Preparation and Application of polythiophene/protein conjugate

• Project/Area Number: 23750187
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Chemistry related to living body
• Research Institution: Nagoya Institute of Technology
• Principal Investigator: MIZUNO TOSHIHSIA 名古屋工業大学, 工学(系)研究科(研究院), 助教 (90345950)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 導電性高分子 / 蛋白質 / ナノ粒子 / 蛍光プローブ / PEDOT / 共役性高分子 / 酸化還元応答プローブ

Research Abstract

Preparations of poly (3,4-ethylenedioxythiophene)/protein (PEDOT/protein) nanoparticle, consisting of the oligomeric PEDOTs and natural proteins were achieved by an oxidative polymerization of 3,4-ethylenedioxythiophene (EDOT) in the presence of partially denatured proteins in an aqueous solution containing 3 M Gdm HCl. From the dynamic light scattering (DLS) and the transmission electron microscope (TEM) observations, the hydrodynamic diameters of obtained nanoparticles were homogeneous by ~ 50 nm. We evaluated the redox potentials of PEDOT/protein nanoparticles using the cyclic voltammetric analyses and the observed redox potentials have a dependence on the choice of natural proteins. The current method to prepare the PEDOT/protein nanoparticle should be applicable to construct various kinds of the PEDOT/biomolecule nanoparticles, leading to the construction of redox-active sensor conjugates enable to use in vitro and in vivo.

Research Products

• [Journal Article] Molecular Assembly of Chlorophyll Derivatives Using Recombinant Light-Harvesting Polypeptides with His-tag and Its Immobilization on a Gold Electrode (2013)
  • Author(s): S. Sakai, T. Noji, M. Kondo, T. Mizuno, T. Dewa, T. Ochiai, H. Yamakawa, S. Itoh, H. Hashimoto, M. Nango
  • Journal Title: Langmuir Volume: 29 Pages: 5104-5109
• [Journal Article] Applications of Peptide Gemini Surfactants as Solubilization Surfactants for Photosystems I and II of Cyanobacteria (2013)
  • Author(s): S. Koeda, K. Umezaki, T. Noji, A. Ikeda, K. Kawakami, M. Kondo, Y. Yamamoto, J. Shen, K. Taga, T. Dewa, M. Nango, T. Tanaka, T. Mizuno
  • Journal Title: Langmuir Volume: 29(37)
• [Journal Article] Creation of fibrous nanotube of green fluorescent protein by conjugation with pH-responsive polymer, poly (2-vinylpyridine), and use of a microfluidic synthesis (2013)
  • Author(s): N. Okiyama, E. Ota, A. Sumino, T. Noji, K. Yamamoto, J. Oku, T. Dewa, T. Tanaka, T. Mizuno
  • Journal Title: Chem. Lett. Volume: 42(5) Pages: 495-497
  • NAID: 10031167802
• [Journal Article] Creation of cross-linked bilayer membranes that can incorporate membrane proteins from oligo-Asp-based PG-surfactants (2013)
  • Author(s): S. Koeda, K. Umezaki, A. Sumino, A. Ikeda, Y. Yamamoto, T. Dewa, K. Taga, M. Nango, T. Tanaka, T. Mizuno
  • Journal Title: Langmuir Volume: 29(34)
• [Journal Article] Creation of cross-linked bilayer membranes that can incorporate membrane proteins from oligo-asp-based Peptide gemini surfactants. (2013)
  • Author(s): Koeda, S. ; Umezaki, K. ; Sumino, A. ; Noji, T. ; Ikeda, A. ; Yamamoto, Y. ; Dewa, T. ; Taga, K. ; Nango, M. ; Tanaka, T. ; Mizuno, T.
  • Journal Title: Langmuir Volume: 29 Issue: 37 Pages: 11695-11704
  • DOI: 10.1021/la401566h
  • Peer Reviewed
• [Journal Article] Applications of Peptide Gemini Surfactants as Solubilization Surfactants for Photosystems I and II of Cyanobacteria (2013)
  • Author(s): Shuhei Koeda, Katsunari Umezaki, Tomoyasu Noji, Atsushi Ikeda, Keisuke Kawakami, Masaharu Kondo, Yasushi Yamamoto, Jian-Ren Shen, Keijiro Taga, Takehisa Dewa, Mamoru Nango, Toshiki Tanaka, Toshihisa Mizuno
  • Journal Title: Langmuir Volume: 29 Issue: 37 Pages: 11667-11680
  • DOI: 10.1021/la402167v
  • Peer Reviewed
• [Journal Article] Creation of fibrous nanotube of green fluorescent protein by conjugation with pH-responsive polymer, poly (2-vinylpyridine), and use of a microfluidic synthesis (2013)
  • Author(s): Naoya Okiyama, Eriko Ota, Ayumi Sumino, Tomoyasu Noji, Katsuhiro Yamamoto, Jun-ichi Oku, Takehisa Dewa, Toshiki Tanaka, Toshihisa Mizuno
  • Journal Title: Chemistry Letters Volume: 42 Pages: 495-497
  • NAID: 10031167802
  • Peer Reviewed
• [Journal Article] Overexpression of Rhodobacter sphaeroides PufX bearing maltose-binding protein and its effect on the stability of reconstituted light-harvesting core antenna complex (2012)
  • Author(s): S. Sakai, A. Hiro, M. Kondo, T. Mizuno, T. Tanaka, T. Dewa, M. Nango
  • Journal Title: Photosynthesis Research Volume: 111(1-2) Pages: 63-69
• [Journal Article] Overexpression of Rhodobacter sphaeroides PufX bearing maltose-binding protein and its effect on the stability of reconstituted light-harvesting core antenna complex (2012)
  • Author(s): Shunnsuke Sakai, Akito Hiro, Masaharu Kondo, Toshihisa Mizuno, Toshiki Tanaka, Takehisa Dewa, Mamoru Nango
  • Journal Title: Photosynthesis Research Volume: 111 Pages: 63-69
  • Peer Reviewed
• [Journal Article] Formation of Planar Bilayer Membranes on Solid Supports Using Peptide Gemini Surfactants (2012)
  • Author(s): Katsunari Umezaki, Shunsuke Sakai, Shuhei Koeda, Yasushi Yamamoto, Masaharu Kondo, Atsushi Ikeda, Takehisa Dewa, Keijiro Taga, Toshiki Tanaka, Toshihisa Mizuno
  • Journal Title: Chemistry Letters Volume: 41
  • NAID: 10031126553
  • Peer Reviewed
• [Journal Article] Atomic force microscopy of the molecular assembly of the photosynthetic core antenna light harvesting (LH)1-type complex composed of the recombinant LH1-αpeptide bearing the maltose-binding protein (2011)
  • Author(s): S. Sakai, A. Sumino, A. Hiro, T. Mizuno, T. Dewa, T. Tanaka, H. Hashimoto, M. Nango
  • Journal Title: Chem. Lett. Volume: 40 Pages: 1280-1282
• [Journal Article] 異なる機能を持った蛋白質ドメインの組み合わせによる刺激応答性蛋白質の合理的設計 (2011)
  • Author(s): 水野稔久
  • Journal Title: 化学と教育 Volume: 59 Pages: 212-213
• [Presentation] PEDOT/Proteinナノ粒子の調製法の最適化 (2013)
  • Author(s): 市来健太郎、水野稔久、近藤政晴、出羽毅久、田中俊樹
  • Organizer: 第7回バイオ関連化学シンポジウム
  • Place of Presentation: 名古屋
  • Year and Date: 2013-09-27
• [Presentation] Facile Preparation of Water-Soluble Poly(3,4-ethylenedioxythiophene) (PEDOT)/Protein Nanoparticle (2013)
  • Author(s): Kentaro Ichiki, Takuya Ukon, Masaharu Kondo, Takehisa Dewa, Toshiki Tanaka, Toshihisa Mizuno
  • Organizer: IGER International Symposium on Cell Surface Structures and Functions
  • Place of Presentation: Naogya
  • Year and Date: 2013-09-02
• [Presentation] Creation of Bilayer Nanotube of Green Fluorescent Protein by Conjugating with pH-responsive Polymer, Poly (2-vinulpyridine) and Use of Microfluidic Synthesis (2013)
  • Author(s): Naoya Okiyama, Tomoyau Noji, Jun-ichi Oku, Takehisa Dewa, Toshiki Tanaka, Toshihisa Mizuno
  • Organizer: IGER International Symposium on Cell Surface Structures and Functions
  • Place of Presentation: Naogya
  • Year and Date: 2013-09-02
• [Presentation] PEDOT/Proteinナノ粒子の調製と機能評価 (2013)
  • Author(s): 市来健太郎、水野稔久、近藤政晴、出羽毅久、田中俊樹
  • Organizer: 第23回バイオ高分子シンポジウム
  • Place of Presentation: 神奈川
  • Year and Date: 2013-07-31
• [Presentation] タンパク質とPEDOTの複合化によるPEDOT/Proteinナノ粒子の調製 (2013)
  • Author(s): 市来健太郎、水野稔久、近藤政晴、出羽毅久、田中俊樹
  • Organizer: 第62回高分子学会年次大会
  • Year and Date: 2013-05-30
• [Presentation] PEDOT/Proteinナノ粒子の調製と生成機構の検討 (2013)
  • Author(s): 市来健太郎、水野稔久、近藤政晴、出羽毅久、田中俊樹
  • Organizer: 日本化学会第94春期年会
  • Place of Presentation: 名古屋
  • Year and Date: 2013-03-29
• [Presentation] 機能性ペプチドをグラフト化したGFPナノファイバーの調製と機能評価 (2013)
  • Author(s): 沖山直矢、水野稔久、大田英里子、角野歩、野地智康、出羽毅久、奥淳一、田中俊樹、杉安和憲、竹内正之
  • Organizer: 日本化学会第93春期年会
  • Place of Presentation: 滋賀
  • Year and Date: 2013-03-24
• [Presentation] 種々の蛋白質とPEDOTの複合化によるPEDOT/Proteinナノ粒子の調製 (2013)
  • Author(s): 市来健太郎、水野稔久、近藤政晴、出羽毅久、田中俊樹
  • Organizer: 日本化学会第93春期年会
  • Place of Presentation: 滋賀
  • Year and Date: 2013-03-22
• [Presentation] Creation of GFP Nanofiber by the Aid of pH-responsible Polymer Assembly (2012)
  • Author(s): N. Okiyama, T. Mizuno, E. Ohta, J. Oku, T. Tanaka, T. Dewa, K. Sugiyasu, M. Takeuchi
  • Organizer: The 9th SPSJ International Polymer Conference (IPC2012)
  • Place of Presentation: 神戸
  • Year and Date: 2012-12-14
• [Presentation] 緑色蛍光蛋白質-ポリ(2-ビニルピリジン)複合体の会合挙動評価 (2012)
  • Author(s): 沖山直矢、水野稔久、大田英理子、奥淳一、出羽毅久、田中俊樹、杉安和憲、竹内正之
  • Organizer: 第43回中部化学関係学協会支部連合秋季大会(名古屋)
  • Place of Presentation: 名古屋
  • Year and Date: 2012-11-10
• [Presentation] pH応答性高分子の付加によるGFPナノファイバーの作製 (2012)
  • Author(s): 沖山直矢、水野稔久、大田英理子、奥淳一、出羽毅久、田中俊樹、杉安和憲、竹内正之
  • Organizer: 第61回高分子討論会
  • Place of Presentation: (名工大) 名古屋
  • Year and Date: 2012-09-21
• [Presentation] Preparation and characterization of green fluorescent protein (GFP)-poly (2-vinylpyridine) conjugates (2012)
  • Author(s): N. Okiyama, E. Ohta, J. Oku, T. Dewa, T. Mizuno, T. Tanaka
  • Organizer: The 2nd Asian Chemical biology Conference
  • Place of Presentation: Itoman (Okinawa)
  • Year and Date: 2012-07-05
• [Presentation] 両親媒性蛋白質を利用したポリチオフェンナノ粒子の作製 (2012)
  • Author(s): 右近卓也、水野稔久、近藤政晴、出羽毅久、田中俊樹、杉安和憲、竹内正之
  • Organizer: 日本化学会第92春期年会(2012)
  • Place of Presentation: 横浜
  • Year and Date: 2012-03-27
• [Presentation] 緑色蛍光蛋白質―ポリ(2―ビニルピリジン)複合体の調製と機能評価 (2012)
  • Author(s): 沖山直矢、水野稔久、酒井俊介、近藤政晴、出羽毅久、奥淳一、田中俊樹
  • Organizer: 日本化学会第92春期年会(2012)
  • Place of Presentation: 横浜
  • Year and Date: 2012-03-25
• [Presentation] 設計蛋白質を用いたポリチオフェンナノ粒子の調製 (2011)
  • Author(s): 右近卓也、水野稔久、近藤政晴、出羽毅久、田中俊樹、杉安和憲、竹内正之
  • Organizer: 第60回高分子討論会
  • Place of Presentation: 岡山
  • Year and Date: 2011-09-30
• [Presentation] 設計蛋白質を用いたポリチオフェンナノ粒子の調製と機能評価 (2011)
  • Author(s): 右近卓也、水野稔久、出羽毅久、田中俊樹、杉安和憲、竹内正之
  • Organizer: 第5回バイオ関連化学シンポジウム
  • Place of Presentation: つくば
  • Year and Date: 2011-09-12
• [Presentation] タンパク質とPEDOTの複合化による PEDOT/Proteinナノ粒子の調製
  • Author(s): 市来健太郎、水野稔久、近藤政晴、出羽毅久、田中俊樹
  • Organizer: 第62回高分子学会年次大会
  • Place of Presentation: 京都国際会館
• [Presentation] PEDOT/Proteinナノ粒子の調製と機能評価
  • Author(s): 市来健太郎、水野稔久、近藤雅晴、出羽毅久、田中俊樹
  • Organizer: 第23回バイオ高分子シンポジウム
  • Place of Presentation: 東工大大岡山キャンパス
• [Presentation] Facile Preparation of Water-Soluble Poly(3,4-ethylenedioxythiophene) (PEDOT)/Protein Nanoparticle
  • Author(s): Kentaro Ichiki, Takuya Ukon, Masaharu Kondo, Takehisa Dewa, Toshiki Tanaka, Toshihisa Mizuno
  • Organizer: IGER International Symposium on Cell Surface Structures and Functions
  • Place of Presentation: 名古屋大学
• [Presentation] PEDOT/Proteinナノ粒子の調製法の最適化
  • Author(s): 市来健太郎、水野稔久、近藤政晴、出羽毅久、田中俊樹
  • Organizer: 第7回バイオ関連化学シンポジウム
  • Place of Presentation: 名古屋大学
• [Presentation] PEDOT/Protein ナノ粒子の調製と生成機構の検討
  • Author(s): 市来健太郎、水野稔久、近藤政晴、出羽毅久、田中俊樹
  • Organizer: 日本化学会第９４春期年会
  • Place of Presentation: 名古屋大学
• [Presentation] 種々の蛋白質とPEDOTの複合化によるPEDOT/Proteinナノ粒子の調製
  • Author(s): 市来健太郎、水野稔久、近藤政晴、出羽毅久、田中俊樹
  • Organizer: 日本化学会第９３春期年会
  • Place of Presentation: 滋賀
• [Presentation] Formation of planar bilayer membranes on solid supports using peptide-based gemini surfactants
  • Author(s): Katsunari Umezaki, Shunsuke Sakai, Shuhei Koeda, Yasushi Yamamoto, Shinya Ohiwa, Atsushi Ikeda, Takehisa Dewa, Keijiro Taga, Toshiki Tanaka, Toshihisa Mizuno
  • Organizer: International Association of Colloid and Interface Scientists (IACIS) 2012
  • Place of Presentation: Sendai
• [Presentation] ペプチド骨格を含むジェミニ型界面活性剤の新規合成と平面二分子膜形成
  • Author(s): 水野稔久、梅崎勝成、小枝周平、酒井俊亮、山本靖、池田篤志、近藤政晴、多賀圭次郎、出羽毅久、田中俊樹
  • Organizer: 第６１回高分子学会年次大会
  • Place of Presentation: 横浜
• [Presentation] 光化学系I, II (PSI, PSII)を用いたPG-surfactant の膜タンパク質可溶化試薬としての機能評価
  • Author(s): 小枝 周平、水野 稔久、野地 智康、梅崎 勝成、酒井 俊亮、出羽 毅久、田中 俊樹、南後 守
  • Organizer: 第２２回バイオ・高分子シンポジウム
  • Place of Presentation: 東京
• [Presentation] Preparation and characterization of green fluorescent protein (GFP)-poly (2-vinylpyridine) conjugates
  • Author(s): Naoya Okiyama, Eriko Ohta, Jun-ichi Oku, Takehisa Dewa, Toshihisa Mizuno, Toshiki Tanaka
  • Organizer: The 2nd Asian Chemical biology Conference
  • Place of Presentation: Itoman
• [Presentation] Evaluation of photo-induced electron transfer activity of Photosystem I, II (PSI, II) derived from Thermosynechococcus elongatus using peptide gemini surfactants
  • Author(s): Shuhei Koeda, Katsunari Umezaki, Shunsuke Sakai, Tomoyasu Noji, Takehisa Dewa, Toshihisa Mizuno, Toshiki Tanaka
  • Organizer: The 2nd Asian Chemical biology Conference
  • Place of Presentation: Itoman
• [Presentation] 新規ペプチドジェミニ界面活性剤（PG-surfactant）の設計と膜蛋白質研究への応用
  • Author(s): 水野 稔久・小枝 周平・梅崎 勝成・野地 智博・池田 篤志・山本 靖・多賀 圭次郎・田中 俊樹・出羽 毅久・南後 守
  • Organizer: 第６回バイオ関連化学合同シンポジウム
  • Place of Presentation: 札幌
• [Presentation] 分子内架橋したPG-Surfactantの合成と機能評価
  • Author(s): 梅崎勝成・水野稔久*・酒井俊介・近藤政晴・山本靖・出羽毅久・田中俊樹・多賀圭次郎・池田篤志・杉安和憲・竹内正之
  • Organizer: 第６回バイオ関連化学合同シンポジウム
  • Place of Presentation: 札幌
• [Presentation] pH応答性高分子の付加によるGFPナノファイバーの作製
  • Author(s): 沖山直矢・水野稔久*・大田英理子・奥淳一・出羽毅久・田中俊樹・杉安和憲・竹内正之
  • Organizer: 第６１回高分子討論会
  • Place of Presentation: 名古屋
• [Presentation] Synthesis and Characterization of PG-Surfactants, Having Oligo Asp as a Peptide Linker
  • Author(s): Katsunari Umezaki, Toshihisa Mizuno, Shunsuke Sakai, Masaharu Kondo, Yasushi Yamamoto, Takehisa Dewa, Toshiki Tanaka, Atsushi Ikeda, Kasunori Sugiyasu, Masayuki Takeuchi
  • Organizer: The 9th SPSJ International Polymer Conference (IPC2012)
  • Place of Presentation: 神戸
• [Presentation] Evaluation of Photo-Induced Electron-Transfer Activity of Photosystem I, II (PS I, II) Derived from Thermocynechoccus eleongatus Using Peptide Gemini Surfactant
  • Author(s): Shuhei Koeda, Katsunari Umezaki, Shunsuke Sakai, Tomohiro Noji, Takehisa Dewa, Toshihisa Mizuno, Toshki Tanaka, Mamoru Nango, Kazunori Sugiyasu, Masayuki Takeuchi
  • Organizer: The 9th SPSJ International Polymer Conference (IPC2012)
  • Place of Presentation: 神戸
• [Presentation] タンデム化PG-surfactantの膜蛋白質可溶化試薬としての機能評価
  • Author(s): 鈴木智之、小枝周平、水野稔久、野地智康、出羽毅久、田中俊樹、南後守
  • Organizer: 日本化学会第９３春期年会
  • Place of Presentation: 滋賀
• [Presentation] 機能性ペプチドをグラフト化したGFPナノファイバーの調製と機能評価
  • Author(s): 沖山直矢、水野稔久、大田英里子、角野歩、野地智康、出羽毅久、奥淳一、田中俊樹、杉安和憲、竹内正之
  • Organizer: 日本化学会第９３春期年会
  • Place of Presentation: 滋賀
• [Presentation] 機能性分子を導入したPG-surfactantを利用した膜タンパク質の可溶化
  • Author(s): 小枝周平、梅崎勝成、水野稔久、野地智康、出羽毅久、田中俊樹、南後守、杉安和憲、竹内正之
  • Organizer: 日本化学会第９３春期年会
  • Place of Presentation: 滋賀
• [Presentation] 設計蛋白質を用いたポリチオフェンナノ粒子の調製と機能評価
  • Author(s): 右近卓也、水野稔久、出羽毅久、田中俊樹、杉安和憲、竹内正之
  • Organizer: 第５回バイオ関連化学シンポジウム
  • Place of Presentation: つくば
• [Presentation] 設計蛋白質を用いたポリチオフェンナノ粒子の調製
  • Author(s): 右近卓也、水野稔久、近藤政晴、出羽毅久、田中俊樹、杉安和憲、竹内正之
  • Organizer: 第６０回高分子討論会
  • Place of Presentation: 岡山
• [Patent(Industrial Property Rights)] 膜タンパク質ゲル化剤 (2013)
  • Inventor(s): 小枝周平、水野稔久、野地智康、小幡亜希子、出羽毅久、春日敏宏
  • Industrial Property Rights Holder: 名古屋工業大学長
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2013-157755
  • Filing Date: 2013-07-30
• [Patent(Industrial Property Rights)] 膜タンパク質ゲル化剤 (2013)
  • Inventor(s): 小枝周平、水野稔久、野地智康、小幡亜希子、出羽毅久、春日敏宏
  • Industrial Property Rights Holder: 名古屋工業大学学長
  • Industrial Property Rights Type: 特許
  • Filing Date: 2013-07-30
• [Patent(Industrial Property Rights)] 膜タンパク質可溶化剤 (2012)
  • Inventor(s): 小枝周平、梅崎勝成、野地智康、出羽毅久、水野稔久
  • Industrial Property Rights Holder: 名古屋工業大学長
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2012-237380
  • Filing Date: 2012-10-29
• [Patent(Industrial Property Rights)] 膜タンパク質可溶化剤 (2012)
  • Inventor(s): 小枝周平、梅崎勝成、野地智康、出羽毅久、水野稔久
  • Industrial Property Rights Holder: 名古屋工業大学学長
  • Industrial Property Rights Type: 特許
  • Filing Date: 2012-10-29
• [Patent(Industrial Property Rights)] アミノ酸配列含有両親媒性化合物 (2011)
  • Inventor(s): 水野稔久、梅崎勝成、小枝周平
  • Industrial Property Rights Holder: 名古屋工業大学長
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2011-175682
  • Filing Date: 2011-08-11