| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
|
|---|
| Research Abstract |
Acetoacetyl-CoA reductase (PhaB) catalyzes the reduction of acetoacetyl-CoA into 3-hydroxubutyryl-CoA using NADPH as a reducing power.3-Hydroxybutyryl-CoA serves as a monomer substrate for the microbial polyester, polyhydroxybutyrate. The aim of this study is to engineer PhaB for enhancing the enzymatic activity. The kinetic analysis of the selected mutants revealed that they possess enhanced turnover rate. The effectiveness of the obtained mutants of PhaB on polyhydroxybutyrate production was evaluated using Corynebacterium glutamicum, indicating that the content of polyhydroxybutyrate was increased up to 3-fold compared to the wild-type PhaB.
|
