Finding the coenzyme A binding sites and mechanism of enantioselectivity of firefly luciferase catalyzed thioesterification reaction
Project/Area Number |
23750197
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Chemistry related to living body
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Research Institution | University of Hyogo |
Principal Investigator |
KATO Dai-ichiro 兵庫県立大学, 工学(系)研究科(研究院), 助教 (60423901)
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Project Period (FY) |
2011 – 2013
|
Project Status |
Completed (Fiscal Year 2013)
|
Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
|
Keywords | ホタルルシフェラーゼ / チオエステル化 / 立体選択性 / 補酵素A / 結合部位 |
Research Abstract |
Firefly luciferase, which is very famous enzyme for bioluminescence reaction, can also catalyze the enantioselective thioesterification reaction toward 2-arylpropanoic acid. The detail catalytic mechanisms for enantioselectivity and/or a binding site of coenzyme A, however, were not disclosed. Thus in this project, we tried to find the binding site for coenzyme A and tried to explain the mechanism of enantioselectivity toward 2-arylpropanoic acid. Finally we achieved to find some important residues for identifying of substrate chirality.
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Report
(4 results)
Research Products
(50 results)
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[Presentation] Finding the enantioselective thioesterification activity in firefly luciferase2012
Author(s)
D.Kato, Y.Hiraishi, K.Yokoyama, K.Niwa, Y.Ohmiya, M.Takeo, S.Negoro
Organizer
7th CeBiTec Symposium "Bio-integrated Organic Synthesis in Industry : Biocatalytic Breakthroughs, Industrial Processes, Emerging Fields"
Place of Presentation
Center for Interdisciplinary Research (ZiF), Bielefeld University, Germany (P24, ポスター発表)
Related Report
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