| Project/Area Number |
23710096
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Environmental technology/Environmental materials
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| Research Institution | Akita Prefectural University |
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Principal Investigator |
OKANO Kunihiro 秋田県立大学, 生物資源科学部, 助教 (30455927)
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| Research Collaborator |
SUGIURA Norio 筑波大学, 大学院・生命環境科学研究科, 教授 (10302374)
FUKUSHIMA Jun 秋田県立大学, 生物資源科学部, 准教授 (00181256)
ASANO Ryoki 秋田県立大学, 生物資源科学部, 流動研究員 (20646137)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
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| Keywords | アオコ / microcystin / 八郎湖 / 分子生物学的手法 / 藍藻類 / 遺伝子 / 環境分析 / PCR / 有毒アオコ |
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| Research Abstract |
With the ultimate goal of clarifying the algal bloom outbreak mechanism in Lake Hachiro-ko (Akita Prefecture), a novel method was developed for determining quantity of toxic and non-toxic cyanobacteria, by combining competitive PCR with a D NA/RNA analytical microchip electrophoresis device. In addition, the comprehensive genetic diversity analysis of the algal bloom outbreak waters with particular focus attention on eubacteria including cyanobacteria was carried out using a next generation sequencer (NGS). The results confirmed the succession of bacterial populations predicted to contribute to the degradation of sheath (extracellular polysaccharide of cyanobacteria) and the cyanotoxin, microcystin.
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