Development of glycoconjugated porphyrin metal complexes for X-ray dynamic therapy
| Project/Area Number |
23750192
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Chemistry related to living body
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| Research Institution | Ube National College of Technology (2012-2013)
Nara Institute of Science and Technology (2011) |
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Principal Investigator |
HIROHARA Shiho 宇部工業高等専門学校, 物質工学科, 准教授 (70413804)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | X線増感剤 / グルコース連結ポルフィリン金属錯体 / ガン細胞 / 細胞取り込み / 細胞毒性 / 放射線治療 / X線 / Ｘ線増感剤 / 糖連結ポルフィリン金属錯体 / α-トコフェロール / ラット胃癌用細胞株 / ラット正常胃粘膜細胞株 / trans-2置換グルコース連結ポルフィリン / 活性酸素除去剤 / 薬剤取り込み量 / 子宮頚部ガン由来細胞株(HeLa) / 薬剤取り込み |
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| Research Abstract |
Radiotherapy is an excellent treatment for deep-seated cancer. However, repeated irradiation with nearly lethal dose is generally required to kill the tumor, which gives serious strains on patients. In this study, we propose a new radiotherapy which combines X-ray sensitizer with low-dose X-ray irradiation. We synthesized S-, O- and triazol-glucoconjugated porphyrin metal complexes (H2, Zn, Pd, Pt) with all five patterns of glucoconjugation as sensitizers. In addition, the cytotoxicity of porphyrins was evaluated without X-ray irradiation and under X-ray irradiation in normal rat gastric mucosal cells (RGM) and a rat murine RGM gastric carcinoma mucosal cells (RGK). Almost all of porphyrins afforded significantly higher cytotoxicity to RGK cells rather than to RGM cells upon X-ray irradiation. Furthermore, we succeeded in inhibiting the side effects of porphyrin ring, namely photocytotoxicity, by means of the coincubation of alpha-tocopherol with porphyrins.
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Report
(4 results)
Research Products
(50 results)
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[Journal Article] Trans-Bisglyco- conjugation is an Efficient and Robust Architecture for PDT Photosensitizers based on 5,10,15,20-Tetrakis(pentafluorophenyl)porphyrin Derivatives2013
Author(s)
S. Hirohara, K. Sharyo, Y. Kawasaki, M. Totani, A. Tomotsuka, R. Funasako, N. Yasui, Y. Hasegawa, J. Yuasa, T. Nakashima, T. Kawai, C. Oka, M. Kawaichi, M. Obata, M. Tanihara
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Journal Title
Bull. Chem. Soc. Jpn.
Volume: 86
Issue: 11
Pages: 1295-1308
DOI
NAID
Related Report
Peer Reviewed
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[Journal Article] Sugar and Heavy Atom Effects of Glycoconjugated Chlorin Palladium Complex on Photocytotoxicity2012
Author(s)
S. Hirohara, Y. Kawasaki, R. Funasako, N. Yasui, M. Totani, H. Alitomo, J. Yuasa, T. Kawai, C. Oka, M. Kawaichi, M. Obata, M. Tanihara
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Journal Title
Bioconjugate Chem.
Volume: Vol.23
Issue: 9
Pages: 1881-1890
DOI
Related Report
Peer Reviewed
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[Presentation] 光線力学療法のための新規物質の開発2012
Author(s)
廣原志保,田村磨聖,高橋成人,中井浩二,金井泰和,渡部浩司,谷原正夫,垣内喜代三,松井裕史,篠原厚,畑澤順
Organizer
第22回日本光線力学学会
Place of Presentation
茨城
Related Report
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