Formation and measurement of arrayed lipid bilayer membranes via accumulation of aqueous plugs in a microfluidic device
| Project/Area Number |
23710146
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Microdevices/Nanodevices
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
NISISAKO Takasi 東京工業大学, 精密工学研究所, 助教 (10431983)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | ナノバイオ / マイクロ・ナノデバイス / 流体工学 / 脂質二分子膜 / マイクロ流路 / 液滴 / 膜タンパク質 / 脂質 |
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| Research Abstract |
We studied formation of planar lipid bilayer membranes and their electrophysiological analysis by using microfluidic channels fabricated on a chip. Nanoliter-sized aqueous droplets were produced at T- or cross-junctions into an organic stream containing lipid molecules. At the downstream, the organic phase was selectively aspirated and removed, and those aqueous droplets were brought into contact, forming arrayed droplet interface bilayers (DIBs). Furthermore, electrical measurement on the formed DIBs and incorporated channels was also demonstrated.
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Report
(4 results)
Research Products
(10 results)
