Evaluation of inflammatory bowel disease by metabolomics and identification of the therapeutic molecule candidates

• Project/Area Number: 23710256
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Living organism molecular science
• Research Institution: Kobe University
• Principal Investigator: NISHIUMI Shin 神戸大学, 医学(系)研究科(研究院), 特命講師 (20514706)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 炎症性腸疾患 / メタボロミクス / 潰瘍性大腸炎 / クローン病

Research Abstract

The aim of this study was to elucidate whether the pathogenesis of inflammatory bowel disease leads to alterations in the levels of low molecular weight metabolites by using metabolomics. As a result, the experiments using colon of sodium dextran sulfate-induced colitis mice, IL10 deficient colitis mice and ulcerative colitis patients found that the level of glutamine in the colon was decreased by the pathogenesis of inflammatory bowel disease. In addition, it was also confirmed that disease activity of ulcerative colitis could be evaluated by metabolite alterations.

Research Products

• [Journal Article] A novel gas chromatography mass spectrometry-based serum diagnostic and assessment approach to ulcerative colitis (2014)
  • Author(s): Kohashi M, Nishiumi S, Ooi M, Yoshie T, Matsubara A, Suzuki M, Hoshi N, Kamikozuru K, Yokoyama Y, Fukunaga K, Nakamura S, Azuma T, Yoshida M
  • Journal Title: Journal of Crohn's & colitis Volume: 未定 Issue: 9 Pages: 1010-1021
  • DOI: 10.1016/j.crohns.2014.01.024
  • Peer Reviewed
• [Journal Article] GC/MS-based profiling of amino acids and TCA cycle-related molecules in ulcerative colitis (2011)
  • Author(s): Ooi M., Nishiumi S., Yoshie T., Shiomi Y., Kohashi M., Fukunaga K., Nakamura S., Matsumoto T., Hatano N., Shinohara M., Irino Y., Takenawa T., Azuma T., Yoshida M.
  • Journal Title: Inflammation Research Volume: 60 Pages: 831-840
  • Peer Reviewed
• [Journal Article] A GCMS-based metabolomic study in mice with colitis induced by dextran sulfate sodium (2011)
  • Author(s): Shiomi Y., Nishiumi S., Ooi M., Hatano N., Shinohara M., Yoshie T., Kondo Y., Furumatsu K., Shiomi H., Kutsumi H., Azuma T., Yoshida M.
  • Journal Title: Inflammatory Bowel Diseases Volume: 17 Issue: 11 Pages: 2261-2274
  • DOI: 10.1002/ibd.21616
  • Peer Reviewed
• [Journal Article] A novel gas chromatography mass spectrometry-based serum diagnostic and assessment approach to ulcerative colitis
  • Author(s): Kohashi M., Nishiumi S., Ooi M., Yoshie T., Matsubara A., Suzuki M., Hoshi N., Kamikozuru K., Yokoyama Y., Fukunaga K., Nakamura S., Azuma T., Yoshida M.
  • Journal Title: Journal of Crohn's & colitis Volume: (in press)
  • Peer Reviewed
• [Presentation] メタボロミクスの消化器疾患診断・治療への応用 (2014)
  • Author(s): 西海信
  • Organizer: 日本薬学会第134年会シンポジウム
  • Place of Presentation: 熊本
• [Presentation] メタボロミクスの消化器疾患診断・治療への応用 (2014)
  • Author(s): 西海 信
  • Organizer: 日本薬学会第134年会
  • Place of Presentation: 熊本
  • Invited
• [Presentation] メタボロミクスの消化器疾患への応用 (2013)
  • Author(s): 西海信
  • Organizer: 第3回日本質量分析学会春季シンポジウム
  • Place of Presentation: 大阪
  • Year and Date: 2013-06-20
• [Presentation] メタボロミクスの消化器疾患への応用 (2013)
  • Author(s): 西海 信
  • Organizer: 第3回日本質量分析学会 春季シンポジウム
  • Place of Presentation: 大阪
  • Invited
• [Presentation] メタボロミクスを用いた炎症性疾患に対する新規治療薬の探索への試み (2012)
  • Author(s): 西海信、和泉自泰、松原惇起、東健、吉田優
  • Organizer: 第37回日本医用マススペクトル学会年会
  • Place of Presentation: 愛知
• [Remarks]
  • URL: http://www.med.kobe-u.ac.jp/gi/index.html
• [Remarks] 神戸大学大学院医学研究科内科学講座消化器内科学分野ホームページ
  • URL: http://www.med.kobe-u.ac.jp/gi/index.html
• [Remarks]
  • URL: http://www.med.kobe-u.ac.jp/gi/index.htm