.2 mg/animal was approximately two- to three-fold greater than that of vehicle-instilled ICR mice. In this study, a comprehensive DNA adduct analysis (DNA adductome) revealed that a broad array of DNA adducts, including etheno-dA and heptanone-etheno-dC, were produced and that most of the DNA adducts determined as the major contributors in the MGT-exposed group could be derived from inflammation. These findings suggest that inflammatory responses are probably involved in the genotoxicity induced by MGT in the lungs of mice.
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Report
(4 results)
Research Products
(14 results)
