Development of slab optical waveguide fluorescent microscopy system for the study of heterogeneous chemical reactions of biochemical agents at lipid membrane

Research Project

Project/Area Number	23750084
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Analytical chemistry
Research Institution	Nara University of Education
Principal Investigator	HOTTA Hiroki 奈良教育大学, 教育学部, 准教授 (80397603)
Project Period (FY)	2011 – 2012
Project Status	Completed (Fiscal Year 2012)
Budget Amount *help	¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000) Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000) Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywords	化学センサー / 界面反応計測 / 平面リン脂質膜 / DMPC / コレステロール / 抗菌剤 / グラミシジンS改変体
Research Abstract	Slab optical waveguide fluorescent microscopy (SOWG-FM) system,which was developed by research representatives was applied to the observation of lipid membrane disruption by antibacterial agent, Gramicidin S (GS). The measurement system has an important advantage in the observation of planar lipid membrane stabilized on a glass surface because its high interfacial selectivity using an evanescent wave illumination.The research representative was developed a visualization method of the planar lipid membrane by an environmentally-responsive fluorescent dye. Lipid membrane disruption by GS and its analog were observed. The results showed good correlation with those antibacterial activities.