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Anaerobic spores (C. perfringens) as a possible new fecal source tracking indicator of human fecal pollution.

Research Project

Project/Area Number 23760508
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Civil and environmental engineering
Research InstitutionPrefectural University of Hiroshima

Principal Investigator

HASHIMOTO Atsushi  県立広島大学, 生命環境学部, 准教授 (30332068)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords嫌気性芽胞菌 / クリプトスポリジウム / 指標 / ウェルシュ菌 / 下水 / 糞便汚染指標 / 毒素遺伝子
Research Abstract

Clostridium perfringens survive in various aquatic environments than current traditional fecal indicator. Therefore, C. perfringens considered as suitable indicator for fecal pollution. This research described that the new possibility of C. perfringens as a useful source tracking indicator of human fecal pollution using distributions of cpe positive C. perfringens. To evaluate the efficacy of source tracking indicator of C. perfringens, distributions of C. perfringens spores and there toxin type in sewage and livestock fecal samples were analyzed. C. perfringens spores were isolated from sewage, effluents and fecal samples of cattle and pig by multiplex PCR. Almost all isolates were classified type A. From human sewage and effluents, cpe positive C. perfringens were detected at 30%. Whereas only 1 cattle isolate were positive cpe gene from livestock samples. Distributions of cpe positive C. perfringens were important as a potential source tracking indicator for human fecal pollution.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (5 results)

All 2014 2013 2012 2011

All Presentation (5 results)

  • [Presentation] 海域における糞便汚染指標としての嫌気性芽胞菌の有効性2014

    • Author(s)
      土岡宏彰,住吉佑介,原田浩幸,橋本温
    • Organizer
      第48回日本水環境学会年会
    • Place of Presentation
      仙台
    • Related Report
      2013 Final Research Report
  • [Presentation] 海域における糞便汚染指標としての嫌気性芽胞菌の有効性2014

    • Author(s)
      土岡宏章、住吉佑介、原田浩幸、橋本温
    • Organizer
      日本水環境学会年会 仙台2014
    • Place of Presentation
      東北大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] 糞便汚染指標としての嫌気性芽胞菌とその毒素遺伝子による分類2013

    • Author(s)
      住吉佑介,金川慎之輔,橋本温
    • Organizer
      第47回日本水環境学会年会
    • Place of Presentation
      大阪
    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Presentation] ヒト・豚由来排水から分離した嫌気性芽胞菌の毒素遺伝子による分類2012

    • Author(s)
      住吉佑介,橋本温
    • Organizer
      第63回全国水道研究発表会
    • Place of Presentation
      松江
    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Presentation] 各種水環境から分離した嫌気性芽胞菌の分類2011

    • Author(s)
      住吉佑介,七種健一郎,河村俊輔,橋本温
    • Organizer
      第11回環境技術学会研究発表大会
    • Place of Presentation
      大阪
    • Related Report
      2013 Final Research Report 2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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