Development of sensitive aptasensors based on AP-site-binding ligands and Rolling cycle amplification.
| Project/Area Number |
23750074
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Analytical chemistry
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| Research Institution | Tohoku University |
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Principal Investigator |
XU Zhiai 東北大学, 大学院・理学研究科, 助教 (50562336)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | アプタマー / モルキュラービーコン / 蛍光リガンド / L-アルギニアミド / アデ ノシン / DNA / RNA / 化学センサー / ラベルフリー検出 / 結合性リガンド / molecular beacons / RCA / L-argininamide |
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| Research Abstract |
In one part of this project, we have examined the binding of several different ligands such as flavones and naphthyridines to AP-site-containing DNA, RNA or DNA/RNA hybrid duplexes. The other part of this project is to develop new kinds of aptasensors based on these ligands and engineered AP-site-containing aptamers.
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Report
(3 results)
Research Products
(7 results)
