The chromosomal territory influencing the regulation of gene expression in neuronal cells.

• Project/Area Number: 23710215
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Genome biology
• Research Institution: Kanazawa University
• Principal Investigator: HORIKE Shinichi 金沢大学, 学際科学実験センター, 准教授 (40448311)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 自閉症 / インプリンティング / PWS-IC / 染色体ペアリング / 核内配置 / 染色体テリトリー / エピジェネティクス / ゲノムインプリンティング / クロマチンループ / コピー数多型 / トランスクロモソミックマウス / ヒト15番染色体 / DNAメチル化 / TCマウス

Research Abstract

I examined the chromosome territory of a human chromosome 15 in neuronal cells by using the chromosome engineering techniques. Finally, I found that active gene region of 15q11-q13 was looping out from the chromosome 15 territory. From my results, 15q11-q13 locus was highly coordinately regulated by PWS-IC.

Research Products

• [Journal Article] Epigenetic defects related toassisted reproductive technologies: Large offspring syndrome (LOS) (2012)
  • Author(s): Nagai M., Meguro-Horike M., HorikeS.
  • Journal Title: DNA Methylation-Genomic Technologies and Impact Pages: 167-182
  • DOI: 10.5772/34102
  • ISBN: 9789535103202
• [Journal Article] Epigenetic defects related to assisted reproductive technologies: Large offspring syndrome (LOS) (2012)
  • Author(s): Nagai M.
  • Journal Title: DNA Methylation-From Genomics to Technology Volume: なし Pages: 167-182
• [Journal Article] 15q11.2-13.3 chromatin analysis reveals epigenetic regulation of CHRNA7 with deficiencies in Rett and autism brain (2011)
  • Author(s): Yasui, D
  • Journal Title: Human Molecular Genetics Volume: 22 Issue: 22 Pages: 4311-4321
  • DOI: 10.1093/hmg/ddr357
  • Peer Reviewed
• [Journal Article] Neuron-specific impairment of inter-chromosomal pairing and transcription in a novel model of human 15q-duplication syndrome (2011)
  • Author(s): Meguro-Horike, M.
  • Journal Title: Human Molecular Genetics Volume: 20 Issue: 19 Pages: 3798-3810
  • DOI: 10.1093/hmg/ddr298
  • Peer Reviewed
• [Journal Article] Neuron-specific impairment of inter-chromosomal pairing and transcription in a novel model of human 15q-duplication syndrome. (2011)
  • Author(s): Meguro-Horike M.
  • Journal Title: Human Molecular Genetics Volume: 20 Pages: 3798-3810
  • NAID: 40019439541
  • Peer Reviewed
• [Journal Article] 15q11.2-13.3 chromatin analysis reveals epigenetic regulation of CHRNA7 with deficiencies in Rett and autism brain. (2011)
  • Author(s): Yasui D.H.
  • Journal Title: Human Molecular Genetics Volume: 20 Pages: 4311-4323
  • Peer Reviewed
• [Presentation] A noncoding imprinted RNA、MESTIT1 is essential for the repression in cis of KLF14 (2012)
  • Author(s): Horike S.
  • Organizer: 62thAnnual Meeting; The American Society of Human Genetics
  • Place of Presentation: San Francisco, (USA)
  • Year and Date: 2012-11-08
• [Presentation] 広汎性神経発達障害に関連する 15q11-q13 ゲノム刷り込み領域のアレル特異的クロマチンダイナミクスの解析 (2012)
  • Author(s): 堀家慎一
  • Organizer: 日本人類遺伝学会第57回大会,京王プラザホテル
  • Place of Presentation: 東京都
  • Year and Date: 2012-10-26
• [Presentation] 自閉症とエピジェネティクス」応用動物科学セミナー (2012)
  • Author(s): 堀家慎一
  • Organizer: エピジェネティクスの深淵
  • Place of Presentation: 東京大学 弥生講堂一条ホール,東京都
  • Year and Date: 2012-07-20
• [Presentation] PEG1/MEST 遺伝子領域のゲノム刷り込み制御機構の解明 (2012)
  • Author(s): 堀家慎一
  • Organizer: 第6回日本エピジェネティクス研究会年会,学術総合センター
  • Place of Presentation: 東京都
  • Year and Date: 2012-05-14
• [Presentation] 高次遺伝子発現制御機構へのブレイクスルー (2012)
  • Author(s): 堀家慎一
  • Organizer: 日本分子生物学会 第12回春季シンポジウム
  • Place of Presentation: 石和温泉 慶山,山梨県
  • Year and Date: 2012-04-26
• [Presentation] PEG1/MEST遺伝子領域のゲノム刷り込み制御機構の解明 (2012)
  • Author(s): 堀家慎一
  • Organizer: 第6回日本エピジェネティクス研究会年会
  • Place of Presentation: 学術総合センター（東京都）
• [Presentation] 広汎性神経発達障害に関連する15q11-q13ゲノム刷り込み領域のアレル特異的クロマチンダイナミクスの解析 (2012)
  • Author(s): 堀家慎一
  • Organizer: 日本人類遺伝学会第57回大会
  • Place of Presentation: 京王プラザホテル（東京都）
• [Presentation] A noncoding imprinted RNA, MESTIT1 is essential for the repression in cis of KLF14 (2012)
  • Author(s): 堀家慎一
  • Organizer: 62th Annual Meeting; The American Society of Human Genetics
  • Place of Presentation: the Moscone Center(San Francisco, USA)
• [Presentation] Epigenetics of autism spectrum disorders (2011)
  • Author(s): 堀家慎一
  • Organizer: 第21回日本臨床精神神経薬理学会・第41回日本神経精神薬理学会 合同年会
  • Place of Presentation: 東京都
  • Year and Date: 2011-10-27
• [Presentation] Evidence for a hTERT repressor gene on human chromosome 3p21.3 by using chromosome engineering (2011)
  • Author(s): Ohhira T., Abe S., Tanaka H., Notsu T., Horike S., Qi D.L., Fujisaki C., David G., Oshimura M., Kugoh H
  • Organizer: 12th International Congress of Human Genetics and The ASHG 61stAnnual Meeting
  • Place of Presentation: Montreal, (CANADA)
  • Year and Date: 2011-10-14
• [Presentation] Neuron specific impairment of inter-chromosomal pairing and transcription in a novel model of human 15q-duplication syndrome (2011)
  • Author(s): Horike S., Yasui D.H., Powell W., Schroeder D.I., Oshimura M., LaSalle J.M., Meguro-Horike M.
  • Organizer: 12thInternational Congress of Human Genetics and The ASHG 61st Annual Meeting
  • Place of Presentation: Montreal, (CANADA)
  • Year and Date: 2011-10-12
• [Presentation] 15q11.2-13.3 chromatin analysis reveals epigenetic regulation of CHRNA7 with deficiencies in Rett and autism brain (2011)
  • Author(s): Yasui D.H., Scoles H.A., Horike S.,Meguro-Horike M., Dunaway K.W., Schroeder D.I., Lasalle J.M.
  • Organizer: 12th International Congress of Human Genetics and The ASHG 61stAnnual Meeting
  • Place of Presentation: Montreal, (CANADA)
  • Year and Date: 2011-10-12
• [Presentation] Neuron specific impairment of inter-chromosomal pairing in MeCP2-depleted neuronal cells (2011)
  • Author(s): Horike S., Yasui D.H., Oshimura M., LaSalle J.M., Meguro-Horike M
  • Organizer: 12th Annual Rett syndrome Symposium
  • Place of Presentation: Leesburg, VA, (USA)
  • Year and Date: 2011-06-26
• [Presentation] ヒト 15q11-q13 領域におけるアレル特異的クロマチン脱凝集の解析 (2011)
  • Author(s): 堀家慎一
  • Organizer: 日本分子生物学会 第11回春季シンポジウム
  • Place of Presentation: 金沢(石川県)
  • Year and Date: 2011-05-25
• [Presentation] Role of PWS IC for paternal allele specific chromatin decondensation at 15q11-q13 (2011)
  • Author(s): Horike S., Powell W., LaSalle J.M., Oshimura M., Meguro-Horike M
  • Organizer: 第5回日本エピジェネティクス研究会年会
  • Place of Presentation: 熊本県
  • Year and Date: 2011-05-20
• [Presentation] 15q11-q13 homologous pairing and transcriptions are impaired in a novel neuronal model of 15q duplication syndrome (2011)
  • Author(s): Meguro-Horike M., Yasui D.H., Powell W., Schroeder D.I., Oshimura M., LaSalle J.M., Horike S
  • Organizer: 第5回日本エピジェネティクス研究会年会
  • Place of Presentation: 熊本県
  • Year and Date: 2011-05-19
• [Presentation] A novel model of human 15q-duplication syndrome: Neuron-specific impairment of inter-chromosomal pairing and transcription (2011)
  • Author(s): Horike S., Yasui D.H., Powell W., LaSalle J.M., Meguro-Horike M.
  • Organizer: Cell Symposia, Autism Spectrum Disorders: From Mechanisms to Therapies
  • Place of Presentation: Arlington, VA (USA)
• [Presentation] Neuron specific impairment of inter-chromosomal pairing and transcription in a novel model of human 15q-duplication syndrome (2011)
  • Author(s): Horike S., Yasui D.H., Powell W., Schroeder D.I., Oshimura M., LaSalle J.M., Meguro-Horike M
  • Organizer: The 32nd NAITO CONFERENCE ON Biological basis of mental functions and disorders
  • Place of Presentation: 山梨県
• [Presentation] Higher order inter-chromosomal association of maternal and paternal alleles of 15q11-q13 (2011)
  • Author(s): Horike S.
  • Organizer: ICC on Genomic Imprinting and Beyond
  • Place of Presentation: Barcelona, (Spain)
• [Presentation] Role of PWS IC for paternal allele specific chromatin decondensation at 15q11-q13 (2011)
  • Author(s): 堀家 慎一
  • Organizer: 第5回日本エピジェネティクス研究会年会
  • Place of Presentation: KKRホテル熊本 (熊本県)
• [Presentation] ヒト15q11-q13領域におけるアレル特異的クロマチン脱凝集の解析 (2011)
  • Author(s): 堀家 慎一
  • Organizer: 日本分子生物学会 第11回春季シンポジウム
  • Place of Presentation: 石川県立音楽堂 (石川県)
• [Presentation] MeCP2とクロマチンダイナミクス (2011)
  • Author(s): 堀家 慎一
  • Organizer: 第53回日本小児神経学会総会(招待講演)
  • Place of Presentation: パシフィコ横浜 (神奈川県)
• [Presentation] Neuron specific impairment of inter-chromosomal pairing in MeCP2-depleted neuronal cells (2011)
  • Author(s): 堀家 慎一
  • Organizer: 12th Annual Rett syndrome Symposium
  • Place of Presentation: Landsdowne Resort and Spa (USA)
• [Presentation] Higher order inter-chromosomal association of maternal and paternal alleles of 15q11-q13 (2011)
  • Author(s): 堀家 慎一
  • Organizer: ICC on Genomic Imprinting and Beyond
  • Place of Presentation: Institut d’Estudis Catalans (Spain)
• [Presentation] Neuron specific impairment of inter-chromosomal pairing and transcription in a novel model of human 15q-duplication syndrome (2011)
  • Author(s): 堀家 慎一
  • Organizer: 12th International Congress of Human Genetics and The ASHG 61st Annual Meeting
  • Place of Presentation: Montreal Convention Centrel (CANADA)
• [Presentation] Neuron specific impairment of inter-chromosomal pairing and transcription in a novel model of human 15q-duplication syndrome (2011)
  • Author(s): 堀家 慎一
  • Organizer: The 32nd NAITO CONFERENCE ON Biological basis of mental functions and disorders(招待講演)
  • Place of Presentation: 八ヶ岳ロイヤルホテル (山梨県)
• [Presentation] Epigenetics of autism spectrum disorders (2011)
  • Author(s): 堀家 慎一
  • Organizer: 第21回日本臨床精神神経薬理学会・第41回日本神経精神薬理学会 合同年会(招待講演)
  • Place of Presentation: 京王プラザホテル (東京都)
• [Presentation] A novel model of human 15q-duplication syndrome: Neuron-specific impairment of inter-chromosomal pairing and transcription (2011)
  • Author(s): 堀家 慎一
  • Organizer: Cell Symposia, Autism Spectrum Disorders: From Mechanisms to Therapies
  • Place of Presentation: Sheraton National Hotel (USA)
• [Presentation] Neuron-specific inter-chromosomal pairing and transcription at the GABAR subunit genes cluster (2011)
  • Author(s): 堀家 慎一
  • Organizer: The 34th Annual Meeting of the Molecular Biology Society of Japan
  • Place of Presentation: パシフィコ横浜 (神奈川県)
• [Presentation] 高次遺伝子発現制御機構へのブレイクスルー
  • Author(s): 堀家慎一
  • Organizer: 日本分子生物学会 第12回春季シンポジウム
  • Place of Presentation: 石和温泉慶山（山梨県）
  • Invited
• [Presentation] 自閉症とエピジェネティクス
  • Author(s): 堀家慎一
  • Organizer: 応用動物科学セミナー「エピジェネティクスの深淵」
  • Place of Presentation: 東京大学 弥生講堂一条ホール（東京）
  • Invited