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Formation and measurement of arrayed lipid bilayer membranes via accumulation of aqueous plugs in a microfluidic device

Research Project

Project/Area Number 23710146
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Microdevices/Nanodevices
Research InstitutionTokyo Institute of Technology

Principal Investigator

NISISAKO Takasi  東京工業大学, 精密工学研究所, 助教 (10431983)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywordsナノバイオ / マイクロ・ナノデバイス / 流体工学 / 脂質二分子膜 / マイクロ流路 / 液滴 / 膜タンパク質 / 脂質
Research Abstract

We studied formation of planar lipid bilayer membranes and their electrophysiological analysis by using microfluidic channels fabricated on a chip. Nanoliter-sized aqueous droplets were produced at T- or cross-junctions into an organic stream containing lipid molecules. At the downstream, the organic phase was selectively aspirated and removed, and those aqueous droplets were brought into contact, forming arrayed droplet interface bilayers (DIBs). Furthermore, electrical measurement on the formed DIBs and incorporated channels was also demonstrated.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (10 results)

All 2013 2012 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (7 results) (of which Invited: 1 results) Remarks (1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Microfluidic passive permeability assay using nanoliter droplet interface bilayers2013

    • Author(s)
      Takasi Nisisako, Shiva A. Portonovo, and Jacob J. Schmidt
    • Journal Title

      Analyst

      Volume: Vol.138 Issue: 22 Pages: 6793-6800

    • DOI

      10.1039/c3an01314f

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Presentation] Microfluidic droplet-based drug permeability assay2013

    • Author(s)
      Takasi Nisisako
    • Organizer
      2013 International Conference on Small Science (ICSS2013)
    • Place of Presentation
      Las Vegas, NV, USA
    • Year and Date
      2013-12-16
    • Related Report
      2013 Final Research Report
  • [Presentation] Microfluidic passive permeability assay using nanoliter droplet interface lipid bilayers2013

    • Author(s)
      Takasi Nisisako, Shiva A. Portonovo, and Jacob J. Schmidt
    • Organizer
      Biophysical society 57th Annual Meeting
    • Place of Presentation
      Philadlphia, PA, USA
    • Related Report
      2013 Final Research Report
  • [Presentation] Microfluidic passive permeability assay using nanoliter droplet interface lipid bilayers2013

    • Author(s)
      Takasi Nisisako, Shiva A. Portonovo, and Jacob J. Schmidt
    • Organizer
      Biophysical society 57th Annual Meeting
    • Place of Presentation
      Philadelphia, PA, U.S.A.
    • Related Report
      2012 Research-status Report
  • [Presentation] Droplet microfluidics for manufacturing and analytical applications2012

    • Author(s)
      Takasi Nisisako
    • Organizer
      2012 International Conference on Small Science (ICSS)
    • Place of Presentation
      Orlando, FL, USA
    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Presentation] Microfluidic passive permeability assay using arrayed droplet interface membranes2012

    • Author(s)
      Takasi Nisisako, Shiva A. Portonovo, and Jacob J. Schmidt
    • Organizer
      The 16th international Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2012)
    • Place of Presentation
      Okinawa, Japan
    • Related Report
      2013 Final Research Report
  • [Presentation] Microfluidic passive permeability assay using arrayed droplet interface membranes2012

    • Author(s)
      Takasi Nisisako, Shiva A. Portonovo, and Jacob J. Schmidt
    • Organizer
      The 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2012)
    • Place of Presentation
      Okinawa, Japan
    • Related Report
      2012 Research-status Report
  • [Presentation] Microfulidic droplet-based drug permeability assay

    • Author(s)
      Takasi Nisisako
    • Organizer
      2013 International Conference on Small Science (ICSS2013)
    • Place of Presentation
      Las Vegas, NV, USA
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Remarks]

    • URL

      http://www.pme.pi.titech.ac.jp/staff/nisisako/index.html

    • Related Report
      2013 Final Research Report
  • [Patent(Industrial Property Rights)] 二分子膜の製造方法および二分子平面膜2012

    • Inventor(s)
      西迫貴志,馬場崇弘
    • Industrial Property Rights Holder
      国立大学法人東京工業大学
    • Industrial Property Rights Type
      特許
    • Acquisition Date
      2012-08-10
    • Related Report
      2013 Final Research Report

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Published: 2011-08-05   Modified: 2019-07-29  

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