Functional Involvement of Tudor in the piRNA Processing Pathway in Drosophila Germlines

• Project/Area Number: 23710219
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Genome biology
• Research Institution: Keio University
• Principal Investigator: NISHIDA Kazumichi 慶應義塾大学, 医学部, 特任助教 (10598436)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: piRNA生合成 / PIWIタンパク質 / TUDORドメインタンパク質 / 生殖細胞

Research Abstract

We discovered that immunopurified BmTud complexes from BmN4 contain BmSpn-E and Siwi. Depletion of BmSpn-E also caused Siwi to be loaded with less piRNAs than that in naive BmN4 cells. We discovered that Siwi, in a form associated with BmSpn-E, is not sig

Research Products

• [Journal Article] Gender-specific hierarchy in nuage localization of PIWI-interacting RNA factors in Drosophila (2011)
  • Author(s): Akihiro Nagao, Kaoru Sato, Kazumichi M. Nishida, Haruhiko Siomi and Mikiko C. Siomi.
  • Journal Title: frontiers in GENETICS Volume: 2 Pages: 1-9
  • Peer Reviewed
• [Presentation] Functional involvement of BmSpn-E in the piRNA biogenesis in silkworm germ line BmN4 cells (2013)
  • Author(s): 西田 知訓
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 福岡 福岡国際会議場・マリンメッセ福岡
  • Year and Date: 2013-12-12
• [Presentation] piRNA biogenesis in silkworm germ line BmN4 cells (2013)
  • Author(s): Kazumichi Nishida
  • Organizer: Keystone Symposia
  • Place of Presentation: Whistler, British Columbia Canada
  • Year and Date: 2013-03-20
• [Presentation] カイコ生殖細胞由来BmN4を用いたpiRNA生合成経路におけるBmSpn-Eの機能解析 (2012)
  • Author(s): 西田知訓
  • Organizer: 分子生物学学会
  • Place of Presentation: マリンメッセ福岡
• [Presentation] piRNA biogenesis in silkworm germ line BmN4 cells (2012)
  • Author(s): Kazumichi M. Nishida
  • Organizer: Keystone Symposia
  • Place of Presentation: Vancouver, British Columbia, Canada
• [Presentation] piRNA processing in silkworm germline cell BmN4 (2011)
  • Author(s): Kazumichi Nishida
  • Organizer: 第16回RNA Society Meeting
  • Place of Presentation: Kyoto International conference center
  • Year and Date: 2011-06-16
• [Presentation] piRNA biogenesis in silkworm germ cell line BmN4 (2011)
  • Author(s): Kazumichi M. Nishida
  • Organizer: 第16回 RNA Society Meeting
  • Place of Presentation: Kyoto、京都国際会議場
• [Presentation] カイコ卵巣由来生殖細胞BmN4を用いたPIWI-interacting RNA生合成機構の解析 (2011)
  • Author(s): 西田知訓
  • Organizer: RNAフロンティアミーティング2011
  • Place of Presentation: 愛知県大府市、あいち健康プラザ
• [Presentation] カイコ生殖細胞由来BmN4を用いたpiRNA生合成経路の解析 (2011)
  • Author(s): 西田知訓
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜、パシフィコ横浜
• [Remarks]
  • URL: http://www.siomilab.med.keio.ac.jp/
• [Remarks] Siomi Lab Keio University School of Medicine
  • URL: http://www.siomilab.med.keio.ac.jp/
• [Remarks]
  • URL: http://web.sc.itc.keio.ac.jp/dmb/sindex.html