| Project/Area Number |
23750182
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Chemistry related to living body
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | タンパク質 / 細胞・組織 / 生体分子 / 1 分子計測(SMI) / 生体関連化学 / 機能性分子 / 光制御 / 1分子イメージング / シグナル伝達 / 蛋白質 / 1分子計測（SMD） |
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| Research Abstract |
The purpose of this study is to understand importance of receptor cluster formation in physiological signal transduction. For the purpose, I performed (1) single-molecule imaging of signal transduction molecule Akt, and (2) development of photo-controllingsystem of receptor nano-clusters. As the result of (1), I found out that Akt residency time on plasma membrane is elongated upon external signal input. This result indicates dynamics of Akt is affected by formation of receptor clusters. In the work (2), I succeeded in constructing a fusion protein of light-responsive protein and receptor, in addition to purified light responsive protein; meaning that I completed to prepare tools to produce photo-control system of receptor cluster formation. In conclusion, I showed the importance of receptor cluster to regulate signal transduction, and prepared a basis to prove the importance by a constitutive strategy.
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