Development of the novel principles of electrochemical sensors using zymogens as sensing elements
| Project/Area Number |
23750073
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Analytical chemistry
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| Research Institution | Tohoku University |
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Principal Investigator |
INOUE Kumi 東北大学, マイクロシステム融合研究開発センター, 助教 (20597249)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | バイオセンサ / 電気化学 / 酵素前駆体 / チップ型電極 / 生体機能利用 |
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| Research Abstract |
We have developed novel type electrochemical sensors using zymogens as sensor elements. First, we made electrochemical endotoxin sensors based on the Limulus amebocyte lysate reagent containing zymogens. We successfully developed a cost effective screen-printed sensor chip with practical sensitivity. We also developed a highly sensitive device with a practical structure for stripping voltammetry. Then, we extended this zymogen-based sensing principle into other assays. We successfully detected the apoptosis of HepG2 cells using electrochemical methods. These results contribute not only the development of the sensor engineering but also the clinical safety and biological studies.
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Report
(4 results)
Research Products
(28 results)
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[Presentation] リムルス試薬を用いた電気化学検出型の小型エンドトキシン検査装置の開発2013
Author(s)
井上(安田)久美,高野真一朗,高橋里子,石田洋祐,川端荘平,水村光,小田俊男,小川健一,坪井達也,伊野浩介,珠玖仁,末永智一
Organizer
第19回日本エンドトキシン・自然免疫研究会
Place of Presentation
ロイヤルオークホテルスパ&ガーデンズ、大津
Year and Date
2013-12-07
Related Report
Invited
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