| Project/Area Number |
23750187
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Chemistry related to living body
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| Research Institution | Nagoya Institute of Technology |
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Principal Investigator |
MIZUNO TOSHIHSIA 名古屋工業大学, 工学(系)研究科(研究院), 助教 (90345950)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | 導電性高分子 / 蛋白質 / ナノ粒子 / 蛍光プローブ / PEDOT / 共役性高分子 / 酸化還元応答プローブ |
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| Research Abstract |
Preparations of poly (3,4-ethylenedioxythiophene)/protein (PEDOT/protein) nanoparticle, consisting of the oligomeric PEDOTs and natural proteins were achieved by an oxidative polymerization of 3,4-ethylenedioxythiophene (EDOT) in the presence of partially denatured proteins in an aqueous solution containing 3 M Gdm HCl. From the dynamic light scattering (DLS) and the transmission electron microscope (TEM) observations, the hydrodynamic diameters of obtained nanoparticles were homogeneous by ~ 50 nm. We evaluated the redox potentials of PEDOT/protein nanoparticles using the cyclic voltammetric analyses and the observed redox potentials have a dependence on the choice of natural proteins. The current method to prepare the PEDOT/protein nanoparticle should be applicable to construct various kinds of the PEDOT/biomolecule nanoparticles, leading to the construction of redox-active sensor conjugates enable to use in vitro and in vivo.
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