Study on continuous density-gradient centrifugation using microfluidics

• Project/Area Number: 23760150
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Fluid engineering
• Research Institution: Japan Advanced Institute of Science and Technology
• Principal Investigator: UKITA Yoshiaki 北陸先端科学技術大学院大学, マテリアルサイエンス研究科, 助教 (40578100)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2011: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
• Keywords: 細胞分離 / マイクロフルイディクス / 密度勾配遠心法 / 希少細胞 / ディスク型MicroTAS / ２次流れ

Research Abstract

The new device for automated and continuous density gradient centrifugation was developed. Stable mlultilaminar flow was successfully patterned on a microfluidics. The device was applied to continuous mode density-gradient centrifugation to demonstrate separation of micro resin particle and blood cells. As the result, the resin beads were removed from the mixture with performance of 100 % of removal.

Research Products

• [Journal Article] Control of secondary flow in concentrically traveling flow on centrifugal microfluidics (2013)
  • Author(s): Yoshiaki Ukita, Yuzuru Takamura
  • Journal Title: Microfluidics and Nanofluidics Volume: 15 Issue: 6 Pages: 829-837
  • DOI: 10.1007/s10404-013-1194-9
  • Peer Reviewed
• [Presentation] Study on secondary flow behavior in centrifugal microfluidics (2012)
  • Author(s): Yoshiaki Ukita, Takayuki Oguro, Yuzuru Takamura
  • Organizer: JAIST ISEN (International Seminar on Emerging Nanotechnology) 2012
  • Place of Presentation: Ishikawa
  • Year and Date: 2012-03-28
• [Presentation] Formation of stable multilaminar flow on centrifugal microfluidic device and application to density gradient separation (2012)
  • Author(s): Yoshiaki Ukita, Yuzuru Takamura
  • Organizer: International Joint Symposium on Single-Cell Analysis (The 6th International Workshop on Approaches to Single-Cell Analysis & The 8th International Forum on Post-Genome Technologies)
  • Place of Presentation: Kyoto Research Park
• [Presentation] Continuous density gradient centrifugation using a centrifugal microfluidic device (2012)
  • Author(s): Takayuki Oguro, Yoshiaki Ukita, Yuzuru Takamura
  • Organizer: 25th International Microprocesses and Nanotechnology Conference (MNC2012)
  • Place of Presentation: Kobe Meriken Park Oriental Hotel
• [Presentation] Switching of secondary flow behavior on cetrifugal microfluidics (2012)
  • Author(s): Yoshiaki Ukita, Yuzuru Takamura
  • Organizer: The16th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2012)
  • Place of Presentation: OKINAWA
• [Presentation] Visualization of Biological Fluid Behaviour on Spinning Centrifugal Microfluidics using Coaxial Micro-Stroboscope (2012)
  • Author(s): Yoshiaki Ukita, Takayuki Oguro, Masaki Ishizawa, Hiroki Nose, Yuichi Utsumi,Yuzuru Takamura
  • Organizer: 38th International Conferenceon Micro and Nano Engineering (MNE2012)
  • Place of Presentation: Toulouse France
• [Presentation] 遠心送液型マイクロ流体デバイスを用いた血球分離挙動の可視化 (2012)
  • Author(s): 小黒崇之, 浮田芳昭, 高村禅
  • Organizer: 2012年秋季第73回応用物理学会学術講演会
  • Place of Presentation: 愛媛大学・松山大学
• [Presentation] 遠心送液型マイクロフルイディスク内の2次流れ挙動 (2012)
  • Author(s): 浮田芳昭, 小黒崇之, 高村禅
  • Organizer: 2012年春季第59回応用物理学関係連合講演会
  • Place of Presentation: 早稲田大学
• [Presentation] Formation of stable multilaminar flow on centrifugal microfluidic device and application to density gradient separation, (2012)
  • Author(s): Yoshiaki Ukita
  • Organizer: International Joint Symposium on Single-Cell Analysis (The 6th International Workshop on Approaches to Single-Cell Analysis & The 8th International Forum on Post-Genome Technologies),
  • Place of Presentation: 京都
• [Presentation] Continuous density gradient centrifugation using a centrifugal microfluidic device, (2012)
  • Author(s): Takayuki Oguro
  • Organizer: 25th International Microprocesses and Nanotechnology Conference (MNC2012),
  • Place of Presentation: 神戸
• [Presentation] Switching of secondary flow behavior on cetrifugal microfluidics, (2012)
  • Author(s): Yoshiaki Ukita
  • Organizer: The 16th International Conference on Miniaturized Systems for Chemistry and Life sciences (μTAS2012),
  • Place of Presentation: 沖縄
• [Presentation] Visualization of Biological Fluid Behaviour on Spinning Centrifugal Microfluidics using Coaxial Micro-Stroboscope, (2012)
  • Author(s): Yoshiaki Ukita
  • Organizer: 38th International Conference on Micro and Nano Engineering (MNE2012),
  • Place of Presentation: フランス
• [Presentation] 遠心送液型マイクロフルイディスク内の２次流れ挙動 (2012)
  • Author(s): 浮田芳昭、小黒崇之、高村禅
  • Organizer: 2012年春季第59回応用物理学関係連合講演会
  • Place of Presentation: 早稲田大学（東京都）
• [Presentation] Study on secondary flow behavior in centrifugal microfluidics (2012)
  • Author(s): Yoshiaki Ukita, Takayuki Oguro, Yuzuru Takamura
  • Organizer: International Seminar on Emerging Nanotechnology
  • Place of Presentation: 北陸先端科学技術大学院大学（石川県）
• [Presentation] 遠心送液型マイクロ流体デバイス上の層流挙動 (2011)
  • Author(s): 浮田芳昭、小黒崇之、高村禅
  • Organizer: 第24回 化学とマイクロ・ナノシステム研究会
  • Place of Presentation: 大阪府立大学（大阪府）
• [Presentation] 遠心力によるマイクロバイオ単位操作の可視化 (2011)
  • Author(s): 小黒崇之、浮田芳昭、高村禅
  • Organizer: 平成23年度応用物理学会 北陸信越支部学術講演会
  • Place of Presentation: 金沢歌劇座（石川県）
• [Patent(Industrial Property Rights)] 送液方法 (2012)
  • Inventor(s): 浮田芳昭, 小黒崇之, 高村禅
  • Industrial Property Rights Holder: 国立大学法人北陸先端科学技術大学
  • Industrial Property Number: 2012-119476
  • Filing Date: 2012-09-11
• [Patent(Industrial Property Rights)] 送液方法 (2012)
  • Inventor(s): 浮田芳昭、小黒崇之、高村禅
  • Industrial Property Rights Holder: 国立大学法人北陸先端科学技術大学院大学
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2012-199476
  • Filing Date: 2012-09-11