Bio-nanocapsules for multifunctional bio-imaging probes

• Project/Area Number: 23710143
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Nanomaterials/Nanobioscience
• Research Institution: Nagoya University
• Principal Investigator: IIJIMA Masumi 名古屋大学, 生命農学研究科, 研究員 (40390728)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: バイオナノカプセル / イムノアッセイ / マイクロアレイ / 生体分子 / バイオイメージングプローブ / 抗体の整列化 / 高感度化 / イムノセンサー

Research Abstract

Here, we developed the “IRODORI method” which can detect multiple antigens simultaneously, regardless of the animal species and subclasses of IgGs. Thus, bio-nanocapsules are expected to be a new platform for the multifunctional bio-imaging probes.

Research Products

• [Journal Article] Nanocapsule-based probe for evaluating the orientation of antibodies immobilized on a solid phase (2013)
  • Author(s): Iijima, M., et al. (他 4 名)
  • Journal Title: Analyst Volume: 138 Issue: 12 Pages: 3470-3477
  • DOI: 10.1039/c3an00481c
  • Peer Reviewed
• [Journal Article] A bio-nanocapsule containing envelope (E) protein domain III of Japanese encephalitis virus (JEV) protects mice against lethal JEV infection (2013)
  • Author(s): Miyata, T., et al.
  • Journal Title: Microbiology and Immunology, Accepted Pages: n/a-n/a
  • DOI: 10.1111/j.1348-0421.12055
  • Peer Reviewed
• [Journal Article] Bio-nanocapsules for signal enhancement of alkaline phosphatase-linked immunosorbent assays (2013)
  • Author(s): Iijima, M., et al. (他4名)
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 77 Pages: 843-846
  • NAID: 10031170729
  • Peer Reviewed
• [Journal Article] An automated system for high-throughput single cell-based breeding (2013)
  • Author(s): Nobuo Yoshimoto, Akiko Kida, Xu Jie, Masaya Kurokawa, Masumi Iijima, Tomoaki Niimi, Andrés D. Maturana, Itoshi Nikaido, Hiroki R. Ueda, Kenji Tatematsu, Katsuyuki Tanizawa, Akihiko Kondo, Ikuo Fujii, Shun'ichi Kuroda
  • Journal Title: Scientific Reports Volume: 3 Issue: 1 Pages: 1191-1191
  • DOI: 10.1038/srep01191
  • Peer Reviewed / Open Access
• [Journal Article] Cell surface-fluorescence immunosorbent assay for real-time detection of hybridomas with efficient antibody secretion at the single-cell level (2013)
  • Author(s): Kida, A.
  • Journal Title: Analytical Chemistry Volume: 85 Issue: 3 Pages: 1753-1759
  • DOI: 10.1021/ac303067k
  • Peer Reviewed
• [Journal Article] Nano-visualization of oriented-immobilized IgGs on immunosensors by high-speed atomic force microscopy (2012)
  • Author(s): Iijima, M., et al. (他 4 名)
  • Journal Title: Scientific Reports Volume: 2 Issue: 1 Pages: 790-790
  • DOI: 10.1038/srep00790
  • Peer Reviewed
• [Journal Article] Engineered bio-nanocapsules, hepatitis B virus surface antigen L protein particles, for in vivo active targeting to splenic dendritic cells (2012)
  • Author(s): Matsuo, H., et al.
  • Journal Title: International Journal of Nanomedicine Volume: 7 Pages: 3341-3350
  • DOI: 10.2147/ijn.s32813
  • Peer Reviewed
• [Journal Article] The C-terminal region of NELL1 mediates osteoblastic cell adhesion through integrin α3β1. (2012)
  • Author(s): Hasebe, A., et al.
  • Journal Title: FEBS Letters Volume: 586 Pages: 2500-2506
  • Peer Reviewed
• [Journal Article] Targeting of polyplex to human hepatic cells by bio-nanocapsules, hepatitis B Virus surface antigen L protein particles. (2012)
  • Author(s): Somiya, M., et al.
  • Journal Title: Bioorganic and Medicinal Chemistry Volume: 20 Pages: 3873-3879
  • Peer Reviewed
• [Journal Article] Hepatitis B virus envelope L protein-derived bio-nanocapsules: Mechanisms of cellular attachment and entry into human hepatic cells (2012)
  • Author(s): Yamada, M., et al.
  • Journal Title: Journal of Controlled Release Volume: 160 Pages: 322-329
  • Peer Reviewed
• [Journal Article] Efficient Production and Characterization of Recombinant Human NELL1 Protein in Human Embryonic Kidney 293-F Cells (2012)
  • Author(s): Hasebe A, Tashima H, Ide T, Iijima M, Yoshimoto N, Ting K, Kuroda S, Niimi T.
  • Journal Title: Molecular Biotechnology Volume: 51 Issue: 1 Pages: 58-66
  • DOI: 10.1007/s12033-011-9440-4
  • Peer Reviewed
• [Journal Article] Targeting of polyplex to human hepatic cells by bio-nanocapsules, hepatitis B Virus surface antigen L protein particles (2012)
  • Author(s): Somiya, M.
  • Journal Title: Bioorganic and Medicinal Chemistry Volume: 20 Pages: 3873-3879
  • Peer Reviewed
• [Journal Article] The C-terminal region of NELL1 mediates osteoblastic cell adhesion through integrin α3β1 (2012)
  • Author(s): Hasebe, A.
  • Journal Title: FEBS Letters Volume: 586 Pages: 2500-2506
  • Peer Reviewed
• [Journal Article] Fluorophore-labeled nanocapsules displaying IgG Fc-binding domains for the simultaneous detection of multiple antigens (2011)
  • Author(s): M.Iijima, T.Matsuzaki, N.Yoshimoto, T.Niimi, K.Tanizawa, S.Kuroda
  • Journal Title: Biomaterials Volume: 32 Issue: 34 Pages: 9011-9020
  • DOI: 10.1016/j.biomaterials.2011.08.012
  • Peer Reviewed
• [Journal Article] Efficient and rapid purification of drug- and gene-carrying bio-nanocapsules, hepatitis B virus surface antigen L particles (2011)
  • Author(s): Jung, J., et al.
  • Journal Title: Saccharomyces cerevisiae. Protein Expression and Purification Volume: 78 Issue: 2 Pages: 149-155
  • DOI: 10.1016/j.pep.2011.04.008
  • Peer Reviewed
• [Journal Article] Nanocapsules Incorporating IgG Fc-binding Domain Derived from Staphylococcus aureus Protein-A for Displaying IgGs on Immunosensor Chips (2011)
  • Author(s): M.Iijima, H.Kadoya, T.Matsuzaki, S.Hatahira, S.Hiramatsu, G.Jung, A.Martin, J.Quinn, J.Jung, S.-Y.Jeong, E.K.Choi, T.Arakawa, F.Hinako, M.Kusunoki, N.Yoshimoto, T.Niimi, K.Tanizawa, S.Kuroda
  • Journal Title: Biomaterials Volume: 32 Issue: 6 Pages: 1455-1464
  • DOI: 10.1016/j.biomaterials.2010.10.057
  • Peer Reviewed
• [Journal Article] Fluorophore-labeled nanocapsules displaying IgG Fc-binding domains forthe simultaneous detection of multiple antigens (2011)
  • Author(s): 飯嶋 益巳
  • Journal Title: Biomaterials Volume: 32
  • Peer Reviewed
• [Presentation] バイオセンサー表面におけるセンシング分子方向性のナノレベル制御 (2013)
  • Author(s): 飯嶋益巳
  • Organizer: 電気化学会第80回大会
  • Place of Presentation: 仙台
• [Presentation] バイオナノカプセルによるバイオセンサーのナノレベル表面制御及び高機能化 (2013)
  • Author(s): 飯嶋益巳
  • Organizer: 日本農芸化学会 2013 年度大会
  • Place of Presentation: 仙台
• [Presentation] Bio-nanocapsules for optimizing nanostructure of biosensor surface (2013)
  • Author(s): Iijima, M.
  • Organizer: 3rd International Conference on Bio-Sensing Technology
  • Place of Presentation: Sitges, Spain
• [Presentation] バイオナノカプセルによるバイオセンサーのナノレベル表面制御及び高機能化 (2013)
  • Author(s): 飯嶋 益巳
  • Organizer: 日本農芸化学会2013年度大会
  • Place of Presentation: 東北大学（宮城県）
• [Presentation] バイオセンサー表面におけるセンシング分子方向性のナノレベル制御 (2013)
  • Author(s): 飯嶋 益巳
  • Organizer: 電気化学会第80回大会
  • Place of Presentation: 東北大学（宮城県）
• [Presentation] Bio-nanocapsules for optimizing nanostructure of biosensor surface (2013)
  • Author(s): Iijima, M.
  • Organizer: 3rd International Conference on Bio-Sensing Technology
  • Place of Presentation: Melia Sitges（スペイン）
• [Presentation] バイオナノカプセルを用いた生体分子整列化技術によるバイオセンサーの高機能化 (2012)
  • Author(s): 飯嶋益巳
  • Organizer: 2012 年度日本生物工学会中部支部例会若手講演
  • Place of Presentation: 名古屋
  • Year and Date: 2012-08-02
• [Presentation] ワクチン抗原の能動的送達を可能にする樹状細胞標的化バイオナノカプセルの開発 (2012)
  • Author(s): 松尾英典
  • Organizer: 第28 会日本 DDS 学会学術集会
  • Place of Presentation: 札幌
• [Presentation] バイオナノカプセルを用いた抗体整列化技術によるバイオセンサーの高感度化 (2012)
  • Author(s): 飯嶋益巳
  • Organizer: 日本農芸化学会 2012 年度大会
  • Place of Presentation: 京都
• [Presentation] In vivo active delivery of antigens to splenic dendritic cells by engineered bio-nanocapsules, hepatitis B virus surface antigen L protein particles (2012)
  • Author(s): Matsuo, H
  • Organizer: Vaccine Technology IV
  • Place of Presentation: Albufeira, Portugal
• [Presentation] Bio-nanocapsules for a scaffold of oriented immobilization of antibodies in immunoassay systems (2012)
  • Author(s): Iijima, M
  • Organizer: Biosensors2012
  • Place of Presentation: Cancun, Mexico
• [Presentation] Bio-nanocapsules as a scaffold of oriented immobilization of antibodies in highly sensitive immunoassay systems (2012)
  • Author(s): Iijima, M
  • Organizer: International Symposium on Innovative Nanobiodevices ISIN2012
  • Place of Presentation: Nagoya, Japan
• [Presentation] A new platform for bio-imaging probes based on oriented immobilization of biological sensing molecules (2012)
  • Author(s): Iijima, M
  • Organizer: The 15th SANKEN International Symposium 2012, The 10th SANKEN Nanotechnology Symposium Progress and Perspectives of Imaging Science and Technology-From Nano Structure To Human Behavior
  • Place of Presentation: Osaka, Japan
• [Presentation] Bio-nanocapsules for a scaffold of oriented immobilization of antibodies in immunoassay systems (2012)
  • Author(s): Iijima, M.
  • Organizer: Biosensors2012
  • Place of Presentation: Peninsula Convention Centre（メキシコ）
• [Presentation] In vivo active delivery of antigens to splenic dendritic cells by engineered bio-nanocapsules, hepatitis B virus surface antigen L protein particles (2012)
  • Author(s): Matsuo, H.
  • Organizer: Vaccine Technology IV
  • Place of Presentation: Grande Real Santa Eulalia Hotel（ポルトガル）
• [Presentation] ワクチン抗原の能動的送達を可能にする樹状細胞標的化バイオナノカプセルの開発 (2012)
  • Author(s): 松尾 英典
  • Organizer: 第28会日本DDS学会学術集会
  • Place of Presentation: 札幌コンベンションセンター（北海道）
• [Presentation] バイオナノカプセルを用いた生体分子整列化技術によるバイオセンサーの高機能化 (2012)
  • Author(s): 飯嶋 益巳
  • Organizer: 2012年度日本生物工学会中部支部例会若手講演
  • Place of Presentation: 名古屋大学（愛知県）
• [Presentation] A New Platform for Bio-imaging Probes Based on Oriented Immobilization of Biological Sensing Molecules (2012)
  • Author(s): 飯嶋 益巳
  • Organizer: The 15th SANKEN International Symposium 2012, The 10th SANKEN Nanotechnology Symposium Progress and Perspectives of Imaging Science and Technology-From Nano Structure To Human Behavior(招待講演)
  • Place of Presentation: 大阪大学 (大阪府)
• [Presentation] Bio-nanocapsules as a scaffold of oriented immobilization of antibodies in highly sensitive immunoassay systems (2012)
  • Author(s): 飯嶋 益巳
  • Organizer: International symposium on innovative nanobiodevices ISIN2012
  • Place of Presentation: 名古屋大学 (愛知県)
• [Presentation] バイオナノカプセルを用いた抗体整列化技術によるバイオセンサーの高感度化 (2012)
  • Author(s): 飯嶋 益巳
  • Organizer: 日本農芸化学会2012年度大会
  • Place of Presentation: 京都女子大学 (京都府)
• [Presentation] Bio-nanocapsules for a scaffold of oriented immobilization of antibodies in immunoassay systems (2012)
  • Author(s): 飯嶋 益巳
  • Organizer: Biosensors2012
  • Place of Presentation: Peninsula Convention Centre (メキシコ)
• [Presentation] 新しい抗体整列化技術(バイオナノカプセル)による高機能バイオセンサーの開発 (2011)
  • Author(s): 飯嶋益巳
  • Organizer: 第63回日本生物工学会大会
  • Place of Presentation: 東京
• [Presentation] Bio-nanocapsules for oriented Immobilization of IgGs on immunosensor chips (2011)
  • Author(s): Iijima, M
  • Organizer: 5th European Conference of the International Federation for Medical and Biological Engineering
  • Place of Presentation: Budapest, Hungary
• [Presentation] Bio-nanocapsules for oriented immobilization of IgGs on immunosensor chips (2011)
  • Author(s): 飯嶋 益巳
  • Organizer: 5th European Conference of the International Federation for Medical and Biological Engineering
  • Place of Presentation: Budapest Congress & World Trade Center (ハンガリー)
• [Presentation] 新しい抗体整列化技術（バイオナノカプセル）による高機能バイオセンサーの開発 (2011)
  • Author(s): 飯嶋 益巳
  • Organizer: 第63回日本生物工学会大会
  • Place of Presentation: 東京農工大学 (東京都)
• [Book] バイオサイエンスとバイオインダストリー（B&I） (2013)
  • Author(s): 飯嶋 益巳
  • Total Pages: 4
  • Publisher: 一般財団法人 バイオインダストリー協会
• [Book] Bio-nanocapsules for oriented immobilization of IgGs on immunosensor Chips. A. Jobbagy (Ed.) (2012)
  • Author(s): Iijima, M., et al. (他 1 名)
  • Publisher: Springer-Verlag
• [Book] 5th European Conference of the International Federation for Medical and Biological Engineering 14-18 September 2011, Budapest, Hungary (2012)
  • Author(s): 飯嶋 益巳
  • Total Pages: 1466
  • Publisher: Springer-Verlag
• [Book] Nanocapsules incorporating IgG Fc-binding domain derived from Staphylococcus aureus protein-A for displaying IgGs on immunosensor chips (2012)
  • Author(s): 飯嶋 益巳
  • Total Pages: 63
  • Publisher: 大阪大学
• [Book] バイオナノカプセルを用いるセンシング分子整列化技術
  • Author(s): 飯嶋ら、(他 1 名)
  • Publisher: 一般財団法人バイオインダストリー協会(印刷中)
• [Remarks]
  • URL: http://www.natureasia.com/ja-jp/srep/abstracts/40899
• [Remarks]
  • URL: http://www.natureasia.com/ja-jp/srep/abstracts/42558
• [Remarks]
  • URL: http://www.sanken.osaka-u.ac.jp/jp/operation/research_activities.html
• [Remarks]
  • URL: http://www.beacle.com/japanese/top.html
• [Remarks]
  • URL: http://www.agr.nagoya-u.ac.jp/~tagen/tagen/home.html
• [Remarks] 名古屋大学産業生命工学研究室
  • URL: http://www.agr.nagoya-u.ac.jp/~tagen/tagen/home.html
• [Remarks]
  • URL: http://www.sanken.osaka-u.ac.jp/jp/operation/research_activities.html#oct2011
• [Patent(Industrial Property Rights)] バイオナノカプセル及びリポソームを含む複合体 (2012)
  • Inventor(s): 黒田俊一、松尾英典、良元伸男、飯嶋益巳、新川武
  • Industrial Property Rights Holder: 黒田俊一、松尾英典、良元伸男、飯嶋益巳、新川武
  • Industrial Property Number: 2012-080791
  • Filing Date: 2012-03-30
• [Patent(Industrial Property Rights)] 多抗原同時検出用ナノ粒子 (2010)
  • Inventor(s): 黒田俊一、松崎高志、飯嶋益巳
  • Industrial Property Rights Holder: 黒田俊一、松崎高志、飯嶋益巳
  • Patent Publication Number: 2010-210444
  • Acquisition Date: 2010-09-24