Activation of target genes with crosslink-forming oligonucleotide

• Project/Area Number: 23750180
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Chemistry related to living body
• Research Institution: Tohoku University
• Principal Investigator: HAGIHARA Shinya 東北大学, 多元物質科学研究所, 助教 (80373348)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
• Keywords: 核酸関連化学 / miRNA / 架橋性核酸

Research Abstract

Crosslink forming oligonucleotide (CFO) was used for target gene specific Inhibition of microRNA (miRNA) functions. The method can interfere with specific miRNA-mRNA interactions by recognizing the sequences unique to the 3'-UTR that are inherent in each mRNAs.

Research Products

• [Journal Article] Production of truncated protein by the crosslink formation of mRNA with 2’-OMe oligoribonucleotide containing 2-amino-6-vinylpurine (2012)
  • Author(s): Hagihara, S., Kusano, S., Lin, W.C., Chao, X.G., Hori, T., Imoto, S., Nagatsugi, F.
  • Journal Title: Bioorg. Med. Chem. Lett. Volume: 22 Issue: 12 Pages: 3870-3872
  • DOI: 10.1016/j.bmcl.2012.04.123
  • Peer Reviewed
• [Journal Article] ynthesis of 6-amino-2-vinyl purine derivatives for cross-linking and evaluation of the reactivity (2012)
  • Author(s): Kusano,S., Sakuraba, T., Hagihara, S., Nagatsugi, F.
  • Journal Title: Bioorg. Med. Chem. Lett. Volume: 22 Issue: 22 Pages: 6957-6951
  • DOI: 10.1016/j.bmcl.2012.08.122
  • Peer Reviewed
• [Presentation] 架橋性オリゴ核酸を用いた細胞内遺伝子発現制御 (2013)
  • Author(s): 萩原伸也、草野修平、井田裕太、岩本直生、永次史
  • Organizer: 日本化学会第93春季年会
  • Place of Presentation: 立命館大学
  • Year and Date: 2013-03-25
• [Presentation] 立体配座を固定化した新規架橋性分 子の開発 (2013)
  • Author(s): 井田裕太、草野修平、岩本直生、萩原伸也、永次史
  • Organizer: 日本化学会第93春季年会
  • Place of Presentation: 立命館大学
  • Year and Date: 2013-03-25
• [Presentation] 架橋性オリゴ核酸を用いた細胞内遺伝子発現制御 (2013)
  • Author(s): 萩原伸也
  • Organizer: 日本化学会第９３春季年会
  • Place of Presentation: 立命館大学
• [Presentation] 立体配座を固定化した新規架橋性分子の開発 (2013)
  • Author(s): 井田裕太
  • Organizer: 日本化学会第９３春季年会
  • Place of Presentation: 立命館大学
• [Presentation] 機能性オリゴ核酸のRNAへの架橋形成による遺伝子発現制御 (2012)
  • Author(s): 萩原伸也、草野修平、鈔暁光、岩本直生、永次史
  • Organizer: 第92日本化学会春季年会
  • Place of Presentation: 神奈川
  • Year and Date: 2012-03-26
• [Presentation] 2'-OMe RNA containing 2-amino-6-vinylpurine as a potent gene regulator (2011)
  • Author(s): 萩原伸也、井本修平、鈔暁光、堀恒晃、草野修平、永次史
  • Organizer: 第38際核酸化学シンポウム
  • Place of Presentation: 札幌
  • Year and Date: 2011-11-09
• [Presentation] 2'-OMe RNA containing 2-amino-6-vinylpurine as a potent gene regulator (2011)
  • Author(s): 萩原伸也、井本修平、鈔暁光、堀恒晃、草野修平、永次史
  • Organizer: 第38回国際核酸化学シンポジウム
  • Place of Presentation: 札幌