Electrophysiological recording using Spatially Arranged Microelectrode Embedded into 3D Culture

• Project/Area Number: 23710151
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Microdevices/Nanodevices
• Research Institution: Ritsumeikan University
• Principal Investigator: TONOMURA Wataro 立命館大学, 理工学部, 助教 (50581493)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 空間配列プローブ電極 / 3 次元培養 / マイクロバイオシステム / 3次元培養組織 / 包埋計測

Research Abstract

3D cultures are more likely to mimic physiological tissue environments. This research focuses on spatialextracellular recordings inside 3D cultures using spatially arranged microelectrode embedded into 3Dcultures. Wire?bonding?based probe technology makes it possible to provide the 3D microelectrodes.We demonstrated electrophysiological activities inside 3D neuronal cultures could be successfullyrecorded using the embedded microelectrodes. We also indicated that cell manipulation, optical sensingand electrochemical sensing were integrated with the spatially arranged microelectrode.

Research Products

• [Presentation] High-Sensitivity Electrochemical Sensor using Pyrolyzed Polymer-Gold 3D Probe Arrays for Spatial Chemical Sensing (2013)
  • Author(s): W. Tonomura, Y. Mori and S. Konishi
  • Organizer: The 26th IEEE International Conference on Micro Electro Mechanical Systems (MEMS2013)
  • Place of Presentation: Taipei, Taiwan
• [Presentation] Hetero Multilayer Structures by Rapid Prototyping for Simultaneous Encapsulation and Interconnection of Microchips (2013)
  • Author(s): S. Katano, Y. Teramachi, W. Tonomura and S. Konishi
  • Organizer: The 26th IEEE International Conference on Micro Electro Mechanical Systems (MEMS2013)
  • Place of Presentation: Taipei, Taiwan
• [Presentation] Magneticmicroprobes spatially arranged inculture gel for 3D patterning ofmagnetically labeled cells (2012)
  • Author(s): J. Sato, W. Tonomurand S.Konishi
  • Organizer: The 6th Asia-Pacific Conference onTransducers and Micro/NanoTechnologies (APCOT2012)
  • Place of Presentation: 南京(中国)
  • Year and Date: 2012-07-09
• [Presentation] High-sensitivitelectrochemical sensor usingpyrolyzed polymer-gold 3D probe arfor spatial chemical sensing (2012)
  • Author(s): W. Tonomura, Y. Mori and S. Konishi
  • Organizer: The 26th IEEE InternationalConference on Micro Electro MechanSystems (MEMS2013)
  • Place of Presentation: 台北(台湾)
• [Presentation] Magnetic Microprobes Spatially Arranged in Culture Gel for 3D Patterning of Magnetically Labeled Cells (2012)
  • Author(s): J. Sato, W. Tonomura and S. Konishi
  • Organizer: The 6th Asia-Pacific Conference on Transducers and Micro/Nano Technologies (APCOT2012)
  • Place of Presentation: Nanjing, China
• [Presentation] Multi-site electroretinogramrecordings inside isolated mouseusing flexible spatiallyarranged microelectrode probes (2011)
  • Author(s): W. Tonomura, S. Taga, C.Koike and S. Konishi
  • Organizer: The 16th InternationalConference on Solid-State Sensors,Actuators and Microsystems(Transducers2011
  • Place of Presentation: 北京(中国
  • Year and Date: 2011-06-07
• [Presentation] 3次元培養組織解析のための空間配列マイクロ電極を用いた包埋計測 (2011)
  • Author(s): 殿村渉,五十嵐悠太,清一憲,小西聡
  • Organizer: 日本機械学会ロボティクス・メカトロニクス講演会
  • Place of Presentation: 岡山コンベンションセンター(岡山県)
  • Year and Date: 2011-05-27
• [Presentation] Multi-site electroretinogram recordings inside isolated mouse retina using flexible spatially arranged microelectrode probes (2011)
  • Author(s): W. Tonomura, S. Taga, C. Koike and S. Konishi
  • Organizer: The 16th International Conference on Solid-State Sensors, Actuators and Microsystems (Transducers2011)
  • Place of Presentation: Beijing, China
• [Presentation] Parallel focusing and switching of leakage flux from magnetic loop for on-chip patterning of magnetically labeled cells (2011)
  • Author(s): J. Kunishida, W. Tonomura, J. Sato and S. Konishi
  • Organizer: The 16th International Conference on Solid-State Sensors, Actuators and Microsystems (Transducers2011)
  • Place of Presentation: Beijing, China
• [Presentation] 空間配列マイクロ電極を用いた局所網膜電位図の多チャンネル計測 (2011)
  • Author(s): 殿村 渉、多賀 駿、小池 千恵子、小西 聡
  • Organizer: 第28回「センサ・マイクロマシンと応用システム」シンポジウム
  • Place of Presentation: タワーホール船堀（東京都）
• [Presentation] 3次元培養組織解析のための空間配列マイクロ電極を用いた包埋計測 (2011)
  • Author(s): 殿村 渉、五十嵐 悠太、清水 一憲、小西 聡
  • Organizer: 日本機械学会 ロボティクス・メカトロニクス講演会（ROBOMEC2011）
  • Place of Presentation: 岡山コンベンションセンター（岡山県）
• [Book] 食のバイオ計測の最前線機能解析と安全・安心の計測を目指して(4バイオセンサーデバイスにおけるサンプル前処理技術 (2011)
  • Author(s): 小西聡,小林大造,殿村渉,清水一憲
  • Publisher: シーエムシー出版
• [Book] 食のバイオ計測の最前線-機能解析と安全・安心の計測を目指して-（4 バイオセンサーデバイスにおけるサンプル前処理技術） (2011)
  • Author(s): 小西 聡,、小林 大造、殿村 渉、清水 一憲
  • Publisher: シーエムシー出版
• [Remarks] 立命館大学理工学部機械工学科（小西・殿村研究室）
  • URL: http://www.ritsumei.ac.jp/se/~konishi/mems/home.htm