| Project/Area Number |
23710266
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Chemical biology
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
YOSHIDA Suguru 東京医科歯科大学, 生体材料工学研究所, 助教 (10583750)
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| Co-Investigator(Renkei-kenkyūsha) |
INOUYE Satoshi JNC株式会社, 横浜研究所, 主席研究員 (40426622)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | ケミカルバイオロジー / 有機化学 / 分子プローブ / 生物発光 |
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| Research Abstract |
In vivo molecular imaging that enables non-invasive observation of molecule of interest in organisms is a valuable technique to promote life science and drug discovery. In particular, the methods using near-IR luminescence have been aggressively developed in recent years. In order to develop a near-IR bioluminescence system based on coelenterazine (CTZ)-utilizing luciferase, we have established a concise synthetic method of v-CTZ, an analog that is known to show red-shifted light emission applicable to in vivo molecular imaging. The ten-step synthetic route of v-CTZ includes three consecutive regioselective cross-coupling reactions and ring closing metathesis as key reactions, which is suitable for preparing various analogs. We also synthesized C2-CF3-analog of v-CTZ, cf3-v-CTZ, and showed that it also serves as a good substrate for Renilla luciferases with increased stability.
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