DNA methylation analysis by surface plasmon resonance on a microhole array

• Project/Area Number: 23710152
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Microdevices/Nanodevices
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: KURITA Ryoji 独立行政法人産業技術総合研究所, バイオメディカル研究部門, 主任研究員 (50415676)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: DNA / メチルシトシン / エピジェネティクス

Research Abstract

We report the sequence-selective discrimination of the cytosine methylation status in DNA with anti methylcytosine antibody for the first time. This was realized by employing an affinity measurement involving the target methylcytosine in a bulge region an

Research Products

• [Journal Article] DNA Methylation Analysis Triggered by Bulge Specific Immuno-Recognition (2012)
  • Author(s): Ryoji Kurita and Osamu Niwa
  • Journal Title: Analytical Chemistry Volume: 84 Issue: 17 Pages: 7533-7538
  • DOI: 10.1021/ac301702y
• [Journal Article] Electrochemical Surface Plasmon Resonance Measurement Based on Gold Nanohole Array Fabricated by Nanoimprinting Technique, Analytical Chemistry (2012)
  • Author(s): Ryoji Kurita, Kumi Arai, Kohei Nakamoto, Dai Kato, Osamu Niwa
  • Journal Title: Analytical Chemistry Volume: (In press) Issue: 4 Pages: 1799-1803
  • DOI: 10.1021/ac202692f
  • Peer Reviewed
• [Journal Article] Development of a mass-producible on-chip plasmonic nanohole array biosensor (2011)
  • Author(s): Kohei Nakamoto, Ryoji Kurita, Osamu Niwa, Toshiyuki Fujii, Munehiro Nishida
  • Journal Title: Nanoscale Volume: 3 Issue: 12 Pages: 5067-5075
  • DOI: 10.1039/c1nr10883b
  • Peer Reviewed
• [Presentation] DNA バルジを利用したシーケンス選択的なメチルシトシン免疫測定法 (2013)
  • Author(s): 栗田僚二、丹羽修
  • Organizer: 電気化学会
  • Place of Presentation: 東北大学(宮城県)
  • Year and Date: 2013-03-31
• [Presentation] Single methylation analysis in DNA by electrochemiluminescence and surface plasmon resonance (2013)
  • Author(s): Ryoji Kurita
  • Organizer: Pittcon2013
  • Place of Presentation: Pennsylvania Convention Center Philadelphia(米国)
  • Year and Date: 2013-03-20
• [Presentation] DNAバルジを利用したシーケンス選択的なメチルシトシン免疫測定法 (2013)
  • Author(s): 栗田僚二
  • Organizer: 電気化学会
  • Place of Presentation: 東北大学
• [Presentation] Single methylation analysis in DNA by electrochemiluminescence and surface plasmon resonance (2013)
  • Author(s): 栗田僚二
  • Organizer: Pittcon2013
  • Place of Presentation: Philadelphia
  • Invited
• [Presentation] DNA バルジ特異的な抗体認識を利用したシトシンのメチル化診断 (2012)
  • Author(s): 栗田僚二、丹羽修
  • Organizer: 分析化学会
  • Place of Presentation: 金沢大学(石川県)
  • Year and Date: 2012-09-19
• [Presentation] DNAバルジ特異的な抗体認識を利用したシトシンのメチル化診断 (2012)
  • Author(s): 栗田僚二
  • Organizer: 分析化学会
  • Place of Presentation: 金沢大学
• [Presentation] Development of Electrogenerated Chemiluminescence based Enzyme-Linked Immunosorbent Assay (2011)
  • Author(s): 栗田僚二 ほか
  • Organizer: ICAS2011
  • Place of Presentation: 京都、京都国際会議場
• [Presentation] ENZYME LINKED IMMUNO-SORBENT ASSAY WITH SURFACE ACCUMULABLE MOLECULE FORMED BY LABELING ENZYME (2011)
  • Author(s): 栗田僚二
  • Organizer: 9th Asian Conference on Chemical Sensors(招待講演)
  • Place of Presentation: 台湾,Chientan Youth Activity Center
• [Presentation] Development of Electrochemiluminescence and Surface Plasmon Resonance based Immunosensors with Surface Accumulable Molecules (2011)
  • Author(s): 栗田僚二
  • Organizer: 2011 MRS fall meeting(招待講演)
  • Place of Presentation: 米国, Marriotto copley place
• [Book] メチル化 DNA の迅速検出を目指して (2013)
  • Author(s): 栗田僚二
  • Publisher: 化学とマイクロ・ナノシステム研究会誌
• [Book] 化学とマイクロ・ナノシステム研究会誌 (2013)
  • Author(s): 栗田僚二
  • Total Pages: 8
  • Publisher: 化学とマイクロ・ナノシステム研究会
• [Remarks]
  • URL: http://unit.aist.go.jp/biomed-ri/biomed-nbd/menber_kurita/kurita.html
• [Remarks]
  • URL: http://unit.aist.go.jp/biomed-ri/biomed-nbd/menber_kurita/kurita.html
• [Patent(Industrial Property Rights)] (英語)METHYLCYTOSINE DETECTION METHOD (和訳)メチルシトシン検出法 (2018)
  • Inventor(s): 栗田 僚二, 吉岡 恭子, 丹羽 修
  • Industrial Property Rights Holder: 産業技術総合研究所
  • Industrial Property Rights Type: 特許
  • Acquisition Date: 2018
  • Overseas
• [Patent(Industrial Property Rights)] METHYLCYTOSINE DETECTION METHOD (2015)
  • Inventor(s): 栗田 僚二
  • Industrial Property Rights Holder: 産業技術総合研究所
  • Industrial Property Rights Type: 特許
  • Filing Date: 2015-12-09
  • Overseas
• [Patent(Industrial Property Rights)] メチルシトシン検出法 (2014)
  • Inventor(s): 栗田 僚二, 柳澤 博幸, 吉岡 恭子, 丹羽 修
  • Industrial Property Rights Holder: 独立行政法人産業技術総合研究所
  • Industrial Property Rights Type: 特許
  • Filing Date: 2014-03-13
  • Overseas
• [Patent(Industrial Property Rights)] メチルシトシン検出法 (2013)
  • Inventor(s): 栗田僚二、丹羽修
  • Industrial Property Rights Holder: 栗田僚二、丹羽修
  • Industrial Property Number: 2013-051501
  • Filing Date: 2013-03-14
• [Patent(Industrial Property Rights)] メチルシトシン検出法 (2013)
  • Inventor(s): 栗田 僚二, 柳澤 博幸
  • Industrial Property Rights Holder: 産総研
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2013-051501
  • Filing Date: 2013-03-14
  • Acquisition Date: 2017-02-24
• [Patent(Industrial Property Rights)] メチル化核酸検出法 (2011)
  • Inventor(s): 栗田僚二、加藤大、丹羽修
  • Industrial Property Rights Holder: 栗田僚二、加藤大、丹羽修
  • Industrial Property Number: 2011-098844
  • Filing Date: 2011-04-27