Comprehensive analysis of cell-surface antigen presenting proteins

• Project/Area Number: 23710218
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Genome biology
• Research Institution: Keio University
• Principal Investigator: MASUDA Takeshi 慶應義塾大学, 政策・メディア研究科, 特任助教 (70383940)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 細胞表面抗原 / ショットガンプロテオミクス / 膜タンパク質 / 相間移動可容化剤 / リン酸化プロテオミクス / 細胞分画 / 相間移動可溶化剤 / プロテオーム

Research Abstract

The expression patterns of cell-surface presenting proteins are different depending on each cell type. This information has been used for identification of cell types, the typing of cancer stem cells and so on. In this study, to profile the patterns of se

Research Products

• [Journal Article] In-depth Membrane Proteomics Study of Breast Cancer Tissues for the Generation of a Chromosome-based Protein List (2013)
  • Author(s): Satoshi Muraoka, Hideaki Kume, Jun Adachi, Takashi Shiromizu, Shio Watanabe, Takeshi Masuda, Yasushi Ishihama, Takeshi Tomonaga
  • Journal Title: Journal of Proteome Research Volume: V12, No.1 Pages: 208-213
  • Peer Reviewed
• [Journal Article] Nestin Protein Is Phosphorylated in Adult Neural Stem/Progenitor Cells and Not Endothelial Progenitor Cells (2012)
  • Author(s): Jun Namiki, Sayuri Suzuki, Takeshi Masuda, Yasushi Ishihama, Hideyuki Okano
  • Journal Title: Stem Cells International Volume: 2012 Pages: 430138-430138
  • DOI: 10.1155/2012/430138
  • Peer Reviewed
• [Journal Article] Construction of a Microscale Phosphoproteomics System with High Sensitivity (2012)
  • Author(s): Takeshi Masuda, Yasushi Ishihama
  • Journal Title: BUNSEKI KAGAKU Volume: V61, No.6 Pages: 459-467
  • NAID: 10030436721
  • Peer Reviewed
• [Journal Article] Nematode-specific tRNAs that decode an alternative genetic code for leucine (2012)
  • Author(s): Kiyofumi Hamashima, Kousuke Fujishima, Takeshi Masuda, Junichi Sugahara, Masaru Tomita, Akio Kanai
  • Journal Title: Nucleic Acids Research Volume: V40, No.8 Pages: 3653-3662
  • Peer Reviewed
• [Journal Article] Microscale phosphoproteome analysis of 10000 cells from human cancer cell lines (2011)
  • Author(s): Takeshi Masuda, Naoyuki Sugiyama, Masaru Tomita, Yasushi Ishihama
  • Journal Title: Analytical Chemistry Volume: V83, No.20 Pages: 7698-7703
  • Peer Reviewed
• [Journal Article] Micro-scale phosphoproteome analysis of 10, 000 cells from human cancer cell lines (2011)
  • Author(s): T. Masuda, N. Sugiyama, M. Tomita, and Y. Ishihama
  • Journal Title: Anal Chem Volume: 83 Issue: 20 Pages: 7698-703
  • DOI: 10.1021/ac201093g
  • Peer Reviewed
• [Presentation] Bernhard Φ. Palsson,Martin Robert, Metabolic response of Escherichia coli adapted to growth on a glycerol minimum medium (2012)
  • Author(s): Kian-Kai Cheng, Baek-Seok Lee, Takeshi Masuda, Junko Fujikura, Takuro Ito, Kazutaka Ikeda, Jiyang Dong, Tomoyoshi Soga, Masaru Tomita
  • Organizer: Foundations of Systems Biology in Engineering 2012
  • Place of Presentation: Tsuruoka
  • Year and Date: 2012-10-22
• [Presentation] Development of a subcellular fractionation protocol using phase transfer surfactants (2012)
  • Author(s): Takeshi Masuda, Naoyuki Sugiyama, Masaru Tomita, Yasushi Ishihama
  • Organizer: 11th Human proteome organization Annual World Congress
  • Place of Presentation: Boston
  • Year and Date: 2012-09-11
• [Presentation] 相間移動可溶化法を用いたショットガンプロテオーム解析 (2012)
  • Author(s): 増田豪
  • Organizer: 第10回北里疾患プロテオーム研究会
  • Place of Presentation: 北里大学
  • Year and Date: 2012-08-23
• [Presentation] ショットガンプロテオミクスのための相間移動可溶化法の開発 (2012)
  • Author(s): 増田豪
  • Organizer: 日本プロテオーム機構 第10回大会
  • Place of Presentation: 東京
  • Year and Date: 2012-07-26
• [Presentation] 相間移動可溶化剤を用いた新規細胞分画法の開発と応用 (2012)
  • Author(s): 増田豪, 杉山直幸, 冨田勝, 石濱泰
  • Organizer: 日本プロテオーム機構第10回大会
  • Place of Presentation: 東京
  • Year and Date: 2012-07-26
• [Presentation] Highly sensitive LC-MS/MS analysis of phosphorylated peptides in minimal number of cells (2012)
  • Author(s): Takeshi Masuda, Yasuyuki Igarashi, Masaru Tomita, Jun Namiki, Naoyuki Sugiyama, Yasushi Ishihama
  • Organizer: 60th ASMS Conference on Mass Spectrometry
  • Place of Presentation: Vancouver
  • Year and Date: 2012-05-24
• [Presentation] 超高熱性アーキアPyrococcus furiosusにおける3タイプのRNAリガーゼと試験管内tRNA断片連結再構成系の構築 (2012)
  • Author(s): 佐藤朝子, 増田豪, 冨田勝, 伊藤隆, 金井昭夫
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
• [Presentation] nev-tRNA: A novel nematode-specific tRNA that decodes an alternative genetic code for leucine (2012)
  • Author(s): Kiyofumi Hamashima, Kosuke Fujishima, Takeshi Masuda, Junichi Sugahara, Masaru Tomita, Akio Kanai
  • Organizer: The 17th Annual Meeting of the RNA Society
  • Place of Presentation: Michigan
• [Presentation] Biochemical Characterization of Three RNA Ligases from the Hyperthermophilic Archaeon Pyrococcus furiosus (2012)
  • Author(s): Asako Sato, Takeshi Masuda, Masaru Tomita, Takashi Itoh, Akio Kanai
  • Organizer: The 17th Annual Meeting of the RNA Society
  • Place of Presentation: Michigan
• [Presentation] Metabolic response of Escherichia coli adapted to growth on a glycerol minimum medium (2012)
  • Author(s): Kian-Kai Cheng, Baek-Seok Lee, Takeshi Masuda, Junko Fujikura, Takuro Ito, Kazutaka Ikeda, Jiyang Dong, Tomoyoshi Soga, Masaru Tomita, Bernhard Palsson, Martin Robert
  • Organizer: Foundations of Systems Biology in Engineering 2012
  • Place of Presentation: 山形
• [Presentation] Development of a subcellular fractionation protocol using phase transfer surfactants (2012)
  • Author(s): Takeshi Masuda, Naoyuki Sugiyama, Masaru Tomita, Yasushi Ishihama
  • Organizer: 11th Human proteome organization Annual World Congress
  • Place of Presentation: ボストン
• [Presentation] 相間移動可溶化法を用いたショットガンプロテオーム解析 (2012)
  • Author(s): 増田豪
  • Organizer: 第10回北里疾患プロテオーム研究会
  • Place of Presentation: 神奈川
  • Invited
• [Presentation] 相間移動可溶化剤を用いた新規細胞分画法の開発と応用 (2012)
  • Author(s): 増田豪, 杉山直幸, 冨田勝, 石濱泰
  • Organizer: 日本プロテオーム機構 第10回大会
  • Place of Presentation: 東京
• [Presentation] nev-tRNA: A novel nematode-specific tRNA that decodes an alternative genetic code for leucine (2012)
  • Author(s): Kiyofumi Hamashima, Kosuke Fujishima, Takeshi Masuda, Junichi Sugahara, Masaru Tomita, Akio Kanai
  • Organizer: The 17th Annual Meeting of the RNA Society
  • Place of Presentation: アナーバー
• [Presentation] Biochemical Characterization of Three RNA Ligases from the Hyperthermophilic Archaeon Pyrococcus furiosus (2012)
  • Author(s): Asako Sato, Takeshi Masuda, Masaru Tomita, Takashi Itoh, Akio Kanai
  • Organizer: The 17th Annual Meeting of the RNA Society
  • Place of Presentation: アナーバー
• [Presentation] Highly sensitive LC-MS/MS analysis of phosphorylated peptides in minimal number of cells (2012)
  • Author(s): Takeshi Masuda, Yasuyuki Igarashi, Masaru Tomita, Jun Namiki, Naoyuki Sugiyama, Yasushi Ishihama
  • Organizer: 60th ASMS Conference on Mass Spectrometry
  • Place of Presentation: バンクーバー
• [Presentation] 高感度リン酸化プロテオーム解析システムの開発 (2011)
  • Author(s): 増田豪, 五十嵐康之, 冨田勝, 並木淳, 杉山直幸, 石濱泰
  • Organizer: 日本プロテオーム機構 第9回大会
  • Place of Presentation: 新潟
  • Year and Date: 2011-07-29
• [Presentation] 微量組織試料に対する定量的リン酸化プロテオーム解析法の確立 (2011)
  • Author(s): 若林真樹, 塚原麻衣, 吉原宏樹, 増田豪, 石濱泰
  • Organizer: 日本プロテオーム機構 第9回大会
  • Place of Presentation: 新潟
  • Year and Date: 2011-07-29
• [Presentation] タンパク質の抽出と分画 (2011)
  • Author(s): 増田豪, 杉山直幸, 石濱泰
  • Organizer: 日本プロテオーム機構第9回大会
  • Place of Presentation: 新潟
  • Year and Date: 2011-07-28
• [Presentation] nev-tRNA: A novel non-canonical type of tRNA in nematodes decodes an alternative genetic code for leucine (2011)
  • Author(s): Kiyofumi Hamashima, Kosuke Fujishima, Takeshi Masuda, Junichi Sugahara, Masaru Tomita, Akio Kanai
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
• [Presentation] 高感度LC-MS/MSシステムを用いた極微量組織試料のプロテオーム解析技術の確立 (2011)
  • Author(s): 若林真樹, 増田豪, 石濱泰
  • Organizer: 第22回クロマトグラフィー科学会議
  • Place of Presentation: 仙台
• [Presentation] 超好熱性アーキアPyrococcus furiosus における3種のRNA ligaseの性状解析 (2011)
  • Author(s): 金井昭夫, 竹末可奈子, 佐藤朝子, 増田豪, 冨田 勝
  • Organizer: 第84回 日本生化学会大会
  • Place of Presentation: 京都
• [Presentation] Metabolic shifts under the stringent response in Escherichia coli (2011)
  • Author(s): Natsumi Saito, Kenji Nakahigashi, Takeshi Masuda, Tomoyoshi Soga, Masaru Tomita
  • Organizer: 7th International Conference of the Metabolomics Society
  • Place of Presentation: Cairns, Australia
• [Presentation] Highly Sensitive Phosphoproteome Analysis of a Minute Number of Cells (2011)
  • Author(s): Takeshi Masuda, Yasuyuki Igarashi, Masaru Tomtia, Jun Namiki, Yasushi Ishihama
  • Organizer: 59th ASMS Conference on Mass Spectrometry
  • Place of Presentation: Denver
• [Presentation] 高感度LC-MS/MSシステムを用いた極微量組織試料のプロテオーム解析技術の確立 (2011)
  • Author(s): 若林真樹
  • Organizer: 第22回クロマトグラフィー科学会議
  • Place of Presentation: 東北大学(仙台市)
• [Presentation] タンパク質の抽出と分画 (2011)
  • Author(s): 増田豪
  • Organizer: 日本プロテオーム機構 第9回大会(招待講演)
  • Place of Presentation: 朱鷺メッセ(新潟市)
• [Presentation] 高感度リン酸化プロテオーム解析システムの開発 (2011)
  • Author(s): 増田豪
  • Organizer: 日本プロテオーム機構 第9回大会
  • Place of Presentation: 朱鷺メッセ(新潟市)
• [Presentation] 微量組織試料に対する定量的リン酸化プロテオーム解析法の確立 (2011)
  • Author(s): 若林真樹
  • Organizer: 日本プロテオーム機構 第9回大会
  • Place of Presentation: 朱鷺メッセ(新潟市)
• [Presentation] Highly Sensitive Phosphoproteome Analysis of a Minute Number of Cells (2011)
  • Author(s): Takeshi Masuda
  • Organizer: 59th ASMS Conference on Mass Spectrometry
  • Place of Presentation: Colorado Convention Center (Denver)
• [Remarks] 奨励賞、日本プロテオーム学会、2012年。
• [Remarks] 2011 JHUPO Best Poster Award、高感度リン酸化プロテオーム解析システムの開発、日本プロテオーム学会、2011年。
• [Patent(Industrial Property Rights)] 獣毛繊維の鑑別方法 (2013)
  • Inventor(s): 増田豪
  • Industrial Property Rights Holder: 一般社団法人ニッセンケン評価センター
  • Filing Date: 2013-03-15
• [Patent(Industrial Property Rights)] エチレングリコール及び界面活性剤を含む溶液を用いた細胞分画法 (2012)
  • Inventor(s): 増田豪, 杉山直幸
  • Industrial Property Rights Holder: 学校法人 慶應義塾
  • Filing Date: 2012-08-22
• [Patent(Industrial Property Rights)] エチレングリコール及び界面活性剤を含む溶液を用いた細胞分画法 (2012)
  • Inventor(s): 増田豪, 杉山直幸
  • Industrial Property Rights Holder: 学校法人 慶應義塾
  • Industrial Property Rights Type: 特許
  • Filing Date: 2012-08-22