Epigenetic regulation of geneexpressions via methylation of histone tail during tissue regeneration
| Project/Area Number |
23710217
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Genome biology
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| Research Institution | Okayama University (2012)
The University of Tokushima (2011) |
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Principal Investigator |
BANDO Tetsuya 岡山大学, 大学院・医歯薬学総合研究科, 助教 (60423422)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | エピゲノム / 器官再生 / 脱分化 / エピジェネティクス / 再生 / ヒストンH3K27メチル化 / ポリコーム |
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| Research Abstract |
Hemimetabolous insects, such as cricket Gryllus bimaculatus, have remarkable regenerative capacity. When cricket nymph loses a part of leg, the lost part of leg is regenerated from blastema, which is a population of dedifferentiated proliferating cells. In the blastema cells, expressions of several epigenetic protein genes including Enhancer of zeste(E(z)), which encodes methyltransferase on histone H3 27th lysine residue (H3K27), and Utx, which encodes demethylase for methylated histone H3K27, were upregulated compared with differentiated cells. We performed RNAi against E(z)a n d Utx. Morphologies of regeneration legs of E(z)(RNAi) and Utx(RNAi) cricket nymphs were abnormal. Several leg patterning genes were ectopically expressed in regeneration leg of E(z)(RNAi) cricket and were diminished in Utx(RNAi) cricket. Methylation of histone H3K27 was diminished and increased in E(z)(RNAi) and Utx(RNAi) crickets, respectively, suggesting that epigenetic regulation of leg patterning genes expressions via methylation of histone H3K27 is involved in repatterning process during leg regeneration.
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Report
(3 results)
Research Products
(39 results)
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[Journal Article] Non-transgenic genome modifications in a hemimetabolous insect using zinc-finger and TAL effector nucleases.2012
Author(s)
Watanabe T, Ochiai H, Sakuma T, Horch HW, Hamaguchi N, Nakamura T, Bando T, Ohuchi H, Yamamoto T, Noji S, Mito T.
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Journal Title
Nature Communications
Volume: 3
Issue: 1
Pages: 1017-1017
DOI
Related Report
Peer Reviewed
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