Development of sensitive aptasensors based on AP-site-binding ligands and Rolling cycle amplification.

• Project/Area Number: 23750074
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Analytical chemistry
• Research Institution: Tohoku University
• Principal Investigator: XU Zhiai 東北大学, 大学院・理学研究科, 助教 (50562336)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: アプタマー / モルキュラービーコン / 蛍光リガンド / L-アルギニアミド / アデ ノシン / DNA / RNA / 化学センサー / ラベルフリー検出 / 結合性リガンド / molecular beacons / RCA / L-argininamide

Research Abstract

In one part of this project, we have examined the binding of several different ligands such as flavones and naphthyridines to AP-site-containing DNA, RNA or DNA/RNA hybrid duplexes. The other part of this project is to develop new kinds of aptasensors based on these ligands and engineered AP-site-containing aptamers.

Research Products

• [Journal Article] Base-Pairing at the AbasicSite in DNA Duplexes and Its Application for Adenosine Aptasensors (2012)
  • Author(s): Y. Pang, Z. Xu, Y. Sato, S. Nishizawa and N. Teramae
  • Journal Title: ChemBioChem Volume: 13 Issue: 3 Pages: 436-442
  • DOI: 10.1002/cbic.201100666
  • Peer Reviewed
• [Journal Article] Fluorescence detection of small molecules based on adaptive transitions of abasic site-containing aptamers in combination with abasic site-binding ligands (2011)
  • Author(s): Z. Xu, Y. Sato, S. Nishizawa, and N. Teramae
  • Journal Title: Biosens. Bioelectron. Volume: 26 Issue: 12 Pages: 4733-4738
  • DOI: 10.1016/j.bios.2011.05.051
  • Peer Reviewed
• [Presentation] A signal-on ATP aptasensor based on nucleobase-specific fluorescent ligands (2011)
  • Author(s): Z. Xu, Y. Sato, S. Nishizawa, N. Teramae
  • Organizer: The 38th International Symposium on Nucleic Acid Chemistry (ISNAC 2011)
  • Place of Presentation: Sapporo, Japan
  • Year and Date: 2011-12-09
• [Presentation] Label-free aptasensors based on abasic site-containing DNA and nucleobase-specific fluorescent ligands (2011)
  • Author(s): 徐志愛
  • Organizer: 第28回無機分析コロキュウム
  • Place of Presentation: 東北大学 川渡共同セミナーセンター
  • Year and Date: 2011-06-10
• [Presentation] Adenosine sensor based on AP site-containing DNA duplex and fluorescent ligand (2011)
  • Author(s): Z. Xu, Y. Pang, Y. Sato, S. Nishizawa, N. Teramae
  • Organizer: IUPAC International Congress on Analytical Sciences 2011 (ICAS 2011)
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2011-05-25
• [Presentation] Label-free aptasensors based on abasic site-containing DNA and nucleobase-specific fluorescent ligands (2011)
  • Author(s): 徐 志愛
  • Organizer: 第２８回無機分析コロキュウム
  • Place of Presentation: 東北大学 川渡共同セミナーセンター
• [Remarks] (東北大学大学院理学研究科化学専攻分析化学研究室)
  • URL: http://www.anal.chem.tohoku.ac.jp/