| Project/Area Number |
23750202
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Chemistry related to living body
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
UZAWA Takanori 独立行政法人理化学研究所, 伊藤ナノ医工学研究室, 研究員 (60554376)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 核酸 / 両末端の衝突速度 / ダイナミクス / ルテニウム錯体 / 両末端衝突速度 / 発光寿命 |
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| Research Abstract |
Formation of three dimensional structures of a protein and an oligonucleotide require stepwise formation of intramolecular interactions, indicating that intramolecular collision is the most essential process of 3D structure formation. Although the intraolecular collision dynamics of unstructured polypeptides have seen extensively studied, those of oligonucleotides are limited. Thus motivated, in this study I aimed to investigate the base-composition, chain-length and temperature dependences of the end-to-end collision rates for both oligonucleotides of DNA and RNA. I measured the intramolecular collision dynamics using lifetime of a luminophore modified at the end of a oligonucleotide whose the other terminal was modified with a quencher. For a DNA over 20 bases in length, conformational change of polyadenine contributes on the end-to-end collision dynamics at lower temperature. At high temperature, however, this contribution disappears and the dynamics of polyadenine corresponds to the dynamics of polythymine. A similar experiment on RNA did not exhibit clear difference of the luminophore lifetimes between with presence of the quencher and absence of the quencher.
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