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Formation of Size-controlled Giant Unilamellar Liposomes

Research Project

Project/Area Number 23710154
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Microdevices/Nanodevices
Research Institution公益財団法人神奈川科学技術アカデミー (2012)
Kanagawa Academy of Science and Technology (2011)

Principal Investigator

OSAKI Toshihisa  公益財団法人神奈川科学技術アカデミー, バイオマイクロシステムプロジェクト, 研究員 (50533650)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Keywords脂質 / 生体材料(リポソーム) / 人工細胞膜 / マイクロアレイ / マイクロ・ナノデバイス / 生体材料(リポソーム)
Research Abstract

In this study, we developed a method that allows formation of a uniform-size giant unilamellar liposome array. Microfabrication technologies and an electrospray technique enabled precise patterning of lipids on a substrate; with a simple hydration process of the lipid pattern, we succeeded in highly reproducible giant liposome array formation with a narrow range of size distribution. The formed liposomes were well suitable for model cell membrane studies. We further developed manipulation devices for the liposomes based on dielectrophoresis phenomena.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (24 results)

All 2013 2012 2011 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (19 results) (of which Invited: 1 results) Remarks (4 results)

  • [Journal Article] Round-tip dielectrophoresis-based tweezers for single micro-object manipulation2013

    • Author(s)
      Taiga Kodama, Toshihisa Osaki, Ryuji Kawano, Koki Kamiya, Norihisa Miki, and Shoji Takeuchi
    • Journal Title

      Biosensors and Bioelectronics

      Volume: 47 Pages: 206-212

    • DOI

      10.1016/j.bios.2013.03.022

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Presentation] Pendant Liposome System to Access the Internal Solution2013

    • Author(s)
      Toshihisa Osaki
    • Organizer
      The 26th IEEE International Conference on Micro Electro Mechanical Systems
    • Place of Presentation
      Taipei, Taiwan
    • Year and Date
      2013-01-24
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Presentation] Contactless Catch-and- Release System for Giant Liposomes Based on Negative Dielectrophoresis2013

    • Author(s)
      Taiga Kodama
    • Organizer
      The 26th IEEE International Conference on Micro Electro Mechanical Systems
    • Place of Presentation
      Taipei, Taiwan
    • Year and Date
      2013-01-23
    • Related Report
      2012 Final Research Report
  • [Presentation] Contactless Catch-and-Release System for Giant Liposomes Based on Negative Dielectrophoresis2013

    • Author(s)
      Taiga Kodama
    • Organizer
      The 26th IEEE International Conference on Micro Electro Mechanical Systems
    • Place of Presentation
      Taipei, Taiwan
    • Related Report
      2012 Annual Research Report
  • [Presentation] Artificial Cellular Membrane Microchip for Membrane Protein Analysis2012

    • Author(s)
      Toshihisa Osaki
    • Organizer
      The 10th International Conference on Nano-Molecular Electronics
    • Place of Presentation
      兵庫(招待講演)
    • Year and Date
      2012-12-12
    • Related Report
      2012 Final Research Report
  • [Presentation] Smooth-tip Dielectrophoresis Based Tweezers for Single Liposome Handling2012

    • Author(s)
      Taiga Kodama
    • Organizer
      The 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences
    • Place of Presentation
      沖縄
    • Year and Date
      2012-10-30
    • Related Report
      2012 Final Research Report
  • [Presentation] Uniform Size Liposome on a Chip: Observation of Transport Kinetics through Nanopore Membrane Protein2012

    • Author(s)
      Toshihisa Osaki
    • Organizer
      The 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences
    • Place of Presentation
      沖縄
    • Year and Date
      2012-10-29
    • Related Report
      2012 Final Research Report
  • [Presentation] 静置水和法による均一径リポソームアレイ2012

    • Author(s)
      大崎寿久
    • Organizer
      第50回日本生物物理学会年会
    • Place of Presentation
      名古屋
    • Year and Date
      2012-09-24
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Presentation] 静置水和法により形成した均一サイズリポソームアレイ応用技術2012

    • Author(s)
      大崎寿久
    • Organizer
      日本膜学会第34年会
    • Place of Presentation
      東京
    • Year and Date
      2012-05-08
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Presentation] Towards Artificial Cell Array System: Encapsulation and Hydration Technologies Integrated in Liposome Array2012

    • Author(s)
      Toshihisa Osaki
    • Organizer
      The 25th International Conference on Micro Electro Mechanical Systems
    • Place of Presentation
      Paris, France
    • Year and Date
      2012-01-31
    • Related Report
      2012 Final Research Report
  • [Presentation] Dielectrophoresis-Based Tweezers for Cell-Sized Liposome Manipulation2012

    • Author(s)
      Taiga Kodama
    • Organizer
      The 25th International Conference on Micro Electro Mechanical Systems
    • Place of Presentation
      Paris, France
    • Year and Date
      2012-01-31
    • Related Report
      2012 Final Research Report
  • [Presentation] Uniform Size Liposome on a Chip: Observation of Transport Kinetics through Nanopore Membrane Protein2012

    • Author(s)
      Toshihisa Osaki
    • Organizer
      The 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences
    • Place of Presentation
      沖縄
    • Related Report
      2012 Annual Research Report
  • [Presentation] Smooth-tip Dielectrophoresis Based Tweezers for Single Liposome Handling2012

    • Author(s)
      Taiga Kodama
    • Organizer
      The 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences
    • Place of Presentation
      沖縄
    • Related Report
      2012 Annual Research Report
  • [Presentation] Artificial Cellular Membrane Microchip for Membrane Protein Analysis2012

    • Author(s)
      Toshihisa Osaki
    • Organizer
      The 10th International Conference on Nano-Molecular Electronics
    • Place of Presentation
      兵庫
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Towards Artificial Cell Array System: Encapsulation and Hydration Technologies Integrated in Liposome Array2012

    • Author(s)
      Toshihisa Osaki
    • Organizer
      The 25th International Conference on Micro Electro Mechanical Systems (IEEE MEMS 2012)
    • Place of Presentation
      Marriott Paris Rive Gauche Hotel, Paris, France
    • Related Report
      2011 Research-status Report
  • [Presentation] Dielectrophoresis-Based Tweezers for Cell-Sized Liposome Manipulation2012

    • Author(s)
      Taiga Kodama
    • Organizer
      The 25th International Conference on Micro Electro Mechanical Systems (IEEE MEMS 2012)
    • Place of Presentation
      Marriott Paris Rive Gauche Hotel, Paris, France
    • Related Report
      2011 Research-status Report
  • [Presentation] Selective Lipid-Patterning for Heterologous Giant Liposome Array2011

    • Author(s)
      Toshihisa Osaki
    • Organizer
      The 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences
    • Place of Presentation
      Seattle, USA
    • Year and Date
      2011-10-04
    • Related Report
      2012 Final Research Report
  • [Presentation] 水和法を用いた均一サイズリポソームの形成2011

    • Author(s)
      大崎寿久
    • Organizer
      日本膜学会第33年会
    • Place of Presentation
      東京
    • Year and Date
      2011-05-13
    • Related Report
      2012 Final Research Report
  • [Presentation] 水和法を用いた均一サイズリポソームの形成2011

    • Author(s)
      大崎寿久
    • Organizer
      日本膜学会第33年会
    • Place of Presentation
      産総研臨海副都心センター(東京都)
    • Related Report
      2011 Research-status Report
  • [Presentation] Selective Lipid-Patterning for Heterologous Giant Liposome Array2011

    • Author(s)
      Toshihisa Osaki
    • Organizer
      The 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2011)
    • Place of Presentation
      Washington Convention Center, Seattle, Washington, USA
    • Related Report
      2011 Research-status Report
  • [Remarks]

    • URL

      http://www.newkast.or.jp/innovation/labo/takeuchi_project.html

    • Related Report
      2012 Final Research Report
  • [Remarks] 公益財団法人神奈川科学技術アカデミー 人工細胞膜システムグループ

    • URL

      http://www.newkast.or.jp/innovation/labo/takeuchi_project.html

    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://www.newkast.or.jp/innovation/labo/takeuchi_project.html

    • Related Report
      2011 Research-status Report
  • [Remarks] (論文掲載後に随時、追加予定)

    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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