Development of the method for constructing artificial snoRNAregulating for target-gene expression.

• Project/Area Number: 23710263
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Living organism molecular science
• Research Institution: Fukuoka University
• Principal Investigator: FUKUDA Masatora 福岡大学, 理学部, 助教 (90526691)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 生体分子の化学修飾 / 核小体低分子RNA / RNA修飾 / 機能性RNA / 細胞スクリーニング / 遺伝子発現制御

Research Abstract

The research aim of this study is to develop the strategy for constructing an artificial snoRNA regulating the target-gene expression in the cell. snoRNAs targeting2’-O-methylation to A or G at the start codon of target-mRNA were obtained by the cell-based screening with the snoRNA library. As the results of characterization for selected snoRNAs, these snoRNAs slightly reduced the target-protein expression.

Research Products

• [Journal Article] A strategy for developing a hammerhead ribozyme for selective RNA cleavage depending on substitutional RNA editing (2012)
  • Author(s): Fukuda, M., Kurihara, K., Tanaka, Y., Deshimaru,M.
  • Journal Title: RNA Volume: 18 Pages: 1735-1744
  • Peer Reviewed
• [Journal Article] Construction of dopamine sensors by using fluorescent ribonucleopeptide complexes (2011)
  • Author(s): Liew, F.F., Hasegawa, T., Fukuda, M., Nakata, E., Morii, T.
  • Journal Title: Bioorganic and Medicinal Chemistry Volume: 19 Pages: 4473-4481
  • NAID: 120003367879
  • Peer Reviewed
• [Presentation] 標的遺伝子発現を抑制する核小体低分子RNAの構築 (2012)
  • Author(s): 川本崇仁、弟子丸正伸、福田将虎
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場・マリンメッセ福岡
• [Presentation] 標的遺伝子の発現を抑制する核小体低分子RNAの構築 (2012)
  • Author(s): 川本崇仁、弟子丸正伸、福田将虎
  • Organizer: 第14回RNA学会
  • Place of Presentation: 東北大学百周年記念会館
• [Presentation] RNA編集を特異的に識別するリボザイムの構築 (2011)
  • Author(s): 栗原圭、弟子丸正伸、福田将虎
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-12-13
• [Presentation] セロトニン2C型受容体mRNAの編集におけるADAR1とADAR2の共働的な作用 (2011)
  • Author(s): 田中泰圭、加藤卓、福田将虎、弟子丸正伸
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-12-13
• [Presentation] マウス脳内における2'-O-メチル化がRNA編集に及ぼす影響の解析 (2011)
  • Author(s): 山口彰太、福田将虎、弟子丸正伸
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-12-13
• [Presentation] ハンマーヘッド型リボザイムを基盤としたRNA修飾を識別するリボザイムの構築 (2011)
  • Author(s): 栗原圭、弟子丸正伸、福田将虎
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都国際会議場
  • Year and Date: 2011-09-24
• [Presentation] Construction ofribozymes recognizing specific RNA modifications in HTR2C mRNA (2011)
  • Author(s): Masatora Fukuda, Kei Kurihara,Masanobu Deshimaru
  • Organizer: The 16th Annual Meeting of the RNA Society, The RNA Society of Japan 13th Annual Meeting
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2011-06-16
• [Presentation] Editing pattern of serotonin 2C receptor mRNA depends on the expression level of ADARs (2011)
  • Author(s): Yasuyoshi Tanaka, Suguru Kato, Masatora Fukuda, Masanobu Deshimaru
  • Organizer: The 16th Annual Meeting of the RNA Society, The RNA Society of Japan 13th Annual Meeting
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2011-06-16
• [Presentation] RNA配列上の修飾を識別するリボザイムの構築 (2011)
  • Author(s): 栗原圭、山本諭司、福田将虎、弟子丸正伸
  • Organizer: 平成23年度日本生化学会九州支部例会
  • Place of Presentation: 久留米大学
  • Year and Date: 2011-05-21
• [Presentation] ADAR1の発現量に依存したセロトニン2C型受容体mRNAの編集パターンの変動 (2011)
  • Author(s): 田中泰圭、加藤卓、福田将虎、弟子丸正伸
  • Organizer: 平成23年度日本生化学会九州支部例会
  • Place of Presentation: 久留米大学
  • Year and Date: 2011-05-21
• [Presentation] ADAR2の発現量に依存したセロトニン2C型受容体mRNAの編集パターンの変動 (2011)
  • Author(s): 加藤卓、田中泰圭、福田将虎、弟子丸正伸
  • Organizer: 平成23年度日本生化学会九州支部例会
  • Place of Presentation: 久留米大学
  • Year and Date: 2011-05-21
• [Presentation] Construction of ribozymes recognizing specific RNA modifications in HTR2C mRNA (2011)
  • Author(s): Masatora Fukuda, Kei Kurihara, Masanobu Deshimaru
  • Organizer: The 16th Annual Meeting of the RNA Society, The RNA Society of Japan 13th Annual Meeting
  • Place of Presentation: Kyoto, Japan
• [Presentation] RNA配列上の修飾を識別するリボザイムの構築
  • Author(s): 栗原圭、山本諭司、福田将虎、弟子丸正伸
  • Organizer: 平成２３年度日本生化学会九州支部例会
  • Place of Presentation: 久留米大学
• [Presentation] ADAR1の発現量に依存したセロトニン2C型受容体mRNAの編集パターンの変動
  • Author(s): 田中泰圭、加藤卓、福田将虎、弟子丸正伸
  • Organizer: 平成２３年度日本生化学会九州支部例会
  • Place of Presentation: 久留米大学
• [Presentation] ADAR2の発現量に依存したセロトニン2C型受容体mRNAの編集パターンの変動
  • Author(s): 加藤卓、田中泰圭、福田将虎、弟子丸正伸
  • Organizer: 平成２３年度日本生化学会九州支部例会
  • Place of Presentation: 久留米大学