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Investigation of Nanospace at Biological Membrane/Biological Solution Interfaces by Three-Dimensional Scanning Force Microscopy

Research Project

Project/Area Number 23710142
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Nanomaterials/Nanobioscience
Research InstitutionKanazawa University

Principal Investigator

ASAKAWA Hitoshi  金沢大学, バイオAFM先端研究センター, 助教 (90509605)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords原子間力顕微鏡 / 脂質二重膜 / 生体膜 / 水和構造 / ナノ計測 / ナノバイオサイエンス / 脂質膜
Research Abstract

In this project, I have developed a closed fluid cell with liquid-sealing mechanism to improve the stability and the reproducibility of three-dimensional scanning force microscopy (3D-SFM). The results showed the applicability of the developed closed fluid cell to atomic-scale AFM experiments. In addition, I demonstrated the 3D visualization of hydration structures and lipid headgroups at the interface between DPPC bilayer and water by 3D-SFM. The results suggest the applicability of 3D-SFM imaging to molecular-scale studies of biological membrane/biological solution interfaces.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (24 results)

All 2014 2013 2012 2011 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (18 results) (of which Invited: 3 results) Remarks (1 results) Patent(Industrial Property Rights) (2 results) (of which Overseas: 1 results)

  • [Journal Article] Closed Fluid Cell with Liquid-Sealing Mechanism for Stable and Flexible Operation of Liquid-Environment Atomic Force Microscopy2013

    • Author(s)
      H. Asakawa, Y. Katagiri, T. Fukuma
    • Journal Title

      Jpn. J. Appl. Phys.

      Volume: 52 Issue: 11R Pages: 110109-110109

    • DOI

      10.7567/jjap.52.110109

    • NAID

      210000143030

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Three-dimensional quantitative force maps in liquid with 10 piconewton, angstrom and sub-minute resolutions2013

    • Author(s)
      E. T. Herruzo, H. Asakawa, T. Fukuma, R. Garcia
    • Journal Title

      Nanoscale

      Volume: 5 Issue: 7 Pages: 2678-2685

    • DOI

      10.1039/c2nr33051b

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Spatial Distribution of Lipid Headgroups and Water Molecules at membrane/Water Interfaces Visualized by Three-Dimensional Scanning Force Microscopy2012

    • Author(s)
      H.Asakawa, S. Yoshioka, K. Nishimura, T. Fukuma
    • Journal Title

      ACS Nano

      Volume: 6 Issue: 10 Pages: 9013-9020

    • DOI

      10.1021/nn303229j

    • NAID

      120004997010

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Presentation] 3次元走査型力顕微鏡による多層状脂質二重膜のナノスケール計測2014

    • Author(s)
      小林大貴、淺川雅、福間剛士
    • Organizer
      第61回応用物理学会春季学術講演会
    • Place of Presentation
      青山学院大学
    • Year and Date
      2014-03-19
    • Related Report
      2013 Final Research Report
  • [Presentation] Nanoscale flexible chains at solid/liquid interfaces visualized by 3D-SFM2013

    • Author(s)
      H. Asakawa et al.
    • Organizer
      16th International Conference on non-contact Atomic Force Microscopy(nc-AFM2013)
    • Place of Presentation
      Maryland, USA
    • Year and Date
      2013-08-06
    • Related Report
      2013 Final Research Report
  • [Presentation] 周波数変調AFMによるリン脂質混合膜における相分離構造の3次元計測2013

    • Author(s)
      鈴木啓太、淺川雅、福間剛士
    • Organizer
      第60回応用物理学会春季学術講演会
    • Place of Presentation
      神奈川工科大学、神奈川県
    • Year and Date
      2013-03-28
    • Related Report
      2013 Final Research Report
  • [Presentation] 高分解能原子間力顕微鏡によるナノバイオ界面の3次元空間計測2013

    • Author(s)
      淺川雅
    • Organizer
      第4回 NMMSセミナー
    • Place of Presentation
      東京大学、東京都
    • Year and Date
      2013-03-01
    • Related Report
      2013 Final Research Report
  • [Presentation] 3D scanning force microscopy for subnanometer-scale investigations on biological interface2013

    • Author(s)
      H. Asakawa
    • Organizer
      Catalysis Research Center(CRC) International Symposium, New Challenges on the Bio-interfaces: Structures and Dynamics
    • Place of Presentation
      Hokkaido Univ., Hokkaido
    • Year and Date
      2013-02-06
    • Related Report
      2013 Final Research Report
  • [Presentation] 3次元走査型原子間力顕微鏡による脂質膜/水界面の分子分解能イメージング2013

    • Author(s)
      淺川雅
    • Organizer
      第22回インテリジェント材料/システムシンポジウム
    • Place of Presentation
      東京女子医科大学、東京都
    • Year and Date
      2013-01-08
    • Related Report
      2013 Final Research Report
  • [Presentation] 3D scanning force microscopy for subnanometer-scale investigations on biological interfaces2013

    • Author(s)
      H. Asakawa and T. Fukuma
    • Organizer
      CRC International Symposium, New Challenges on the Bio-interfaces: Structures and Dynamics
    • Place of Presentation
      Hokkaido University
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] 液中周波数変調 AFMのナノバイオ界面計測への応用2012

    • Author(s)
      淺川雅
    • Organizer
      第73回応用物理学会学術講演会
    • Place of Presentation
      松山大学、愛媛県
    • Year and Date
      2012-09-11
    • Related Report
      2013 Final Research Report
  • [Presentation] 液中高分解能原子間力顕微鏡の開発と生体分子計測への応用2012

    • Author(s)
      淺川雅
    • Organizer
      生物物質科学フォーラム
    • Place of Presentation
      北陸先端科学技術大学院大学、石川県
    • Year and Date
      2012-03-09
    • Related Report
      2013 Final Research Report
  • [Presentation] 液中周波数変調AFMのナノバイオ界面計測への応用2012

    • Author(s)
      淺川雅
    • Organizer
      第73回 応用物理学会秋季学術講演会
    • Place of Presentation
      松山大学
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] 液中高分解能原子間力顕微鏡の開発と生体分子計測への応用2012

    • Author(s)
      淺川雅
    • Organizer
      生物物質科学フォーラム(招待講演)
    • Place of Presentation
      北陸先端科学技術大学院大学, 石川県
    • Related Report
      2011 Research-status Report
  • [Presentation] Visualizing Molecular Arrangements at the Surface of Cylindrical Protein Structures by FM-AFM in Liquid2011

    • Author(s)
      H. Asakawa et al.
    • Organizer
      14th International Conference on Non-Contact Atomic Force Microscopy (NC-AFM2011)
    • Place of Presentation
      Lindau, Germany
    • Year and Date
      2011-09-19
    • Related Report
      2013 Final Research Report
  • [Presentation] Visualizing Molecular Arrangements at the Surface of Cylindrical Protein Structures by FM-AFM in Liquid2011

    • Author(s)
      H. Asakawa, Y. Katagiri, K. Ikegami, M. Setou, T. Fukuma
    • Organizer
      14th International Conference on Non-Contact Atomic Force Microscopy
    • Place of Presentation
      Inselhalle Lindau, Germany
    • Related Report
      2011 Research-status Report
  • [Presentation] Nanoscale flexible chains at solid/liquid interfaces visualized by 3D-SFM

    • Author(s)
      H. Asakawa, N. Inada and T. Fukuma
    • Organizer
      16th International Conference on non-contact Atomic Force Microscopy(nc-AFM2013)
    • Place of Presentation
      Maryland, USA
    • Related Report
      2013 Annual Research Report
  • [Presentation] 3次元走査型力顕微鏡による多層状脂質二重膜のナノスケール計測

    • Author(s)
      小林大貴、淺川雅、福間剛士
    • Organizer
      第61回応用物理学会春季学術講演会
    • Place of Presentation
      青山学院大学、神奈川県
    • Related Report
      2013 Annual Research Report
  • [Presentation] 高分解能原子間力顕微鏡によるナノバイオ界面の3次元空間計測

    • Author(s)
      淺川雅、稲田なつみ、片桐由智、鈴木啓太、福間剛士
    • Organizer
      第4回 NMMSセミナー
    • Place of Presentation
      東京大学
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] 3次元走査型原子間力顕微鏡による脂質膜/水界面の分子分解能イメージング

    • Author(s)
      淺川雅、鈴木啓太、福間剛士
    • Organizer
      第22回インテリジェント材料/システムシンポジウム
    • Place of Presentation
      東京女子医科大学
    • Related Report
      2012 Research-status Report
  • [Presentation] 周波数変調AFMによるリン脂質混合膜における相分離構造の3次元計測

    • Author(s)
      鈴木啓太、淺川雅、福間剛士
    • Organizer
      第60回応用物理学会春季学術講演会
    • Place of Presentation
      神奈川工科大学
    • Related Report
      2012 Research-status Report
  • [Remarks]

    • URL

      http://fukuma.w3.kanazawa-u.ac.jp/

    • Related Report
      2013 Final Research Report
  • [Patent(Industrial Property Rights)] 密閉型 AFMセル2012

    • Inventor(s)
      福間剛士、淺川雅、片桐由智
    • Industrial Property Rights Holder
      金沢大学
    • Industrial Property Rights Type
      特許
    • Filing Date
      2012-11-15
    • Related Report
      2013 Final Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] 密閉型AFMセル2011

    • Inventor(s)
      福間剛士、淺川雅、片桐由智
    • Industrial Property Rights Holder
      福間剛士、淺川雅、片桐由智
    • Industrial Property Number
      2011-249451
    • Filing Date
      2011
    • Related Report
      2011 Research-status Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

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