Construction of PCR-like amplification system of artificial metallo-DNAs
| Project/Area Number |
23750181
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Chemistry related to living body
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| Research Institution | The University of Tokyo |
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Principal Investigator |
TAKEZAWA Yusuke 東京大学, 理学(系)研究科(研究院), 助教 (70508598)
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| Project Period (FY) |
2011-04-28 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 人工DNA / 金属錯体型塩基対 / 超分子化学 / 錯体化学 / 酵素合成 / 自己複製 |
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| Outline of Final Research Achievements |
This research aims to construct self-replicating artificial metallo-DNA duplexes, in which natural hydrogen-bonded base pairs are replaced by artificial metal-mediated base pairs. In this study, we have developed a novel metal-mediated base pair and investigated various condensation reactions of artificial ligand-bearing nucleotides. It was found that artificial oligonucleotides possessing multiple hydroxypyridone-bearing nucleotides, which form Cu(II)-mediated base pairs, were successfully synthesized by using a template-independent DNA polymerase. Furthermore, a novel metallo-DNA duplex was constructed using a 5-hydroxyuracil nucleobase as a metal ligand, which formed Gd(III)-mediated base pairs.
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Report
(5 results)
Research Products
(28 results)
