• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Hydrated ionic liquid for dissolution and refolding of proteins

Research Project

Project/Area Number 23750120
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Polymer chemistry
Research InstitutionTokyo University of Agriculture and Technology

Principal Investigator

FUJITA Kyoko  東京農工大学, 工学(系)研究科(研究院), 講師 (90447508)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsイオン液体 / 生体高分子 / 水和状態 / タンパク質 / リフォールディング
Research Abstract

Physicochemical property of hydrated ionic liquid which is composed by cholinium cation ([ch]) and phosphate anion ([dhp]) has been analyzed with changing the water contents. The water molecules were interacted with ions strongly and existed as bound water. Proteins and nucleobase pairs were dissolved in hydrated [ch][dhp] with remained the higher ordered structure. Furthermore, the activity of dissolved biomolecules in hydrated [ch][dhp] were observed by spectroscopically and electrochemically. Furthermore, thermal stability and long term stability of the responses has been improved in hydrated [ch][dhp].

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (24 results)

All 2014 2013 2012 2011 Other

All Journal Article (8 results) (of which Peer Reviewed: 7 results) Presentation (14 results) Remarks (2 results)

  • [Journal Article] 2-(Trimethylazaniumyl)ethyl hydrogen phosphate (phosphocholine) monohydrate2014

    • Author(s)
      Y. Nikawa, K. Fujita, K. Noguchi, H. Ohno
    • Journal Title

      Acta Cryst .E

      Volume: E70 Issue: 5 Pages: o549-o549

    • DOI

      10.1107/s160053681400779x

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Cold crystallisation behaviour of water molecules in ionic liquids as a screening method to evaluate biocompatibility of the hydrated ionic liquids2013

    • Author(s)
      K. Fujita, Y. Nikawa, and H. Ohno
    • Journal Title

      Chem. Comm

      Volume: 49 Issue: 31 Pages: 3257-3259

    • DOI

      10.1039/c3cc39033k

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Direct dissolution of wet and saliferous marine microalgae using polar ionic liquids without heating2013

    • Author(s)
      K. Fujita, D. Kobayashi, N. Nakamura, and H. Ohno
    • Journal Title

      Enzyme and Microbial Technology

      Volume: 52 Issue: 3 Pages: 199-202

    • DOI

      10.1016/j.enzmictec.2012.12.004

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Contracting cardiomyocytes in hydrophobic room-temperature ionic liquid2012

    • Author(s)
      T. Hoshino, K. Fujita, A. Higashi, K. Sakiyama, H. Ohno, and K. Morishima
    • Journal Title

      Biochem. Biophys. Res. Commun

      Volume: 427 Issue: 2 Pages: 379-384

    • DOI

      10.1016/j.bbrc.2012.09.068

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Stable G-Quadruplex Structure in a Hydrated Ion Pair: Cholinium Cation and Dihydrogen Phosphate Anion2012

    • Author(s)
      K. Fujita
    • Journal Title

      Chem. Commun

      Volume: 48 Issue: 46 Pages: 5751-5753

    • DOI

      10.1039/c2cc30554b

    • Related Report
      2013 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Ionic liquids designed for advanced applications in bioelectrochemistry2012

    • Author(s)
      K. Fujita
    • Journal Title

      RSC Advances

      Volume: 2 Issue: 10 Pages: 4018-4030

    • DOI

      10.1039/c2ra01045c

    • Related Report
      2013 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] タンパク質を未変性で溶解できるイオン液体の設計2011

    • Author(s)
      藤田恭子、田村薫、大野弘幸
    • Journal Title

      ファインケミカル

      Volume: 40 Pages: 31-36

    • Related Report
      2013 Final Research Report 2011 Research-status Report
  • [Journal Article] Preparation of an Electrode Modified with an Electropolymerized Film as a Mediator of NADH Oxidation and Its Application in an Ethanol/O2 Biofuel Cell2011

    • Author(s)
      M. Masuda
    • Journal Title

      Electroanalysis

      Volume: 23 Issue: 10 Pages: 2297-2301

    • DOI

      10.1002/elan.201100215

    • Related Report
      2013 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Presentation] イオン液体を用いた不溶化セルラーゼの溶解とリフォールディング2014

    • Author(s)
      梶山万悠子、藤田恭子、劉遠、中村暢文、大野弘幸
    • Organizer
      日本化学会第94春季年会
    • Place of Presentation
      名古屋大学
    • Year and Date
      2014-03-30
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] コリニウムリン酸二水素を基本構造とする水和イオン液体の解析とタンパク質溶媒としての評価2013

    • Author(s)
      藤田恭子、大野弘幸
    • Organizer
      第62回高分子討論会
    • Place of Presentation
      金沢大学
    • Year and Date
      2013-09-12
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] Hydration state of choline dihydrogen phosphate and analogous ionic liquids2013

    • Author(s)
      K. Fujita, H. Ohno
    • Organizer
      Post-symposium on Ionic Liquids from Science to Green Chemical Applications
    • Place of Presentation
      Tokyo, Japan
    • Year and Date
      2013-07-13
    • Related Report
      2013 Final Research Report
  • [Presentation] Cold crystallization behavior of co-exissted water molecules observed in dihydrogen phosphate analogs2013

    • Author(s)
      K. Fujita, Y. Nikawa, H. Ohno
    • Organizer
      5^<th> International Congress on Ionic Liquids
    • Place of Presentation
      Vilamoura, Portugal
    • Year and Date
      2013-04-22
    • Related Report
      2013 Final Research Report
  • [Presentation] G-quadruplex formation in hydrated ionic liquids2013

    • Author(s)
      K. Fujita, H. Ohno
    • Organizer
      5^<th> International Congress on Ionic Liquids
    • Place of Presentation
      Vilamoura, Portugal
    • Year and Date
      2013-04-22
    • Related Report
      2013 Final Research Report
  • [Presentation] 誘電分光法による水和イオン液体の分子ダイナミクス評価2012

    • Author(s)
      藤田恭子、澤田拓也、喜多理王、新屋敷直木、八木原晋、大野弘幸
    • Organizer
      イオン液体研究会
    • Place of Presentation
      沖縄県男女共同参画センター「てぃいる」
    • Year and Date
      2012-12-07
    • Related Report
      2013 Final Research Report
  • [Presentation] 誘電分光法による水和イオン液体の分子ダイナミクス評価2012

    • Author(s)
      藤田 恭子、澤田 拓也、喜多 理王、新屋敷 直木、八木原 晋、大野 弘幸
    • Organizer
      イオン液体研究会
    • Place of Presentation
      沖縄県男女共同参画センター 「てぃるる」
    • Related Report
      2012 Research-status Report
  • [Presentation] 水和イオン液体中でのDNA 四重鎖構造形成の検討2011

    • Author(s)
      藤田恭子、大野弘幸
    • Organizer
      第2回イオン液体討論会
    • Place of Presentation
      キャンパスプラザ京都
    • Year and Date
      2011-12-17
    • Related Report
      2013 Final Research Report
  • [Presentation] Effectiveness of hydrated ionic liquid composed of choline cation and dihydrogen phosphate anion as a medium for biomolecules2011

    • Author(s)
      K. Fujita, H. Ohno
    • Organizer
      The 4^<th> International Congress on Ionic Liquids (COIL-4)
    • Place of Presentation
      Hilton crystal city, Washington DC
    • Year and Date
      2011-06-17
    • Related Report
      2013 Final Research Report
  • [Presentation] Effectiveness of hydrated ionic liquid composed of choline cation and dihydrogen phosphate anion as a medium for biomolecules2011

    • Author(s)
      Kyoko Fujita and Hiroyuki Ohno
    • Organizer
      The 4th International Congress on Ionic Liquids (COIL-4)
    • Place of Presentation
      Hilton crystal city, Washington DC
    • Related Report
      2011 Research-status Report
  • [Presentation] 水和イオン液体中でのDNA四重鎖構造形成の検討2011

    • Author(s)
      藤田恭子、大野弘幸
    • Organizer
      第2回イオン液体討論会
    • Place of Presentation
      キャンパスプラザ京都
    • Related Report
      2011 Research-status Report
  • [Presentation] Cold crystallization behavior of co-exissted water molecules observed in dihydrogen phosphate analogs

    • Author(s)
      K. Fujita, Y. Nikawa, H. Ohno
    • Organizer
      5th International Congress on Ionic Liquids
    • Place of Presentation
      Vilamoura, Portugal
    • Related Report
      2013 Annual Research Report
  • [Presentation] G-quadruplex formation in hydrated ionic liquids, 5th International Congress on Ionic Liquids, Vilamoura

    • Author(s)
      K. Fujita, H. Ohno
    • Organizer
      5th International Congress on Ionic Liquids
    • Place of Presentation
      Vilamoura, Portugal
    • Related Report
      2013 Annual Research Report
  • [Presentation] Hydration state of choline dihydrogen phosphate and analogous ionic liquids

    • Author(s)
      K. Fujita, H. Ohno
    • Organizer
      Post-symposium on Ionic Liquids from Science to Green Chemical Applications
    • Place of Presentation
      AIST Tokyo Waterfront, Tokyo
    • Related Report
      2013 Annual Research Report
  • [Remarks] ホームページ等 (大野研ホームページ内)

    • URL

      http://www.tuat.ac.jp/~ohno/index.html

    • Related Report
      2013 Final Research Report
  • [Remarks] たんぱく質・生体分子の新規溶媒としての水和イオン液体の提案

    • URL

      http://www.tuat.ac.jp/~ohno/research/ohno-biotech.html#res2

    • Related Report
      2013 Annual Research Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi