Elucidation of genetic diversity and genetic management in captive breeding of endangered amphibian species endemic to Ryukyu Islands

• Project/Area Number: 23710282
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Resource conservation science
• Research Institution: Hiroshima University
• Principal Investigator: IGAWA Takeshi 広島大学, 理学(系)研究科(研究院), 特任助教 (00507197)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 集団遺伝学 / 保全遺伝学 / 景観遺伝学 / 両生類 / 絶滅危惧種 / マイクロサテライト / 西南諸島 / GIS / 生息適地モデル / 環境と生物 / 飼育下繁殖

Research Abstract

Genetic diversity of represents the evolutionary history of species and the essential evolutionary potential to respond to changing environments especially for endangered species. Microsatellites are currently the most practical and informative molecular maker for measuring genetic variation within populations. In this study, we developed microsatellite markers for nine endangered amphibian species endemic to Ryukyu Islands in Japan. By using these markers, fine scale population structures and genetic diversities of these species were revealed. These results have contributed to effective conservation and enabled genetic management of ex-situ conservation activities of these endangered amphibians.

Research Products

• [Journal Article] Development and characterization of 14 microsatellite markers for <i>Buergeria japonica</i> (Amphibia, Anura, Rhacophoridae) (2014)
  • Author(s): Shohei Komaki, Takeshi Igawa, Masafumi Nozawa, Si-Min Lin, Shohei Oumi, and Masayuki Sumida
  • Journal Title: Genes & Genetic Systems Volume: 89 Issue: 1 Pages: 35-39
  • DOI: 10.1266/ggs.89.35
  • NAID: 130004137821
  • ISSN: 1341-7568, 1880-5779
  • Peer Reviewed / Open Access
• [Journal Article] Genetic divergences of South and Southeast Asian frogs : a case study of several taxa based on 16S ribosomal RNA gene data with notes on the generic name Fejervarya (2014)
  • Author(s): Mahmudul Hasan, Mohammed Mafizul Islam, Mukhlesur Rahman Khan, and Takeshi Igawa, Mohammad Shafiqul Alam, HonTjong Djong, Nia Kurniawan, Hareesh Joshy, Yong Hoi Sen, Daicus M. Belabut, Atsushi Kurabayashi, Mitsuru Kuramoto, Masayuki Sumida
  • Journal Title: Turkish Journal of Zoology Volume: 38 Pages: 1-22
  • DOI: 10.3906/zoo-1308-36
  • Peer Reviewed / Open Access
• [Journal Article] Genetic divergences of South and Southeast Asian frogs : a case study of several taxa based on 16S ribosomal RNA gene data with notes on the generic name Fejervarya. (2014)
  • Author(s): Mahmudul Hasan, Mohammed Mafizul Islam, Mukhlesur Rahman Khan, and Takeshi Igawa, Mohammad Shafiqul Alam, HonTjong Djong, Nia Kurniawan, Hareesh Joshy, Yong Hoi Sen, Daicus M. Belabut, Atsushi Kurabayashi, Mitsuru Kuramoto, Masayuki Sumida
  • Journal Title: Turkish Journal of Zoology Volume: inpress
  • Peer Reviewed
• [Journal Article] Development and characterization of 14 microsatellite markers for Buergeria japonica (Amphibia, Anura, Rhacophoridae). (2014)
  • Author(s): Shohei Komaki, Takeshi Igawa*, Masafumi Nozawa, Si-Min Lin, Shohei Oumi, and Masayuki Sumida
  • Journal Title: Genes & Genetic Systems Volume: inpress
  • NAID: 130004137821
  • Peer Reviewed
• [Journal Article] Population structure and landscape genetics of two endangered frog species of genus Odorrana: different scenarios on two islands (2013)
  • Author(s): Takeshi Igawa, Shohei Oumi, Seiki Katsuren and Masayuki Sumida
  • Journal Title: Heredity Volume: 110 Issue: 1 Pages: 46-56
  • DOI: 10.1038/hdy.2012.59
  • Peer Reviewed
• [Journal Article] Attempt at Captive Breeding of the Endangered Newt Echinotriton andersoni, from the Central Ryukyus in Japan. (2013)
  • Author(s): Takeshi Igawa, Hirotaka Sugawara, Miyuki Tado, Takuma Nishitani, Atsushi Kurabayashi, Mohammed Islam, Shohei Oumi, Seiki Katsuren, Tamotsu Fujii, Masayuki Sumida
  • Journal Title: Animals Volume: 3 Issue: 3 Pages: 680-692
  • DOI: 10.3390/ani3030680
  • NAID: 120006523852
  • Peer Reviewed
• [Journal Article] Development and characterization of new microsatellite loci in the Otton frog (Babina subaspera) and cross-amplification in a congeneric species, Holst’s frog (B. holsti). (2013)
  • Author(s): Ryosuke Kakehashi, Takeshi Igawa, Noriko Iwai, Etsuko Shoda-Kagaya, and Masayuki Sumida
  • Journal Title: Conservation Genetics Resources Volume: 5 Issue: 4 Pages: 1071-1073
  • DOI: 10.1007/s12686-013-9981-x
  • Peer Reviewed
• [Journal Article] Stable aquaculture of the Japanese lancelet Branchiostoma japonicum for 7 years. (2013)
  • Author(s): Kinya Yasui, Takeshi Igawa, Takao Kaji, and Yasuhisa Henmi
  • Journal Title: Journal of experimental zoology Volume: 320B Issue: 8 Pages: 538-547
  • DOI: 10.1002/jez.b.22540
  • Peer Reviewed
• [Journal Article] Cryptic Anuran Biodiversity in Bangladesh Revealed by Mitochondrial 16S rRNA Gene Sequences (2012)
  • Author(s): Mahmudul Hasan, Mohammed Mafizul Islam, Md Mukhlesur Rahman Khan, Mohammad Shafiqul Alam, Atsushi Kurabayashi, Takeshi Igawa, Mitsuru Kuramoto, and Masayuki Sumida
  • Journal Title: Zoological science Volume: 29(3) Issue: 3 Pages: 162-72
  • DOI: 10.2108/zsj.29.162
  • NAID: 120006473862
  • Peer Reviewed
• [Journal Article] Genomic structure and evolution of multigene families : "flowers" on the human genome (2012)
  • Author(s): Hie Lim Kim, Mineyo Iwase, Takeshi Igawa, Tasuku Nishioka, Satoko Kaneko, Yukako Katsura, Naoyuki Takahata, and Yoko Satta
  • Journal Title: International journal of evolutionary biology Volume: 2012 Pages: 917678-917678
  • DOI: 10.1155/2012/917678
  • NAID: 120006576066
  • Peer Reviewed
• [Journal Article] Isolation and characterization of ten microsatellite loci of endangered Anderson’s crocodile newt, Echinotriton andersoni. (2012)
  • Author(s): Sugawara Hirotaka, Takeshi Igawa, Masashi Yokogawa. Masaru Okuda, Shohei Oumi, Seiki Katsuren, Singo Kaneko, Tetsuya Umino, Yuji Isagi and Masayuki Sumida
  • Journal Title: Conservation Genetics Resources Volume: 7 Issue: 3 Pages: 595-598
  • DOI: 10.1007/s12686-012-9600-2
  • Peer Reviewed
• [Journal Article] Isolation and characterization of twelve microsatellite loci of endangered Ishikawa's frog (Odorrana ishikawae) (2011)
  • Author(s): Takeshi Igawa, Masaru Okuda, Shohei Oumi, Seiki Katsuren, Atsushi Kurabayashi, Tetsuya Umino, and Masayuki Sumida
  • Journal Title: Conservation Genetics Resources Volume: 3(3) Issue: 3 Pages: 421-424
  • DOI: 10.1007/s12686-010-9370-7
  • Peer Reviewed
• [Journal Article] Artificial Production and Natural Breeding of the Endangered Frog Species Odorrana ishikawae, with Special Reference to Fauna Conservation in the Laboratory (2011)
  • Author(s): Masayuki Sumida, Naoki Satou, Natsuhiko Yoshikawa, Atsushi Kurabayashi, Mohammed Mafizul Islam, Takeshi Igawa, Shohei Oumi, Seiki Katsuren, Hidetoshi Ota, Nozomi Shintani, Hiroko Fukuniwa, Naomi Sano, and Tamotsu Fujii
  • Journal Title: Zoological science Volume: 28(11) Issue: 11 Pages: 834-9
  • DOI: 10.2108/zsj.28.834
  • NAID: 120005173618
  • Peer Reviewed
• [Journal Article] 徳之島産イボイモリ(Echinotriton andersoni)の産卵行動の記録 (2011)
  • Author(s): 菅原弘貴, 井川武, 岩井紀子, 山室一樹, 大海昌平, 住田正幸
  • Journal Title: 爬虫両棲類学会報 Volume: 2011 Pages: 115-118
  • NAID: 40019025673
• [Presentation] Conservation genetics of endangered amphibians in Ryukyu Archipelago towards sustainable conservation of the biodiversity hotspot of amphibians (2014)
  • Author(s): Igawa, T.
  • Organizer: International Symposium, "Frontiers in Amphibian Biology : Endangered Species Conservation and Genome Editing"
  • Place of Presentation: Higashi-Hiroshima, Japan
  • Year and Date: 2014-03-28
  • Invited
• [Presentation] Conservation genetics of endangered amphibians in Ryukyu Archipelago towards sustainable conservation of the biodiversity hotspot of amphibians (2014)
  • Author(s): Takeshi Igawa
  • Organizer: International Symposium, “Frontiers in Amphibian Biology: Endangered Species Conservation and Genome Editing
  • Place of Presentation: 東広島市
  • Invited
• [Presentation] An ex situ conservation effort for several endangered and near-threaten amphibian species from the Ryukyu Archipelago, Japan: Captive breeding of nine anuran species and cryopreservation of sperm for two of them (2014)
  • Author(s): Islam, MM., Igawa, T., Kurabayashi, A., Kakehashi, R., Satou, N., Shintani, N., Tado, M., Sugawara, H., Nishitani, T., Uchida, M., Oumi, S., Katsuren, S., Fujii, T., and Sumida, M.
  • Organizer: International Symposium, “Frontiers in Amphibian Biology: Endangered Species Conservation and Genome Editing
  • Place of Presentation: 東広島市
  • Invited
• [Presentation] A case study of fauna conservation in Japan: Current conservation measures of wild populations, captive breeding, genetic diversity, and potential resource materials in the endangered frog species Odorrana ishikawae and O. splendida. (2014)
  • Author(s): Sumida, M., Islam, MM., Igawa, T., Kurabayashi, A., Satou, N., Ukena, K., Oumi, S., Katsuren, S., and Fujii, T
  • Organizer: International Symposium, “Frontiers in Amphibian Biology: Endangered Species Conservation and Genome Editing
  • Place of Presentation: 東広島市
  • Invited
• [Presentation] Translucent frogs created through crossbreeding, and their inheritance and dermal chromatophore structure. (2014)
  • Author(s): Sumida, M., Islam, MM., Igawa, T., Kurabayashi, A., Furukawa, Y., Sano, N., Fujii, T., Yoshizaki, N., and *Nishioka, M.
  • Organizer: International Symposium, “Frontiers in Amphibian Biology: Endangered Species Conservation and Genome Editing"
  • Place of Presentation: 東広島市
• [Presentation] International Xenopus Resource Network and Educational Activities at Institute for Amphibian Biology. (2014)
  • Author(s): Suzuki, A., Kashiwagi, A., Kashiwagi, K., Hanada, H., Takebayashi-Suzuki, K., Tazawa, I., Furuno, N., Kurabayashi, A., Kobayashi, S., Takenaka, J., Tamaki, Y., Igawa, T., Uto, T., Nanba, C., Watanabe, A., Yoshida, H., Shimada, A., and Sumida, M.
  • Organizer: International Symposium, “Frontiers in Amphibian Biology: Endangered Species Conservation and Genome Editing"
  • Place of Presentation: 東広島市
• [Presentation] Inbreeding coefficient and genetic relationship of seven strains of Xenopus tropicalis inferred from genome wide genotyping of 54 microsatellite loci (2014)
  • Author(s): Watanabe, A., Igawa, T., Kashiwagi, A., Suzuki, A., Kurabayashi, A., Fujii, T., and Sumida, M.
  • Organizer: International Symposium, “Frontiers in Amphibian Biology: Endangered Species Conservation and Genome Editing"
  • Place of Presentation: 東広島市
• [Presentation] Molecular cytogenetic studies on the process of genomic and chromosomal evolution in X. laevis after WGD and the origin and evolution of sex chromosomes in anuran species (2014)
  • Author(s): Uno, Y., Nishida, C., Takagi, C., Igawa, T., Ueno, N., Sumida, M., and Matsuda, Y.
  • Organizer: International Symposium, “Frontiers in Amphibian Biology: Endangered Species Conservation and Genome Editing"
  • Place of Presentation: 東広島市
• [Presentation] Eco-evo-devo study of phenotypic plasticity in the Hokkaido salamander (Hynobius retardatus). (2014)
  • Author(s): Matsunami, M., Kishida, O., Kitano, J., Nozawa, M., Igawa, T., Michimae, H., Miura, T., and Nishimura, K.
  • Organizer: International Symposium, “Frontiers in Amphibian Biology: Endangered Species Conservation and Genome Editing"
  • Place of Presentation: 東広島市
• [Presentation] Genetic population structure of an endangered frog species, Babina holsti. (2014)
  • Author(s): Kakehashi, R., Igawa, T., and Sumida, M.
  • Organizer: International Symposium, “Frontiers in Amphibian Biology: Endangered Species Conservation and Genome Editing"
  • Place of Presentation: 東広島市
• [Presentation] New insights into evolutionary history among Asian pond frog species. (2014)
  • Author(s): Komaki, S., Igawa, T., Tojo, K., Kurabayashi, A., Lin, S., Min, M., and Sumida, M.
  • Organizer: International Symposium, “Frontiers in Amphibian Biology: Endangered Species Conservation and Genome Editing"
  • Place of Presentation: 東広島市
• [Presentation] Development and characterization of 27 new microsatellite markers of Indian Bullfrog, Hoplobatrachus tigerinus (Daudin, 1802) and its congeneric species. (2014)
  • Author(s): Sultana, N., Igawa, T., Nozawa, M., Islam MM., Khan, MR., Hasan, M., Alam, MS., and Sumida, M.
  • Organizer: International Symposium, “Frontiers in Amphibian Biology: Endangered Species Conservation and Genome Editing"
  • Place of Presentation: 東広島市
• [Presentation] Evolutionary relationships and postmating isolation among Fejervarya species from Lesser Sunda, Indonesia and other Asian countries revealed by mtDNA Cyt b gene sequences and crossing experiments. (2014)
  • Author(s): Hasan, M., Kurniawan, N., Soewondo, A., Islam, MM., Igawa, T., Nalley, WMM., Matsui, M., and Sumida, M.
  • Organizer: International Symposium, “Frontiers in Amphibian Biology: Endangered Species Conservation and Genome Editing"
  • Place of Presentation: 東広島市
• [Presentation] Ion Torrent PGMを用いたハナサキガエルにおけるマイクロサテライト遺伝子座の単離と集団構造解析(口頭発表) (2013)
  • Author(s): 井川武・長岡麻衣・野澤昌文・小巻翔平・藤井保・住田正幸
  • Organizer: 日本爬虫両生類学会第52回大会
  • Place of Presentation: 札幌
  • Year and Date: 2013-11-02
• [Presentation] Ion PGMを利用した両生類3種におけるマイクロサテライトマーカー開発 (2013)
  • Author(s): 井川武・小巻翔平・Nasrin Sultana・長岡麻衣・野澤昌文・Islam Mohammed Mafizul・大海昌平・住田正幸
  • Organizer: NGS現場の会第三回研究会
  • Place of Presentation: 神戸
  • Year and Date: 2013-09-04
• [Presentation] Ion PGMを利用した両生類3種におけるマイクロサテライトマーカー開発 (2013)
  • Author(s): 井川武、小巻翔平、Nasrin Sultana、長岡麻衣、野澤昌文、Islam Mohammed Mafizul、大海昌平、住田正幸
  • Organizer: NGS現場の会第三回研究会
  • Place of Presentation: 神戸市
• [Presentation] 両生類絶滅危惧種の飼育下繁殖による保全の試み (2013)
  • Author(s): 住田正幸、井川武、イスラム マフィズル、倉林敦、アラム シャフィクル、掛橋竜祐、田戸美雪、新谷望、菅原弘貴、西谷琢磨、大海昌平、勝連盛輝、藤井保
  • Organizer: 日本遺伝学会第85回大会
  • Place of Presentation: 横浜市
• [Presentation] 絶滅危惧種イボイモリの遺伝的多様性と飼育下繁殖 (2013)
  • Author(s): 住田正幸、井川武、菅原弘貴、田戸美雪、西谷琢磨、倉林敦、Mafizul Islam、大海昌平、勝連盛輝、藤井保
  • Organizer: 日本動物学会第84回大会
  • Place of Presentation: 岡山市
• [Presentation] 絶滅危惧種ホルストガエルにおける遺伝的集団構造 (2013)
  • Author(s): 掛橋竜祐・井川武・住田正幸
  • Organizer: 日本爬虫両生類学会第52回大会
  • Place of Presentation: 札幌市
• [Presentation] Ion Torrent PGMを用いたハナサキガエルにおけるマイクロサテライト遺伝子座の単離と集団構造解析 (2013)
  • Author(s): 井川武・長岡麻衣・野澤昌文・小巻翔平・藤井保・住田正幸
  • Organizer: 日本爬虫両生類学会第52回大会
  • Place of Presentation: 札幌市
• [Presentation] 両生類モデル動物ネッタイツメガエル6系統における近交度及び遺伝的関係の解明 (2013)
  • Author(s): 渡辺愛・井川武・鈴木厚・柏木昭彦・倉林敦・藤井保・住田正幸
  • Organizer: 日本爬虫両生類学会第52回大会
  • Place of Presentation: 札幌市
• [Presentation] Evolutionary relationships and postmating isolation among Fejervarya species from Lesser Sunda, Indonesia and other Asian countries revealed by mtDNA Cyt b gene sequences and crossing experiments. (2013)
  • Author(s): M Hasan・MM Islam・WMM Nalley・M Matsui・M Sumida
  • Organizer: 日本爬虫両生類学会第52回大会
  • Place of Presentation: 札幌市
• [Presentation] 東アジア産トノサマガエル種群の系統関係と遺伝子浸透 (2013)
  • Author(s): 小巻翔平・井川武・倉林敦・Min-Sook Min・Si-Min Lin・住田正幸
  • Organizer: 日本動物学会第84回大会
  • Place of Presentation: 岡山市
• [Presentation] アフリカツメガエルのゲノム倍数化過程に生じた染色体再配列と無尾両生類における性染色体の起源とその進化に関する分子細胞遺伝学的研究 (2013)
  • Author(s): 宇野好宣・西田千鶴子・高木知世・井川武・上野直人・住田正幸・松田洋一
  • Organizer: 日本遺伝学会第52回大会
  • Place of Presentation: 横浜市
• [Presentation] Molecular cytogenetic studies on the process of genomic and chromosomal evolution in Xenopus laevis after whole-genome duplication and the origin and evolution of sex chromosomes in anuran species. (2013)
  • Author(s): Uno, Y., Nishida, C., Takagi, C., Igawa, T., Ueno, N., Sumida, M., and Matsuda, Y.
  • Organizer: 19th International Chromosome Conference
  • Place of Presentation: Bologna, Italia
• [Presentation] ウシガエルはどこから来て,なぜ駆逐されないのか? : 侵入成功と個体群存続における遺伝的要因の調査(ポスター発表) (2012)
  • Author(s): 徳増大輔,井川武, Min Mi-Sook,住田正幸
  • Organizer: 日本爬虫両生類学会第51回大会
  • Place of Presentation: 愛知
  • Year and Date: 2012-11-10
• [Presentation] マイクロサテライトマーカーを用いた絶滅危惧種および天然記念物イボイモリの遺伝的集団構造の解明(口頭発表) (2012)
  • Author(s): 井川武・菅原弘貴・本多正尚・富永篤・大海昌平・勝連盛輝・太田英利・松井正文・住田正幸
  • Organizer: 日本遺伝学会第84回大会
  • Place of Presentation: 福岡
  • Year and Date: 2012-09-25
• [Presentation] A case study of conservation genetics for endangered species in Japan : Population structures and its causal landscapes of two endangered frog species of genus Odorrana (2012)
  • Author(s): Igawa, T., Oumi, S., Katsuren, S., Sumida, M.
  • Organizer: Basic Science International Conference 2012
  • Place of Presentation: Malang, Indonesia
  • Year and Date: 2012-02-25
  • Invited
• [Presentation] 中央琉球産絶滅危惧両生類5種の遺伝的分化と飼育下繁殖 (2012)
  • Author(s): 住田正幸,井川武, Islam IMohammed Mafizul Islam, 新谷望, 掛橋竜祐, 菅原弘貴, 倉林敦, 大海昌平, 勝連盛輝, 田戸美雪, 藤井保
  • Organizer: 日本動物学会 第83回大会
  • Place of Presentation: 豊中市
• [Presentation] マイクロサテライトマーカーを用いた絶滅危惧種および天然記念物イボイモリの遺伝的集団構造の解明 (2012)
  • Author(s): 井川武, 菅原弘貴, 本多正尚, 富永篤, 大海昌平, 勝連盛輝, 太田英利, 松井正文, 住田正幸
  • Organizer: 日本遺伝学会 第84回大会
  • Place of Presentation: 福岡市
• [Presentation] アマミイシカワガエルの飼育下における温度条件と遺伝的要因が個体生存率に与える影響 (2012)
  • Author(s): 新谷望, 井川武, 住田正幸
  • Organizer: 日本爬虫両生類学会 第51回大会
  • Place of Presentation: 豊田市
• [Presentation] 南西諸島の絶滅危惧両生類における遺伝的分化と飼育下繁殖 (2012)
  • Author(s): 住田正幸, 井川武, IMohammed Mafizul Islam, 新谷望, 掛橋竜祐, 菅原弘貴, 西谷琢磨, 倉林敦, 大海昌平, 勝連盛輝, 田戸美雪, 藤井保
  • Organizer: 日本爬虫両生類学会 第51回大会
  • Place of Presentation: 豊田市
• [Presentation] A case study of conservation genetics for endangered species in Japan: Population structures and its causal landscapes of two endangered frog species of genus Odorrana (2012)
  • Author(s): Takeshi Igawa, Shohei Oumi, Seiki Katsuren, Masayuki Sumida
  • Organizer: 2nd Annual Basic Science International Conference (BaSIC) 2012(招待講演)
  • Place of Presentation: Malang, Indonesia
• [Presentation] 絶滅危惧種イシカワガエルにおける集団構造とその景観要因(口頭発表) (2011)
  • Author(s): 井川武,大海昌平,勝連盛輝,住田正幸
  • Organizer: 日本爬虫両棲類学会第50回記念大会
  • Place of Presentation: 京都
  • Year and Date: 2011-10-09
• [Presentation] Population structures and its causal landscapes of two endangered frog species of genus Odorrana -Different scenarios in two islands (Selected for oral presentation) (2011)
  • Author(s): Igawa, T., Oumi, S., Katsuren, S., and Sumida, M.
  • Organizer: Moleculaer Biology and Evolution (SMBE)
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2011-07-27
• [Presentation] Population structures and its causal landscapes of two endangered frog species of genus Odorrana - Different scenarios in two islands (2011)
  • Author(s): Takeshi Igawa, Shohei Oumi, Seiki Katsuren, Masayuki Sumida
  • Organizer: Society for Molecular Evolution 2011
  • Place of Presentation: 京都市
• [Presentation] 絶滅危惧種イシカワガエルにおける集団構造とその景観要因 (2011)
  • Author(s): 井川武, 大海昌平, 勝連盛輝, 住田正幸
  • Organizer: 日本爬虫両棲類学会 第50回記念大会
  • Place of Presentation: 京都市
• [Presentation] 絶滅危惧種イボイモリのマイクロサテライトDNAを用いた集団構造解析と飼育下繁殖の試み (2011)
  • Author(s): 菅原弘貴, 井川武, 大海昌平, 勝連盛輝, 横川昌史, 井鷺裕司, 住田正幸
  • Organizer: 日本爬虫両棲類学会 第50回記念大会
  • Place of Presentation: 京都市
• [Remarks]
  • URL: https://sites.google.com/site/takeshi2igawa/
• [Remarks] Takeshi Igawa Ph. D.
  • URL: https://sites.google.com/site/takeshi2igawa/
• [Remarks] 現在の研究テーマ - Web of Takeshi Igawa Ph.D.
  • URL: https://sites.google.com/site/takeshi2igawa/in-japanese/theme
• [Remarks] 業績 - Web of Takeshi Igawa Ph.D.
  • URL: https://sites.google.com/site/takeshi2igawa/in-japanese/publication