Development of hyper signal amplification method by fluorogenic reaction of oligonucleotides
| Project/Area Number |
23750178
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Chemistry related to living body
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| Research Institution | Hokkaido University |
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Principal Investigator |
SATO Kousuke 北海道大学, 大学院・薬学研究院, 助教 (70415686)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 核酸 / 蛋白質 / 糖化学 / 生物有機化学 / 核酸関連化学 |
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| Research Abstract |
We synthesized a nucleoside phosphoramidite containing aaminothiophenol structure for cross-link with 5-formyl-2 ' -deoxyuridine.Oligodeoxyribonucleotide (ODN) were also synthesized by DNA synthesizer, and deprotectedand purified. The reaction between ODN containing aminothiophenol and 5-formy-2 ' -deoxyuridine was investigated on the complementary template ODN. We also designed thesequence of ODNs by computational modeling.
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Report
(3 results)
Research Products
(32 results)
