Transcription regulation by Transcription factors and Epigenetic factors

• Project/Area Number: 23710224
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Medical genome science
• Research Institution: Osaka University
• Principal Investigator: NIMURA Keisuke 大阪大学, 医学系研究科, 助教 (00462713)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 疾患エピゲノミクス / 転写 / ゲノム / 心臓 / エピジェネティクス

Research Abstract

Human genome has over 20,000 genes. Transcription factors and epigenetic factors including histone modifiers, are pivotal factors for gene expression.Mutation in these genes causes congenital heart diseases. But it was still unclear which genes are regulated by these transcription factors. Here, we identified these transcription factors-binding genomic regions using next-generation sequencer, and the target genes that are regulated by these factors.

Research Products

• [Journal Article] The Transcription Factors Tbx18 and Wt1 Control the Epicardial Epithelial-Mesenchymal Transition through Bi-Directional Regulation of Slug in Murine Primary Epicardial Cells. (2013)
  • Author(s): Takeichi M
  • Journal Title: PLoS One. Volume: 8 Issue: 2 Pages: e57829-e57829
  • DOI: 10.1371/journal.pone.0057829
  • Peer Reviewed
• [Journal Article] Essential role for miR-196a in brown adipogenesis of white fat progenitor cells. (2012)
  • Author(s): Mori M, Nakagami H, Rodriguez-Araujo G, Nimura K, Kaneda Y.
  • Journal Title: PLoS Biol Volume: 10(4) Issue: 4 Pages: e1001314-e1001314
  • DOI: 10.1371/journal.pbio.1001314
  • Peer Reviewed
• [Journal Article] Androgen-regulated transcriptional control of sialyltransferases in prostate cancer cells (2012)
  • Author(s): Nonomura N, Takayama H, et al
  • Journal Title: PLoS One Volume: e31234 Issue: 2 Pages: 1570-1573
  • DOI: 10.1371/journal.pone.0031234
  • Peer Reviewed
• [Journal Article] Androgen-Regulated Transcriptional Control of Sialyltransferases in Prostate Cancer Cells. (2012)
  • Author(s): Hatano K, Miyamoto Y, Mori M, Nimura K, Nakai Y, Nonomura N, Kaneda Y
  • Journal Title: PLos one Volume: 7
  • Peer Reviewed
• [Journal Article] PDGFRalpha-positive cells in bone marrow are mobilized by high mobility group box 1 (HMGB1) to regenerate injured epithelia. (2011)
  • Author(s): Tamai K, Yamazaki T, Chino T, Ishii M, Otsuru S, Kikuchi Y, Iinuma S, Saga K, Nimura K, Shimbo T, Umegaki N, Katayama I, Miyazaki J, Takeda J, McGrath JA, Uitto J, Kaneda Y.
  • Journal Title: Proc Natl Acad Sci U S A. Volume: 108(16) Issue: 16 Pages: 6609-14
  • DOI: 10.1073/pnas.1016753108
• [Journal Article] DNA methyltransferase 3b preferentially associates with condensed chromatin (2011)
  • Author(s): Kashiwagi K., Nimura K., Ura K., Kaneda Y.
  • Journal Title: Nucleic Acids Res Volume: 39 Issue: 3 Pages: 874-888
  • DOI: 10.1093/nar/gkq870
  • Peer Reviewed
• [Journal Article] Immunogene therapy using immunomodulating HVJ-E vector augments anti-tumor effects in murine malignant glioma. (2011)
  • Author(s): Matsuda M, Nimura K, Shimbo T, Hamasaki T, Yamamoto T, Matsumura A, Kaneda Y.
  • Journal Title: J Neurooncol. Volume: 103(1) Issue: 1 Pages: 19-31
  • DOI: 10.1007/s11060-010-0355-x
• [Journal Article] 心臓発生におけるエピジェネティックな転写制御 (2011)
  • Author(s): 二村圭祐
  • Journal Title: 生化学 Volume: 83 Pages: 1043-1047
  • NAID: 10030345715
• [Journal Article] ヒストン修飾による心臓転写因子の機能制御 (2011)
  • Author(s): 二村圭祐(責任著者) 、金田安史
  • Journal Title: Annual Review 循環器2011 Pages: 17-22
• [Journal Article] PDGFRα-positive cells in bone marrow are mobilized by high mobility group box 1 (HMGB1) to regenerate injured epithelia. (2011)
  • Author(s): Tamai K, Yamazaki T, Chino T, Ishii M, Otsuru S, Kikuchi Y, Iinuma S, Saga K, Nimura K, Shimbo T, Umegaki N, Katayama I, Miyazaki J, Takeda J, McGrath J A, Uitto J, Kaneda Y
  • Journal Title: Proc Natl Acad Sci U S A Volume: 108
  • Peer Reviewed
• [Journal Article] DNA methyltransferase 3b preferentially associates with condensed chromatin. (2011)
  • Author(s): Kashiwagi K, Nimura K, Ura K, Kaneda Y
  • Journal Title: Nucleic Acids Research Volume: 39
  • Peer Reviewed
• [Journal Article] Epigenetic regulation of transcription in heart development (2011)
  • Author(s): Nimura K
  • Journal Title: 生化学 Volume: 83
  • NAID: 10030345715
• [Presentation] 心発生における転写環境の解明 (2013)
  • Author(s): 二村圭祐
  • Organizer: 若手ワークシ ップ@鬼怒川
  • Place of Presentation: 栃木
• [Presentation] 心発生における転写環境の解明 (2013)
  • Author(s): 二村圭祐
  • Organizer: 若手ワークショップ＠鬼怒川
  • Place of Presentation: 栃木
• [Presentation] 心臓発生における転写制御機構 (2012)
  • Author(s): 二村圭祐
  • Organizer: 大阪大学生命機能研究科コロキウム
  • Place of Presentation: 大阪
  • Year and Date: 2012-10-10
• [Presentation] Transcription regulation in heart development (2012)
  • Author(s): 二村圭祐
  • Organizer: 生命医薬情報学連合大会
  • Place of Presentation: 東京
• [Presentation] 心発生における転写環境の解明 (2012)
  • Author(s): 二村圭祐
  • Organizer: 転写代謝システム班会議
  • Place of Presentation: 筑波
• [Presentation] A cardiac transcription factor links chromatin conformation to transcription termination (2012)
  • Author(s): 二村圭祐
  • Organizer: EMBO Conferenece
  • Place of Presentation: ドイツ
• [Presentation] Transcription regulation in heart development (2012)
  • Author(s): 二村圭祐
  • Organizer: 日本分子生物学会
  • Place of Presentation: 福岡
• [Presentation] A cardiac transcription factor links chromatin conformation to transcription termination (2012)
  • Author(s): 二村圭祐
  • Organizer: EMBO Conference
  • Place of Presentation: ドイツ