Gene carriers containing functional peptides for breast cancer treatment

• Project/Area Number: 23750264
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Polymer/Textile materials
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: NUMATA Keiji 独立行政法人理化学研究所, 酵素研究チーム, チームリーダー (40584529)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: ペプチド / シルク / 遺伝子デリバリー / 癌細胞

Research Abstract

Nano-scale complexes of fusion peptides to contain tumor homing peptides with plasmid DNA (pDNA) are designed as less-cytotoxic and highly target-specific gene carriers. The fusion peptide containing Lyp1 showed significant differences from fusion peptide containing F3 in cytotoxicity to MCF10A cells, and the fusion peptide containing F3 is therefore considered to be the most useful candidate for target delivery into tumorigenic cells. The target specificity of the pDNA complexes to tumorigenic cells was also found to be regulated by specific adsorption process on the cell surface.

Research Products

• [Journal Article] Biopolymer-based Nanoparticles for Drug/Gene Delivery and Tissue Engineering. (2013)
  • Author(s): Sachiko Kaihara Nitta, Keiji Numata
  • Journal Title: International Journal of Molecular Sciences, Special Issue"Bioactive Nanoparticles 2012" Volume: Volume 14, Issue 1 Pages: 1629-1654
  • Peer Reviewed
• [Journal Article] Rapid and Efficient Gene Delivery into Plant Cells Using Designed Peptide Carriers. (2013)
  • Author(s): Manoj Lakshmanan, Yutaka Kodama, Takeshi Yoshizumi, Kumar Sudesh, Keiji Numata
  • Journal Title: Biomacromolecules Volume: Volume 14, Issue 1 Pages: 10-16
  • Peer Reviewed
• [Journal Article] Biopolymer-based Nanoparticles for Drug/Gene Delivery and Tissue Engineering (2013)
  • Author(s): Sachiko Kaihara Nitta
  • Journal Title: International Journal of Molecular Sciences Volume: 14 Issue: 1 Pages: 1629-1654
  • DOI: 10.3390/ijms14011629
  • Peer Reviewed
• [Journal Article] Rapid and Efficient Gene Delivery into Plant Cells Using Designed Peptide Carriers (2013)
  • Author(s): Manoj Lakshmanan
  • Journal Title: Biomacromolecules Volume: 14 Issue: 1 Pages: 10-16
  • DOI: 10.1021/bm301275g
  • Peer Reviewed
• [Journal Article] Silk-Based Nano-Complexes with Tumor Homing Peptides for Tumor-Specific Gene Delivery. (2012)
  • Author(s): Keiji Numata, Aneta J Mieszawska, Laura A. Kvenvold, David L Kaplan
  • Journal Title: Macromolecular Bioscience Volume: Volume 12, Issue 1 Pages: 75-82
  • Peer Reviewed
• [Journal Article] Silk-Based Nano-Complexes with Tumor Homing Peptides for Tumor-Specific Gene Delivery (2012)
  • Author(s): Keiji Numata
  • Journal Title: Macromolecular Bioscience Volume: 12 Issue: 1 Pages: 75-82
  • DOI: 10.1002/mabi.201100274
  • Peer Reviewed
• [Journal Article] Silk-based nanocomplexes with tumor-homing peptides for tumor-specific gene delivery. (2012)
  • Author(s): Numata K, Mieszawska-Czajkowska AJ, Kvenvold LA, Kaplan DL
  • Journal Title: Macromol Biosci. Volume: 1 Pages: 75-82
  • Peer Reviewed
• [Journal Article] Spider Silk-Based Gene Carriers for Tumor Cell-Specific Delivery. (2011)
  • Author(s): Keiji Numata, Michaela R Reagan, Robert H Goldstein, Michael Rosenblatt, David L Kaplan
  • Journal Title: Bioconjugate Chemistry Volume: Volume 22, Issue 8 Pages: 1605-1610
  • Peer Reviewed
• [Presentation] Rapid and efficient gene delivery into plant cells using fusion peptide carrier (2012)
  • Author(s): Keiji Numata, Yutaka Kodama, Takeshi Yoshizumi
  • Organizer: International Polymer Conference
  • Place of Presentation: Kobe, Japan
  • Year and Date: 2012-12-14
• [Presentation] Chemo-enzymatic synthesis of peptide polymers and their application for gene delivery (2012)
  • Author(s): Keiji Numata
  • Organizer: American Chemical Society, Symposium on "Green Polymer Chemistry: Biocatalysis and Biobased Materials"
  • Place of Presentation: USA
  • Year and Date: 2012-08-21
• [Presentation] バイオポリエステルおよびバイオポリアミドの生合成とその応用 (2012)
  • Author(s): 沼田圭司
  • Organizer: エコマテリアル研究会
  • Place of Presentation: 東京
  • Year and Date: 2012-07-06
• [Presentation] Virus-inspired gene delivery systems into plant cells via functional polypeptides (2012)
  • Author(s): Keiji Numata
  • Organizer: Gordon Research Conference on Bioinspired Materials
  • Place of Presentation: USA
  • Year and Date: 2012-06-27
• [Presentation] Gene delivery systems mediated polypeptides into animal and plant cells (2012)
  • Author(s): Keiji Numata
  • Organizer: Gordon Research Conference on Peptides, Chemistry & Biology of
  • Place of Presentation: USA
  • Year and Date: 2012-02-22
• [Presentation] ペプチドポリマーによるソフトマテリアルデザイン (2012)
  • Author(s): 沼田圭司
  • Organizer: 第二回新規材料創成を目指した合成生物学
  • Place of Presentation: 横浜
  • Year and Date: 2012-01-27
• [Presentation] 酵素触媒を用いた新規バイオポリマー合成 (2012)
  • Author(s): 沼田圭司
  • Organizer: 植物科学シンポジウム植物最先端研究への期待
  • Place of Presentation: 品川、コクヨ
• [Presentation] Gene delivery systems mediated polypeptides into animal and plant cells (2012)
  • Author(s): Keiji Numata
  • Organizer: Gordon Research Conference on Peptides, Chemistry & Biology of
  • Place of Presentation: カリフォルニア、米国
• [Presentation] Material design of silk polymers for hydrogel and nanoparticle (2011)
  • Author(s): Keiji Numata
  • Organizer: The 3rd International Conference on Biobased Polymers(招待講演)
  • Place of Presentation: 北京、中国
• [Presentation] Silk hydrogel and nanoparticle as biomaterials (2011)
  • Author(s): Keiji Numata
  • Organizer: Polymers for Advanced Technologies
  • Place of Presentation: Lodz, Poland
• [Presentation] apid and efficient gene delivery into plant cells using fusion peptide carrier
  • Author(s): Keiji Numata
  • Organizer: International Polymer Conference
  • Place of Presentation: 神戸
• [Presentation] Chemo-enzymatic synthesis of peptide polymers and their application for gene delivery
  • Author(s): Keiji Numata
  • Organizer: American Chemical Society, Symposium on "Green Polymer Chemistry: Biocatalysis and Biobased Materials"
  • Place of Presentation: Philadelphia USA
  • Invited
• [Presentation] Virus-inspired gene delivery systems into plant cells via functional polypeptides
  • Author(s): Keiji Numata
  • Organizer: Gordon Research Conference on Bioinspired Materials
  • Place of Presentation: Davidson USA
• [Presentation] 酵素触媒を用いた新規バイオポリマー合成
  • Author(s): 沼田圭司
  • Organizer: 植物科学シンポジウム 植物最先端研究への期待
  • Place of Presentation: 品川
  • Invited
• [Presentation] バイオポリエステルおよびバイオポリアミドの生合成とその応用
  • Author(s): 沼田圭司
  • Organizer: エコマテリアル研究会
  • Place of Presentation: 東京大学
  • Invited
• [Book] Engineering peptide-based carriers for drug and gene delivery. (2014)
  • Author(s): Jo-Ann Chuah, David Kaplan, Keiji Numata
  • Publisher: Engineering in Translational Medicine.Springer, London. (受理済み)
• [Book] Engineering in Translational Medicine (2014)
  • Author(s): Jo-Ann Chuah
  • Publisher: Springer
• [Remarks] 理化学研究所 酵素研究チーム
  • URL: http://www.riken.jp/bmep/teams/enzyme_research/index.html
• [Remarks] Global medical discovery.(2013/03/08)
  • URL: http://globalmedicaldiscovery.com
• [Remarks] Rapid and efficient gene delivery into plant cells using designed Peptide carriers.
  • URL: http://globalmedicaldiscovery.com/key-scientific-articles/rapid-and-efficientgene-delivery-into-plant-cells-using-designed-peptide-carriers/
• [Remarks] Genetically engineered spider silk improve gene therapy, Kurzwelilai News (Aug. 11, 2011)
  • URL: http://www.kurzweilai.net/genetically-engineered-spider-silk-improvegene-therapy
• [Remarks] Genetically Engineered Spider Silk for Gene Therapy, ScienceDaily (Aug. 11, 2011) - Genetically engineered spider silk could help overcome a major barrier to the use of gene therapy in everyday medicine, according to a new study that reported development and successful initial laboratory tests of such a material. It appears in ACS' journal Bioconjugate Chemistry.
  • URL: http://www.sciencedaily.com/releases/2011/08/110810101559.htm