Development of enzyme free sensitive gene detection system based ontemplate-directed formation of luminescent lanthanide complexe
| Project/Area Number |
23750090
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Analytical chemistry
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| Research Institution | Kumamoto University |
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Principal Investigator |
KITAMURA Yusuke 熊本大学, 大学院・自然科学研究科, 助教 (80433019)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
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| Keywords | 生体分析 / 核酸プローブ / 遺伝子解析 / 希土類金属錯体 / SNPs / 核酸 / バイオ分析 / プローブ |
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| Research Abstract |
We present the facile technique of colorimetric SNP (single nucleotide polymorphism)analysis through DNA-templated cooperative complexation between a luminescent lanthanideion (Ln(III): Tb(III) or Eu(III)) and two ODN (oligodeoxyribonucleotide) conjugatescarrying a metal chelator. EDTA (ethylenediaminetetraacetic acid)) and1,10-phenanthroline (phen) were covalently attached to ODNs to form conjugates forapplication as capture and sensitizer probes. The sequences of the conjugates weredesigned so as to form a ternary tandem duplex with the target, where their auxiliaryunits face each other, providing a microenvironment to accommodate Ln(III). Thecharacteristic emissions of the Tb(III) and Eu(III) ions were clearly observed in thepresence of the full-matched target DNA. On the other hand, the emissions from the mixedsolutions with the mutant containing one base displacement were marginal.
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Report
(3 results)
Research Products
(27 results)
