| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
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| Research Abstract |
We present the facile technique of colorimetric SNP (single nucleotide polymorphism)analysis through DNA-templated cooperative complexation between a luminescent lanthanideion (Ln(III): Tb(III) or Eu(III)) and two ODN (oligodeoxyribonucleotide) conjugatescarrying a metal chelator. EDTA (ethylenediaminetetraacetic acid)) and1,10-phenanthroline (phen) were covalently attached to ODNs to form conjugates forapplication as capture and sensitizer probes. The sequences of the conjugates weredesigned so as to form a ternary tandem duplex with the target, where their auxiliaryunits face each other, providing a microenvironment to accommodate Ln(III). Thecharacteristic emissions of the Tb(III) and Eu(III) ions were clearly observed in thepresence of the full-matched target DNA. On the other hand, the emissions from the mixedsolutions with the mutant containing one base displacement were marginal.
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