Development of the method for constructing artificial snoRNAregulating for target-gene expression.
| Project/Area Number |
23710263
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Living organism molecular science
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| Research Institution | Fukuoka University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 生体分子の化学修飾 / 核小体低分子RNA / RNA修飾 / 機能性RNA / 細胞スクリーニング / 遺伝子発現制御 |
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| Research Abstract |
The research aim of this study is to develop the strategy for constructing an artificial snoRNA regulating the target-gene expression in the cell. snoRNAs targeting2’-O-methylation to A or G at the start codon of target-mRNA were obtained by the cell-based screening with the snoRNA library. As the results of characterization for selected snoRNAs, these snoRNAs slightly reduced the target-protein expression.
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Report
(3 results)
Research Products
(17 results)
