| Project/Area Number |
23750192
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Chemistry related to living body
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| Research Institution | Ube National College of Technology (2012-2013)
Nara Institute of Science and Technology (2011) |
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Principal Investigator |
HIROHARA Shiho 宇部工業高等専門学校, 物質工学科, 准教授 (70413804)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | X線増感剤 / グルコース連結ポルフィリン金属錯体 / ガン細胞 / 細胞取り込み / 細胞毒性 / 放射線治療 / X線 / Ｘ線増感剤 / 糖連結ポルフィリン金属錯体 / α-トコフェロール / ラット胃癌用細胞株 / ラット正常胃粘膜細胞株 / trans-2置換グルコース連結ポルフィリン / 活性酸素除去剤 / 薬剤取り込み量 / 子宮頚部ガン由来細胞株(HeLa) / 薬剤取り込み |
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| Research Abstract |
Radiotherapy is an excellent treatment for deep-seated cancer. However, repeated irradiation with nearly lethal dose is generally required to kill the tumor, which gives serious strains on patients. In this study, we propose a new radiotherapy which combines X-ray sensitizer with low-dose X-ray irradiation. We synthesized S-, O- and triazol-glucoconjugated porphyrin metal complexes (H2, Zn, Pd, Pt) with all five patterns of glucoconjugation as sensitizers. In addition, the cytotoxicity of porphyrins was evaluated without X-ray irradiation and under X-ray irradiation in normal rat gastric mucosal cells (RGM) and a rat murine RGM gastric carcinoma mucosal cells (RGK). Almost all of porphyrins afforded significantly higher cytotoxicity to RGK cells rather than to RGM cells upon X-ray irradiation. Furthermore, we succeeded in inhibiting the side effects of porphyrin ring, namely photocytotoxicity, by means of the coincubation of alpha-tocopherol with porphyrins.
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