Studies on interprotein electron transfer using artificial protein complexes

• Project/Area Number: 23750190
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Chemistry related to living body
• Research Institution: Osaka University
• Principal Investigator: NOJIRI Masaki 大阪大学, 理学(系)研究科(研究院), 講師 (20333346)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2011: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
• Keywords: 電子移動反応 / 電子伝達蛋白質 / 蛋白質複合体 / 過渡的複合体 / X線結晶構造解析 / ブルー銅蛋白質 / シトクロムc / 亜硝酸還元酵素 / 電子伝達タンパク質複合体 / 蛋白質電子移動 / 電子移動 / 金属蛋白質 / 電子伝達 / 複合体

Research Abstract

Generally, the redox-partner proteins form a transient complex during the inter-protein electron transfer reaction. It has been known that there are some modulation mechanisms for effective interportein ET due to the conformational changes in the complex structure. However, most of the details for modulation remain unknown, because it is quite difficult to determine the complex structure due to the instability. So, we planed to analyze the structures and biophysical properties for the complexes constructed artificially and the naturally fused proteins. Based on their data, we discuss and propose the unprecedented mechanism for interprotein ET reaction.

Research Products

• [Journal Article] Structural and functional characterization of the Geobacillus copper nitrite reductase : involvement of the unique N-terminal region in the interprotein electron transfer with its redox partner (2014)
  • Author(s): Fukuda, Y., Koteishi, H., Yoneda, R., Tamada, T., Takami, H., Inoue, T., Nojiri, M.
  • Journal Title: Biochim. Biophys. Acta Volume: 1837 Pages: 396-405
  • Peer Reviewed
• [Journal Article] Structural insights into the function of a thermostable copper-containing nitrite reductase (2014)
  • Author(s): Fukuda, Y., Tse, K. M., Lintuluoto, M., Fukunishi, Y., Mizohata, E., Matsumura, H., Takami, H., Nojiri, M., Inoue, T.
  • Journal Title: J. Biochem. Volume: 155 Pages: 123-135
  • Peer Reviewed
• [Journal Article] Structural and functional characterization of the Geobacillus copper nitrite reductase : roles of the unique N-terminal region on the interprotein electron transfer with its redox partner (2014)
  • Author(s): Y Fukuda, H Koteishi, et al.
  • Journal Title: Biochimica et Biephysica Acta Bioenergetics Volume: 1837 Issue: 3 Pages: 396-405
  • DOI: 10.1016/j.bbabio.2014.01.004
  • Peer Reviewed
• [Journal Article] Structural insights into the function of a thermostable copper-containing nitrite reductase (2014)
  • Author(s): Y Fukuda, et al.
  • Journal Title: The Journal of Biochemistry Volume: 155 Issue: 2 Pages: 123-135
  • DOI: 10.1093/jb/mvt107
  • Peer Reviewed
• [Journal Article] The structure of the cytochrome p450cam-putidaredoxin complex determined by paramagnetic NMR spectroscopy and crystallography (2013)
  • Author(s): Hiruma, Y., Hass, M. A., Kikui, Y., Liu, W. M., Ölmez, B., Skinner, S. P., Blok, A., Kloosterman, A., Koteishi, H., Löhr, F., Schwalbe, H., Nojiri, M., Ubbink, M.
  • Journal Title: J. Mol. Biol. Volume: 425 Pages: 4353-4365
  • Peer Reviewed
• [Journal Article] Structural and mechanistic insights into the electron-flow through protein for cytochrome c-tethering copper nitrite reductase (2013)
  • Author(s): Tsuda, A., Ishikawa, R., Koteishi, H., Tange, K., Fukuda, Y., Kobayashi, K., Inoue, T., Nojiri, M.
  • Journal Title: J. Biochem. Volume: 154 Pages: 51-60
  • Peer Reviewed
• [Journal Article] タンパク質分子間電子伝達機構の構造基盤.(総説) (2013)
  • Author(s): 野尻正樹
  • Journal Title: 生化学(日本生化学会) Volume: 85 Pages: 5-12
• [Journal Article] The structure of the cytochrome P450cam-putidaredoxin complex determined by paramagnetic NMR spectroscopy and crystallography. (2013)
  • Author(s): Hiruma, Y., Hass, M.A., Kikui, Y., Liu, W.M., Olmez, B., Skinner, S.P., Blok, A., Kloosterman, A., Koteishi, H., Lohr, F., Schwalbe, H., Nojiri, M., Ubbink, M.
  • Journal Title: J. Mol. Biol. Volume: 425 Issue: 22 Pages: 1127-1133
  • DOI: 10.1016/j.jmb.2013.07.006
  • Peer Reviewed
• [Journal Article] Structural and mechanistic insights into the electron-flow through protein for cytochrome c-tethering copper nitrite reductase (2013)
  • Author(s): 津田愛子, 石川良助, 小手石博康, 丹下幸助, 福田楊太, 小林一雄, 井上豪, 野尻正樹
  • Journal Title: J. Bioche. Volume: 154 Issue: 1 Pages: 51-60
  • DOI: 10.1093/jb/mvt023
  • Peer Reviewed
• [Journal Article] タンパク質分子間電子伝達機構の構造基盤 (2013)
  • Author(s): 野尻正樹
  • Journal Title: 生化学 Volume: 85 Pages: 5-12
  • NAID: 10031146002
• [Journal Article] タンパク質分子間電子移動反応の構造的ならびに機構的洞察 (2012)
  • Author(s): 小手石泰康、野尻正樹
  • Journal Title: 生産と技術(生産技術振興会) Volume: 64 Pages: 76-79
  • NAID: 40019176577
• [Journal Article] タンパク質分子間電子移動反応の構造的ならびに機構的洞察 (2012)
  • Author(s): 小手石泰康、野尻正樹
  • Journal Title: 生産と技術 Volume: 64 Pages: 76-79
  • NAID: 40019176577
• [Journal Article] Cloning, expression, purification, crystallization and preliminary X-ray crystallographic study of GK0767, the copper-containing nitrite reductase from Geobacillus kaustophilus (2011)
  • Author(s): Fukuda, Y., Tamada, T., Takami, H., Suzuki, S., Inoue, T., Nojiri, M.
  • Journal Title: Acta Cryst Volume: F67 Pages: 692-695
  • Peer Reviewed
• [Journal Article] Atomic Description of Inter-protein Electron Transfer for Biological Nitrite Reduction in the Global Nitrogen Cycle (2011)
  • Author(s): Nojiri, M., Suzuki, S.
  • Journal Title: Annual Report of Osaka Univ., Academic Achievement 2009-2010 (10 Papers Selection) Pages: 12-14
• [Journal Article] Atomic Description of Inter-protein Electron Transfer Reaction for Biological Nitrite Reduction in the Global Nitrogen Cycle (2011)
  • Author(s): Nojiri, M.
  • Journal Title: SPring-8 Research Frontiers Volume: 2010 Pages: 30-31
• [Journal Article] 過渡的な生体分子間相互作用を原子レベルで見る (2011)
  • Author(s): 野尻正樹
  • Journal Title: 化学と工業 ("飛翔する若手研究者"欄)(日本化学会) Volume: 10月号 Pages: 790-790
  • NAID: 10029684195
• [Journal Article] Cloning, expression, purification, crystallization and preliminary X-ray crystallographic study of GK0767, the copper-containing nitrite reductase from Geobacillus kaustophilus. (2011)
  • Author(s): Fukuda, Y., Tamada, T., Takami, H., Suzuki, S., Inoue, T., Nojiri, M.
  • Journal Title: Acta Cryst. F Volume: 67 Issue: 6 Pages: 692-695
  • DOI: 10.1107/s1744309111013297
  • Peer Reviewed
• [Presentation] 高度好熱菌由来の銅含有亜硝酸還元酵素の構造と機能 (2013)
  • Author(s): 米田涼平、石原加奈子、永田智大、福田庸太、小手石泰康、井上豪、野尻正樹
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: 横浜パシフィコ
  • Year and Date: 2013-09-11
• [Presentation] 銅含有亜硝酸還元酵素のタイプ1銅部位の原子分解能構造解析と電子伝達活性 (2013)
  • Author(s): 野尻正樹、米田涼平、小手石泰康、福田庸太、平大輔、井上豪
  • Organizer: 第40回生体分子科学討論会
  • Place of Presentation: 大阪大学
  • Year and Date: 2013-06-07
• [Presentation] X-ray diffraction studies on the Protein-Protein Interaction for Interprotein Electron Transfer between Copper Nitrite Reductase and its Redox Partner (2013)
  • Author(s): Nojiri, M.
  • Organizer: ESF-EMBO Symposium "Molecular Perspectives On Protein-Protein Interaction"
  • Place of Presentation: Pultusk, Poland
  • Year and Date: 2013-05-27
• [Presentation] Cytochrome P450camとPutidaredoxinの過渡的電子伝達複合体の結晶構造 (2013)
  • Author(s): 菊井雄基、Yoshitaka Hiruma、小手石泰康、Mathias A.S. Hass、Marcellus Ubbink、野尻正樹
  • Organizer: 平成２５年度日本結晶学会年会
  • Place of Presentation: 熊本大学
• [Presentation] 高度高熱菌由来の銅含有亜硝酸還元酵素の構造と機能 (2013)
  • Author(s): 米田涼平、石原加奈子、永田智大、福田庸太、小手石泰康、井上豪、野尻正樹
  • Organizer: 第８６回日本生化学会大会
  • Place of Presentation: 横浜パシフィコ
• [Presentation] 銅含有亜硝酸還元酵素のタイプ１銅部位の原子分解能構造解析と電子伝達活性 (2013)
  • Author(s): 野尻正樹、米田涼平、小手石泰康、福田庸太、平大輔、井上豪
  • Organizer: 第４０回生体分子科学討論会
  • Place of Presentation: 大阪大学
• [Presentation] X-ray diffraction studies on the protein-protein interaction for interprotein electron transfer between copper nitrite reductase and its redox partner. (2013)
  • Author(s): Nojiri, M.
  • Organizer: ESF-EMBO Res. Conf., On Molecular Perspectives on Protein-Protein Interactions 2013
  • Place of Presentation: Pultusk, Poland
• [Presentation] 生体分子間電子伝達反応における特異的で多様な分子認識機構の構造生物化学 (2012)
  • Author(s): 野尻正樹
  • Organizer: 第92回日本化学会年会「第25回若い世代の特別講演会」
  • Place of Presentation: 慶応義塾大学(講演証受証講演)
  • Year and Date: 2012-03-25
• [Presentation] 生体分子間電子伝達反応における特異的で多様な分子認識機構の構造生物化学（講演証 受証講演） (2012)
  • Author(s): 野尻正樹
  • Organizer: 日本化学会年会（若い世代の特別講演会）(招待講演)
  • Place of Presentation: 慶応義塾大学
• [Presentation] 高分解能電子伝達複合体結晶構造を用いた蛋白質分子間相互作用と電子移動反応過程の解析 (2011)
  • Author(s): 野尻正樹
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都国際会議場 (シンポジウム依頼講演)
  • Year and Date: 2011-09-21
• [Presentation] 高分解能電子伝達複合体構造を用いた蛋白質分子間相互作用と電子移動反応過程の解析 (2011)
  • Author(s): 野尻正樹
  • Organizer: 第49回 日本生物物理学会年会
  • Place of Presentation: 兵庫県立大学 (シンポジウム 依頼講)
  • Year and Date: 2011-09-17
• [Presentation] 高分解能電子伝達複合体構造を用いた蛋白質分子間相互作用と電子移動反応過程の解析 (2011)
  • Author(s): 福田庸太、小手石泰康、井上豪、野尻正樹
  • Organizer: 第11回日本蛋白質科学会年会
  • Place of Presentation: ホテル阪急エキスポパーク
  • Year and Date: 2011-06-08
• [Presentation] 生物学的脱窒過程における蛋白質分子間電子伝達反応の構造基盤（奨励賞 受賞講演） (2011)
  • Author(s): 野尻正樹
  • Organizer: 第８４回日本生化学会大会(招待講演)
  • Place of Presentation: 国立京都国際会議場
• [Presentation] 高分解能電子伝達複合体結晶構造を用いた蛋白質分子間相互作用と電子移動反応過程の解析 (2011)
  • Author(s): 野尻正樹
  • Organizer: 第８４回日本生化学会大会（シンポジウム レドックス反応システムによる細胞機能制御）(招待講演)
  • Place of Presentation: 国立京都国際会議場
• [Presentation] 高分解能電子伝達複合体構造を用いた蛋白質分子間相互作用と電子移動反応過程の解析 (2011)
  • Author(s): 野尻正樹
  • Organizer: 第４９回 日本生物物理学会年会（シンポジウム タンパク質複合体研究の新展開）(招待講演)
  • Place of Presentation: 兵庫県立大学