Experimental elucidation of dynamic behaviors to facilitate protein functions
| Project/Area Number |
23750015
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Physical chemistry
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| Research Institution | Osaka University |
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Principal Investigator |
MIZUNO Misao 大阪大学, 理学(系)研究科(研究院), 助教 (10464257)
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| Project Period (FY) |
2011-04-28 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 共鳴ラマン分光法 / 時間分解分光法 / 微生物型ロドプシン / タンパク質ダイナミクス / 光異性化 / イオン輸送 / 光センサー / 古細菌型ロドプシン |
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| Outline of Final Research Achievements |
Microbial rhodopsins function as either light-driven ion pumps or photosensors. In this study, structural dynamics of microbial rhodopsins were revealed by time-resolved resonance Raman spectroscopy. First, protein responses to photoisomerization of the retinal chromophore in the picosecond regime were investigated. Spectral changes in the ultraviolet resonance Raman bands due to the tryptophan and tyrosine residues in the vicinity of the chromophore were observed. It was found that the rates of primary protein response to the chromophore isomerization are similar among the rhodopsins. Second, temporal evolutions of the chromophore structure after nanoseconds were investigated by visible resonance Raman spectroscopy. For ion pumps, we revealed the structural changes of the hydrogen-bond network around the retinal chromophore which facilitate the ion pumping.
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Report
(5 results)
Research Products
(22 results)
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[Journal Article] Large spectral change due to amide modes of a β-sheet upon the formation of an early photointermediate of middle rhodopsin2013
Author(s)
Furutani, Y., Okitsu, T., Reissig, L., Mizuno, M., Homma, M., Wada, A., Mizutani, Y., Sudo, Y.
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Journal Title
J. Phys. Chem. B
Volume: 117
Issue: 13
Pages: 3449-3458
DOI
Related Report
Peer Reviewed
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