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Biosynthetic mechanism of the wobble modified uridines in tRNA

Research Project

Project/Area Number 23710265
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Chemical biology
Research InstitutionThe University of Tokyo

Principal Investigator

SUZUKI Takeo  東京大学, 大学院・工学系研究科, 講師 (90533125)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywords細胞内化学反応 / tRNA / 転写後修飾 / ミトコンドリア / デコーディング / 生合成 / ウォブル位 / ウリジン / LC/MS
Research Abstract

We investigated two human gene products, hGTPBP3 and hMTO1, which modify the wobble uridine of human mitochondrial tRNAs. They localized in mitochondria and interacted each other. Replacement of each of these genes from yeast homologues to human genes did not complement modification activity, and the C-terminal region of hGTPBP3 was suggested to be required for the wobble modification.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (31 results)

All 2013 2012 2011 Other

All Journal Article (17 results) (of which Peer Reviewed: 17 results,  Acknowledgement Compliant: 1 results) Presentation (14 results)

  • [Journal Article] Decoding mechanism of non-universal genetic codes in Loligo bleekeri mitochondria2013

    • Author(s)
      Ohira T, Suzuki T (第2著者), 他5名
    • Journal Title

      J . Biol. Chem

      Volume: 288 Issue: 11 Pages: 7645-7652

    • DOI

      10.1074/jbc.m112.439554

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Crystal structure of a putative methyltransferase SAV1081 from Staphylococcus aureus2013

    • Author(s)
      Kita, S., Tanaka, Y., Hirano, N., Kimura, S., Suzuki, T., Suzuki, T., Yao, M. and Tanaka, I.
    • Journal Title

      Protein & Peptide Letters

      Volume: 20 Issue: 5 Pages: 530-537

    • DOI

      10.2174/0929866511320050006

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Base methylations in the double-stranded RNA by a fused methyltransferase bearing unwinding activity2012

    • Author(s)
      Kimura S., Ikeuchi Y., Kitahara K., Sakaguchi Y., Suzuki T., Suzuki T.
    • Journal Title

      Nucleic Acids Research

      Volume: (掲載確定)(印刷中) Issue: 9 Pages: 4071-4085

    • DOI

      10.1093/nar/gkr1287

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Molecular basis of dihydrouridine formation on tRNA2011

    • Author(s)
      Yu F, Tanaka Y, Yamashita K, Suzuki T (第4著者), 他4名
    • Journal Title

      Proc Natl Acad Sci U S A

      Volume: 108 Issue: 49 Pages: 19593-19598

    • DOI

      10.1073/pnas.1112352108

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Taurine-containing uridine modifications in tRNA anticodons are required to decipher non-universal genetic codes in ascidian mitochondria2011

    • Author(s)
      Suzuki T (第1著者), 他8名
    • Journal Title

      J. Biol. Chem

      Volume: 286 Issue: 41 Pages: 35494

    • DOI

      10.1074/jbc.m111.279810

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Loss of ribosomal RNA modification causes developmental defects in zebrafish2011

    • Author(s)
      Higa-Nakamine S, Suzuki T (第2著者) 他7名
    • Journal Title

      Nucleic Acids Research

      Volume: 40 Issue: 1 Pages: 391-398

    • DOI

      10.1093/nar/gkr700

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Human Mitochondrial tRNAs : Biogenesis, Function, Structural Aspects, and Diseases2011

    • Author(s)
      Suzuki T, Nagao A and Suzuki T
    • Journal Title

      Annu. Rev. Genet

      Volume: 45 Issue: 1 Pages: 299-329

    • DOI

      10.1146/annurev-genet-110410-132531

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Deficit of tRNA(Lys) modification by Cdkall causes the development of type 2 diabetes in mice2011

    • Author(s)
      Wei FY., Suzuki T., Watanabe S., Kimura S., Kaitsuka T., Fujimura A., Matsui H., Atta M., Michiue H., Fontecave M., Yamagata K., Suzuki T., Tomizawa K.
    • Journal Title

      The Journal of Clinical Investigation

      Volume: 121 Issue: 9 Pages: 3598-3608

    • DOI

      10.1172/jci58056

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Actin-binding protein ABP140 is a methyltransferase for 3-methylcytidine at position 32 of tRNAs in Saccharomyces cerevisiae2011

    • Author(s)
      Noma A, Yi S, Katoh T, Takai Y, Suzuki T and Suzuki T
    • Journal Title

      RNA

      Volume: 17 Issue: 6 Pages: 1111-1119

    • DOI

      10.1261/rna.2653411

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Human mitochondrial diseases caused by lack of taurine-modification in mitochondrial tRNAs2011

    • Author(s)
      Suzuki T, Nagao A and Suzuki T
    • Journal Title

      Wiley Interdiscip Rev RNA

      Volume: 2 Issue: 3 Pages: 376-386

    • DOI

      10.1002/wrna.65

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Molecular basis of dihydrouridine formation on tRNA2011

    • Author(s)
      Futao Yu, Yoshikazu Tanaka, Keitaro Yamashita, Takeo Suzuki, Akiyoshi Nakamura, Nagisa Hirano, Tsutomu Suzuki, Min Yao, Isao Tanaka
    • Journal Title

      Proc Natl Acad Sci U S A.

      Volume: 108 Pages: 19593-19598

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Taurine-containing uridine modifications in tRNA anticodons are required to decipher non-universal genetic codes in ascidian mitochondria2011

    • Author(s)
      Takeo Suzuki, Kenjyo Miyauchi, Tsutomu Suzuki, Shin-ichi Yokobori, Naoki Shigi, Akiko Kondow, Nono Takeuchi, Akihiko Yamagishi, and Kimitsuna Watanabe
    • Journal Title

      J. Biol. Chem.

      Volume: 286 Pages: 35494-8

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Loss of ribosomal RNA modification causes developmental defects in zebrafish2011

    • Author(s)
      Sayomi Higa-Nakamine, Takeo Suzuki, Tamayo Uechi, Anirban Chakraborty, Yukari Nakajima, Mikako Nakamura, Naoko Hirano, Tsutomu Suzuki and Naoya Kenmochi
    • Journal Title

      Nucleic Acids Research.

      Volume: 40 Pages: 391-8

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Human Mitochondrial tRNAs: Biogenesis, Function, Structural Aspects, and Diseases.2011

    • Author(s)
      Tsutomu Suzuki, Asuteka Nagao and Takeo Suzuki
    • Journal Title

      Annu. Rev. Genet.

      Volume: 45 Pages: 299-329

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Deficit of Lys-tRNA Modification by Cdkal1 Causes the Development of Type 2 Diabetes in Mice2011

    • Author(s)
      FY Wei, T Suzuki, S Watanabe, S Kimura, T Kaitsuka, A Fujimura, H Matsui, M Atta, H Michiue, M Fontecave, K Yamagata, T Suzuki and K Tomizawa
    • Journal Title

      J. Clin. Invest.

      Volume: 121 Pages: 3598-608

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Actin-binding protein ABP140 is a methyltransferase for 3-methylcytidine at position 32 of tRNAs in Saccharomyces cerevisiae2011

    • Author(s)
      Akiko Noma, Sanghyun Yi, Takayuki Katoh, Yoshimi Takai, Takeo Suzuki and Tsutomu Suzuki
    • Journal Title

      RNA

      Volume: 17 Pages: 1111-1119

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Human mitochondrial diseases caused by lack of taurine-modification in mitochondrial tRNAs2011

    • Author(s)
      Tsutomu Suzuki, Asuteka Nagao and Takeo Suzuki
    • Journal Title

      WIREs RNA

      Volume: 2 Pages: 376-386

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] Characterization of Human Homologues of MnmE and GidA, Required for an xm5U-type Uridine Modification at the Wobble Position of Mitochondrial tRNAs2011

    • Author(s)
      Takeo Suzuki et al
    • Organizer
      RNA2011Kyoto
    • Place of Presentation
      Kyoto, Japan
    • Related Report
      2012 Final Research Report 2011 Research-status Report
  • [Presentation] Base methylations in the double-stranded RNA by a fused methyltransferase bearing unwinding activity in ribosome assembly2011

    • Author(s)
      Satoshi Kimura,Yoshiho Ikeuchi,Kei Kitahara,Yuriko Sakaguchi,Takeo Suzuki,Tsutomu Suzuki
    • Organizer
      MBSJ2011
    • Place of Presentation
      Yokohama, Japan
    • Related Report
      2011 Research-status Report
  • [Presentation] Native chemical structures of Escherichia coli tRNAs determined by mass spectrometric analysis2011

    • Author(s)
      Kenjyo Miyauchi,Satoshi Kimura,Yuriko Sakaguchi,Takeo Suzuki,Tsutomu Suzuki
    • Organizer
      MBSJ2011
    • Place of Presentation
      Yokohama, Japan
    • Related Report
      2011 Research-status Report
  • [Presentation] The third pathway of tRNA export requires the retrograde import of precursor tRNA from cytoplasm to nucleus for its maturation in Saccharomyces cerevisiae2011

    • Author(s)
      Takayuki Ohira,Kenjyo Miyauchi,Yuriko Sakaguchi,Takeo Suzuki,Tsutomu Suzuki
    • Organizer
      MBSJ2011
    • Place of Presentation
      Yokohama, Japan
    • Related Report
      2011 Research-status Report
  • [Presentation] The acetylation of 18S rRNA is an essential modification to synthesize the small subunit of ribosome in Saccharomyces cerevisiae2011

    • Author(s)
      Satoshi Ito,Yu Akamatsu,Akiko Noma,Satoshi Kimura,Yoshiho Ikeuchi,Kenjyo Miyauchi,Yoshikazu Tanaka,Isao Tanaka,Takeo Suzuki,Tsutomu Suzuki
    • Organizer
      MBSJ2011
    • Place of Presentation
      Yokohama, Japan
    • Related Report
      2011 Research-status Report
  • [Presentation] Biochemical characterization of 40S intermediate in the assembly of 50S ribosomal subunit in Escherichia coli2011

    • Author(s)
      Taiga Arai, Satishi Kimura, Yuriko Sakaguchi, Takeo Suzuki and Tsutomu Suzuki
    • Organizer
      MBSJ2011
    • Place of Presentation
      Yokohama, Japan
    • Related Report
      2011 Research-status Report
  • [Presentation] Crystal structure of dihydrouridine synthase in complex with RNA reveals its tRNA recognition and reaction mechanisms2011

    • Author(s)
      Yoshikazu Tanaka, Futao Yu, Keitaro Yamashita, Akiyoshi Nakamura, Takeo Suzuki, Nagisa Hirano, Tsutomu Suzuki, Min Yao, Isao Tanaka
    • Organizer
      RNA2011Kyoto
    • Place of Presentation
      Kyoto, Japan
    • Related Report
      2011 Research-status Report
  • [Presentation] Base Methylations in the Double-stranded RNA by a Fused Methyltransferase Bearing Unwinding Activity2011

    • Author(s)
      Satoshi Kimura, Yoshiho Ikeuchi, Kei Kitahara, Yuriko Sakaguchi, Takeo Suzuki, Tsutomu Suzuki
    • Organizer
      RNA2011Kyoto
    • Place of Presentation
      Kyoto, Japan
    • Related Report
      2011 Research-status Report
  • [Presentation] Necessity of tRNA introns for yeast growth2011

    • Author(s)
      Shunsuke Mori, Toshiya Endo, Takeo Suzuki, Tsutomu Suzuki, Tohru Yoshihisa
    • Organizer
      RNA2011Kyoto
    • Place of Presentation
      Kyoto, Japan
    • Related Report
      2011 Research-status Report
  • [Presentation] In Vivo Reversal of a UV-induced Lesion on Mouse 28S rRNA2011

    • Author(s)
      Atsumi Miyata, Makoto Kitabatake, Takeo Suzuki, Tsutomu Suzuki, Tomoko Sakata, Yohei Kobayashi, Shin Yonehara, Mutsuhito Ohno
    • Organizer
      RNA2011Kyoto
    • Place of Presentation
      Kyoto, Japan
    • Related Report
      2011 Research-status Report
  • [Presentation] Unique characteristics of ascidian mitochondrial tRNAs: Existence of 5-taurinomethyluridine-containing tRNAs involved in deciphering the non-universal genetic codes and an initiator tRNA corresponding to AUG codon2011

    • Author(s)
      Takeo Suzuki, Kenjyo Miyauchi, Shin-ichi Yokobori, Naoki Shigi, Akiko Kondow, Nono Takeuchi, Tsutomu Suzuki, Akihiko Yamagishi, Kimitsuna Watanabe
    • Organizer
      RNA2011Kyoto
    • Place of Presentation
      Kyoto, Japan
    • Related Report
      2011 Research-status Report
  • [Presentation] Alternative pathway of tRNA maturation in Saccharomyces cerevisiae

    • Author(s)
      Takayuki Ohira, Kenjyo Miyauchi, Yuriko Sakaguchi, Takeo Suzuki, Tsutomu Suzuki
    • Organizer
      The 24th tRNA conference
    • Place of Presentation
      Olmue, Chile
    • Related Report
      2012 Annual Research Report
  • [Presentation] The acetylation of 18S rRNA is an essential modification to synthesize the small subunit of ribosome in Saccharomyces cerevisiae

    • Author(s)
      Satoshi Ito, Yu Akamatsu, Akiko Noma, Satoshi Kimura, Yoshiho Ikeuchi, Kenjyo Miyauchi, Yoshikazu Tanaka, Isao Tanaka, Takeo Suzuki, Tsutomu Suzuki
    • Organizer
      The 9th International Conference on Ribosome Synthesis
    • Place of Presentation
      Banff, Canada
    • Related Report
      2012 Annual Research Report
  • [Presentation] Insights into the functional role of the RlmE-mediated Um2552 formation in the late assembly of 50S subunit in Escherichia coli

    • Author(s)
      Taiga Arai, Satoshi Kimura, Yuriko Sakaguchi, Takeo Suzuki, Tsutomu Suzuki
    • Organizer
      The 9th International Conference on Ribosome Synthesis
    • Place of Presentation
      Banff, Canada
    • Related Report
      2012 Annual Research Report

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Published: 2011-08-05   Modified: 2019-07-29  

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