| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
This research aims to construct self-replicating artificial metallo-DNA duplexes, in which natural hydrogen-bonded base pairs are replaced by artificial metal-mediated base pairs. In this study, we have developed a novel metal-mediated base pair and investigated various condensation reactions of artificial ligand-bearing nucleotides. It was found that artificial oligonucleotides possessing multiple hydroxypyridone-bearing nucleotides, which form Cu(II)-mediated base pairs, were successfully synthesized by using a template-independent DNA polymerase. Furthermore, a novel metallo-DNA duplex was constructed using a 5-hydroxyuracil nucleobase as a metal ligand, which formed Gd(III)-mediated base pairs.
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