Analysis of cellular functions in primary cells usingscanning electrochemical microscopy

• Project/Area Number: 23750095
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Analytical chemistry
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: HIRANO Yu 独立行政法人産業技術総合研究所, 生物プロセス研究部門, 主任研究員 (70415735)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 生体分析 / 電気化学 / SECM / 細胞 / マイクロ電極

Research Abstract

Analysis of primary-cell culture is currently used to evaluate the pharmacological and toxicological properties of drugs. We developed a SECM system for analyzing contraction kinetics in cardiomyocytes and cellular volume in adipocyte. This system enabled

Research Products

• [Journal Article] Development of a scanning electrochemical microscopy-based micropipette and its application to analysis of topographic change of single-cell (2013)
  • Author(s): 平野 悠
  • Journal Title: bioelectrochemistry Volume: 92 Pages: 1-5
  • DOI: 10.1016/j.bioelechem.2013.01.004
  • Peer Reviewed
• [Journal Article] Improvement of Detectable Sensitivity for Enzyme Reaction by Scanning Electrochemical Microscopy with Distance Control System for Immunosensing (2012)
  • Author(s): Yu Hirano, Tomoyuki Yasukawa, Yoshiaki Mase, Daisuke Oyamatsu, Hitoshi Shiku, Fumio Mizutani, Tomokazu Matsue
  • Journal Title: Electrochemistry Volume: 80 Issue: 1 Pages: 30-32
  • DOI: 10.5796/electrochemistry.80.30
  • NAID: 10029981363
  • ISSN: 1344-3542, 2186-2451
  • Peer Reviewed
• [Journal Article] Interstrand cross-link of DNA by covalently linking a pair of abasic sites (2012)
  • Author(s): Ichikawa, K., Kojima, N., Hirano, Y., Takebayashi, T., Kowata, K. and Komatsu, Y
  • Journal Title: Chem. Commun.(Camb) Volume: 48 Issue: 15 Pages: 2143-2145
  • DOI: 10.1039/c2cc16785a
  • Peer Reviewed
• [Presentation] Electrochemical monitoring of enzyme reactions promoted on double stranded DNA (2011)
  • Author(s): Y. Hirano, K. Ichikawa, N. Kojima, and Y. Komatsu
  • Organizer: 第38回国際核酸化学シンポジウム
  • Place of Presentation: 北海道大学(北海道)
  • Year and Date: 2011-11-10
• [Presentation] Electrochemical monitoring of enzyme reactions promoted on double stranded DNA (2011)
  • Author(s): 平野 悠
  • Organizer: 第38回国際核酸化学シンポジウム
  • Place of Presentation: 札幌 北海道大学