Regulation of nuclear architecture of neuronal cells by nuclear matrix protein SP120
Project/Area Number |
23710216
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Genome biology
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Research Institution | Okayama University |
Principal Investigator |
MIYAJI Mari 岡山大学, 大学院・医歯薬学総合研究科, 助教 (50349255)
|
Project Period (FY) |
2011 – 2012
|
Project Status |
Completed (Fiscal Year 2012)
|
Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
Keywords | 神経細胞 / 核内構造 / AT-rich DNA / 核マトリクス / repeat配列 / エピゲノム / 遺伝子発現制御 / ゲノム環境 / 核構造 / 終末分化 / 反復配列 / 核マトリックス |
Research Abstract |
Several neuron-specific genes placed in the AT-rich genomic environment are regulated by DNA topoisomerase (topo) IIβ. We have identified hnRNP U/SAF-A/SP120 as a protein associated with topo IIβ in differentiating cerebellar neurons. SP120 is a multifunctional nuclear protein that directly binds to DNA and RNA. SP120 specifically and cooperatively binds AT-rich matrix attachment regions (MAR). It was the reported that the N-terminal domain termed SAF-box was required for DNA binding and the C-terminal domain called RGG-box was essential for the interaction with RNA. We showed that a C-terminal domain enriched with Arg-Gly (RG domain) encompassing the RGG-box was important for the MAR-specific binding activity.
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Report
(3 results)
Research Products
(27 results)