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Gene regulation by using an ligand-responsive miRNA pathway

Research Project

Project/Area Number 23710270
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Chemical biology
Research InstitutionOsaka University

Principal Investigator

MURATA Asako  大阪大学, 産業科学研究所, 助教 (50557121)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
KeywordsマイクロRNA / cell-basedセレクション / 低分子化合物 / cell-based セレクション
Research Abstract

This study aimed to explore an artificial miRNA pathway that can be regulated by small-molecule ligands. Ligand-binding sites were created in an endogenous pre-miRNA by partially randomizing the sequence. Construction of the expression vector containing the partially randomized pre-miRNA library and the optimization of conditions for cell-based selection were performed. Moreover, in vitro selection of RNA molecules that bind to a novel naphthyridine derivatives was performed.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (18 results)

All 2014 2013 2012 2011

All Journal Article (5 results) (of which Peer Reviewed: 5 results,  Acknowledgement Compliant: 1 results) Presentation (13 results) (of which Invited: 1 results)

  • [Journal Article] Fluorescent indicator displacement assay of ligands targeting 10 microRNA precursors2013

    • Author(s)
      Murata, A.; Harada, Y.; Fukuzumi, T.; Nakatani, K.
    • Journal Title

      Bioorg. Med. Chem.

      Volume: 21 Issue: 22 Pages: 7101-7106

    • DOI

      10.1016/j.bmc.2013.09.007

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Xanthone derivatives as potential inhibitors of miRNA processing by human Dicer: Targeting secondary structures of pre-miRNA by small molecules.2013

    • Author(s)
      Asako Murata, Takeo Fukuzumi, Shiori Umemoto, Kazuhiko Nakatani
    • Journal Title

      Bioorganic & Medicinal Chemistry Letters

      Volume: 23 Issue: 1 Pages: 252-255

    • DOI

      10.1016/j.bmcl.2012.10.108

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Ligand-inducible formation of RNA pseudoknot2013

    • Author(s)
      Matsumoto, S., Hong, C; Otabe, T.; Murata, A.; Nakatani, K.
    • Journal Title

      Bioorg. Med. Chem. Lett.

      Volume: 23 Issue: 12 Pages: 3539-3541

    • DOI

      10.1016/j.bmcl.2013.04.037

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structure-activity studies on the fluorescent indicator in the displacement assay for the screening of small molecules binding to RNA.2012

    • Author(s)
      Shiori Umemoto, Seongwang Im, Jinhua Zhang, Masaki Hagihara, Asako Murata, Yasue Harada, Takeo Fukuzumi, Takahiro Wazaki, Shin-ichi Sasaoka, and Kazuhiko Nakatani
    • Journal Title

      Chemistry A European Journal

      Volume: 18 Issue: 32 Pages: 9999-10008

    • DOI

      10.1002/chem.201103932

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Structure-activity studies on the fluorescent indicator in the displacement assay for the screening of small molecules binding to RNA2012

    • Author(s)
      Shiori Umemoto, Seongwang Im, Jinhua Zhang, Masaki Hagihara, Asako Murata, Yasue Harada, Takeo Fukuzumi, Takahiro Wazaki, Shin-ichi Sasaoka, and Kazuhiko Nakatani
    • Journal Title

      Chemistry - A European Journal

      Volume: 18

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] RNAの構造・機能を制御する小分子化合物の開発2014

    • Author(s)
      村田亜沙子
    • Organizer
      日本化学会第94春季年会
    • Place of Presentation
      名古屋大学(愛知県)
    • Year and Date
      2014-03-27
    • Related Report
      2013 Final Research Report
  • [Presentation] RNAの構造・機能を制御する小分子化合物の開発2014

    • Author(s)
      村田亜沙子
    • Organizer
      日本化学会第94春季年会
    • Place of Presentation
      名古屋大学(愛知県名古屋市)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] RNA結合性化合物を用いたマイクロRNA 前駆体プロセシングの調節2013

    • Author(s)
      村田亜沙子・福澄岳雄・梅本詩織・神山いづみ・堂野主税・中谷和彦
    • Organizer
      日本化学会第93春季年会
    • Place of Presentation
      立命館大学(滋賀県)
    • Year and Date
      2013-03-23
    • Related Report
      2013 Final Research Report
  • [Presentation] Targeting secondary structures of pre-miRNA by small molecules : Development of potential inhibitors of pre-miRNA processing2013

    • Author(s)
      Murata , A.; Sugai, A.; Fukuzumi, T.; Umemoto, S.; Dohno, C.; Nakatani, K.
    • Organizer
      RNA 2013, the 18th Annual Meeting of the RNA Society
    • Place of Presentation
      Congress Center Davos (Switzerland)
    • Related Report
      2013 Final Research Report
  • [Presentation] Targeting secondary structures of pre-miRNA by small molecules : Development of potential inhibitors of pre-miRNA processing2013

    • Author(s)
      Asako Murata, Ayako Sugai, Takeo Fukuzumi, Shiori Umemoto, Chikara Dohno, Kazuhiko Nakatani
    • Organizer
      RNA 2013, the 18th Annual Meeting of the RNA Society
    • Place of Presentation
      Congress Center Davos (Davos, Switzerland)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Suppression of miR-29a maturation by ligand binding2013

    • Author(s)
      Takahiro Otabe, Asako Murata, Fumie Takei, Kazuhiko Nakatani
    • Organizer
      RNA 2013, the 18th Annual Meeting of the RNA Society
    • Place of Presentation
      Congress Center Davos (Davos, Switzerland)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Translational regulation by ligand-inducible formation of RNA pseudoknot2013

    • Author(s)
      Saki Matsumoto, Changfeng Hong, Asako Murata, Kazuhiko Nakatani
    • Organizer
      RNA 2013, the 18th Annual Meeting of the RNA Society
    • Place of Presentation
      Congress Center Davos (Davos, Switzerland)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Synthesis of the restrained naphthyridine dimer and the exploration for binding RNA by in vitro selection2013

    • Author(s)
      Yue Di, Takahiro Otabe, Jinxing Li, Asako Murata, Kazuhiko Nakatani
    • Organizer
      ISNAC2013
    • Place of Presentation
      神奈川大学(神奈川県横浜市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] RNA結合性化合物を用いたマイクロRNA前駆体プロセシングの調節2013

    • Author(s)
      村田亜沙子・福澄岳雄・梅本詩織・神山いづみ・堂野主税・中谷和彦
    • Organizer
      日本化学会第93春季年会
    • Place of Presentation
      立命館大学(滋賀県)
    • Related Report
      2012 Research-status Report
  • [Presentation] High-throughput Screening of Chemical Libraries for the Discovery of RNA-binding Compounds2012

    • Author(s)
      Asako Murata, Yasue Harada, Takeo Fukuzumi, Shiori Umemoto, Seongwang Im, Masaki Hagihara, Kazuhiko Nakatani
    • Organizer
      RNA 2012, the 17th Annual Meeting of the RNA Society
    • Place of Presentation
      University of Michigan (Ann Arbor, Michigan, USA)
    • Related Report
      2012 Research-status Report
  • [Presentation] 大規模化合物ライブラリーのスクリーニングによる,RNA結合性化合物の探索2012

    • Author(s)
      村田亜沙子, 原田恭枝, 福澄岳雄, 梅本詩織, 任仙光, 萩原正規, 中谷和彦
    • Organizer
      日本化学会第92回春季年会
    • Place of Presentation
      慶応義塾大学(神奈川県)
    • Related Report
      2011 Research-status Report
  • [Presentation] High-throughput Screening of Chemical Libraries for the Discovery of RNA-binding Compounds2011

    • Author(s)
      Asako Murata, Yasue Harada, Takeo Fukuzumi, Shiori Umemoto, Seongwang Im, Masaki Hagihara and Kazuhiko Nakatani
    • Organizer
      RNA 2011
    • Place of Presentation
      国際会議場(京都府)
    • Related Report
      2011 Research-status Report
  • [Presentation] Fluorescent indicator displacement assay for the discovery of RNA-binding ligands2011

    • Author(s)
      Asako Murata, Yasue Harada, Takeo Fukuzumi, Shiori Umemoto, Seongwang Im, Masaki Hagihara and Kazuhiko Nakatani
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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