Development of optically-driven tool for nano-bio processing and its applications in molecular dissection of genomic DNA
| Project/Area Number |
23710145
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Nanomaterials/Nanobioscience
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| Research Institution | Kagawa University |
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Principal Investigator |
TERAO Kyohei 香川大学, 工学部, 准教授 (80467448)
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| Co-Investigator(Renkei-kenkyūsha) |
OANA Hidehiro 東京大学, 大学院・工学系研究科, 准教授 (20314172)
OOHIRA Fumikazu 香川大学, 理事 (80325315)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | 分子マニピュレーション / 分子加工 / DNA / 分子操作 / 光ピンセット / ゲノム / バイオナノテクノロジー / 一分子操作 / ＭＥＭＳ / マイクロＴＡＳ |
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| Research Abstract |
In this research project, we developed a novel nano/micro-fabricated tool for processing single biomolecule for the application in the research field of genome analysis. The technique for fabricating nano/micro structures was developed, which enables us to control the visibility of the structures under a fluorescence microscope. Their characteristics including the yield of fabrication and the trapping force were evaluated. We also verified the immobilization of proteins on their surface by surface plasmon resonance sensing. The results suggest the successful protein immobilization and the functionalization for a protein-modified nano/micro tool.
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Report
(3 results)
Research Products
(17 results)
