Identification of allele-specific methylation regions involved in congenital genomic imprinting disorders

• Project/Area Number: 23710223
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Medical genome science
• Research Institution: Tohoku University
• Principal Investigator: HIURA Hitoshi 東北大学, 医学(系)研究科(研究院), 助教 (70451523)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 生殖補助医療 / インプリント / 生殖補助医療（ART） / 男性精子 / DNAメチル化 / ゲノムインプリント

Research Abstract

Recently, assisted reproductive technologies (ART) have become available to infertile couples who wish to have children. However, reports of rare imprinting disorders have been increased around the globe with the popularization of assisted reproductive technologies. In this study, to clarify the influence of ART for genomic imprinting and identify the ART-related risk factor(s), we analyzed the DNA methylation status of 22 differentially methylated regions in BWS and SRS patients. We observed a more widespread disruption of genomic imprints after ART. Our data suggest that ART-related risk factor(s) was a process after the fertilization than the gamete formative period.

Research Products

• [Journal Article] Stability of the abnormal imprinting of human induced pluripotent stem cells (2013)
  • Author(s): Hiura H, Toyoda M, Okae H, Sakurai M, Miyauchi N, Sato A, Kiyokawa N, Okita H, Miyagawa Y, Akutsu H, Nishino K, Umezawa A, Arima T
  • Journal Title: BMC Genetics Volume: 14 Issue: 1 Pages: 32-32
  • DOI: 10.1186/1471-2156-14-32
  • Peer Reviewed
• [Journal Article] DNA methylation errors in imprinting disorders and assisted reproductive technologies. (2013)
  • Author(s): Chiba H, Hiura H, Okae H, Miyauchi N, Sato F, Sato A, Arima T.
  • Journal Title: Pediatrics international. Volume: 55 Issue: 5 Pages: 542-549
  • DOI: 10.1111/ped.12185
  • Peer Reviewed
• [Journal Article] Aberrant DNA methylation of imprinted loci in male and female germ cells of infertile couples (2012)
  • Author(s): Arima T,Okae H, Hiura H, Miyauchi N, Sato F, Sato A, Hayashi C
  • Journal Title: DNA METHYLATION INTECH Volume: 29 Pages: 183-192
  • URL: http://www.intechopen.com/books/dna-methylation-from-genomics-to-technology
  • Peer Reviewed
• [Journal Article] High-throughput detection of aberrant imprint methylation in the ovarian cancer by the bisulphite PCR-Luminex method. (2012)
  • Author(s): Hiura H, Okae H, Kobayashi H, Miyauchi N, Sato F, Sato A, Suzuki F, Nagase S, Sugawara J, Nakai K, Yaegashi N, Arima T.
  • Journal Title: BMC Medical Genomics Volume: 5 Issue: 1 Pages: 8-17
  • DOI: 10.1186/1755-8794-5-8
  • Peer Reviewed
• [Journal Article] Characterization of DNA methylation errors in patients with imprinting disorders conceived by assisted reproductive technologies. (2012)
  • Author(s): Hiura H, Okae H, Miyauchi N, Sato F, Sato A , Van De Pette M, John R M, Kagami M, Nakai K, Soejima H, Ogata T, Arima T.
  • Journal Title: Human Reproduction Volume: 27(8) Issue: 8 Pages: 2541-2548
  • DOI: 10.1093/humrep/des197
  • Peer Reviewed
• [Journal Article] Re-investigation and RNA sequencing-based identification of genes with placenta-specific imprinted expression (2012)
  • Author(s): Hiroaki Okae, et al
  • Journal Title: Human Molecular Genetics Volume: 21 Issue: 3 Pages: 548-558
  • DOI: 10.1093/hmg/ddr488
  • Peer Reviewed
• [Journal Article] 生殖補助医療由来の先天性ゲノムインプリンティング異常症 (2012)
  • Author(s): 有馬隆博、樋浦仁、岡江寛明
  • Journal Title: 日本生殖内分泌学会雑誌 Japanese Journal of Reproductive Endocrinology Volume: 17 Pages: 54-58
  • NAID: 40019434316
• [Journal Article] Aberrant DNA Methylation of Imprinted (2012)
  • Author(s): Arima T, Okae H, Hiura H et al
  • Journal Title: INTECH Volume: 29 Pages: 183-192
  • Peer Reviewed
• [Journal Article] Role for piRNAs and non-coding RNA in de novo DNA methylation of the imprinted mouse Rasgrf1 locus (2011)
  • Author(s): Watanabe, T., Tomizawa, S., Mitsuya, K., Totoki, Y., Yamamoto, Y., Kuramochi-Miyagawa, S., Iida, N., Hoki, Y., Murphy, P.J., Toyoda, A., Gotoh, K., Hiura, H., Arima, T., Fujiyama, A., Sado, T., Shibata, T., Nakano, T., Lin, H., Ichiyanagi, K., Soloway, P.D., Sasaki, H
  • Journal Title: Science Volume: 332 Issue: 6031 Pages: 848-52
  • DOI: 10.1126/science.1203919
  • Peer Reviewed
• [Journal Article] Assessing loss of imprint methylation in sperm from subfertile men using novel methylation PCR-Luminex analysis (2011)
  • Author(s): Sato A, Hiura H, Okae H, Miyauchi N, Abe Y, Utsunomiya T, Yaegashi N, Arima T
  • Journal Title: Fertil and Steril Volume: 95 Issue: 1 Pages: 129-134
  • DOI: 10.1016/j.fertnstert.2010.06.076
  • Peer Reviewed
• [Book] DNA METHYLATION (2012)
  • Author(s): Arima T, Okae H, Hiura H, Miyauchi N, Sato F, Sato A, Hayashi C.
  • Publisher: INTECH
• [Book] 産婦人科の実際 (2011)
  • Author(s): 有馬隆博、樋浦仁、岡江寛明、佐藤晶子、宮内尚子、阿部千鶴、林千賀
  • Publisher: 金原出版株式会社
• [Book] 助産雑誌 (2011)
  • Author(s): 有馬隆博、樋浦仁、岡江寛明、佐藤晶子、宮内尚子
  • Publisher: 医学書院
• [Book] 癌と化学療法 (2011)
  • Author(s): 有馬隆博、樋浦仁、岡江寛明、宮内尚子、佐藤芙美
  • Publisher: 癌と化学療法社
• [Book] 卵子学 (2011)
  • Author(s): 有馬隆博、樋浦仁、岡江寛明、佐藤晶子、宮内尚子
  • Publisher: 京都大学学術出版会