Kinetic analysis of cellulosic biomass degradation based on an electrochemical measurement combined with calorimetry
| Project/Area Number |
23760746
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Shinshu University |
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Principal Investigator |
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| Research Collaborator |
CRUYS-BAGGER Nicolaj ロスキレ大学, 博士研究員
BORCH Kim ノボザイム, 研究員
WESTH Peter ロスキレ大学, 教授
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| Project Period (FY) |
2011-04-28 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | バイオマス / バイオセンサー / カロリメトリー |
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| Outline of Final Research Achievements |
The kinetics of the enzymatic hydrolysis of cellulosic biomass was studied by real-time monitoring with amperometic biosensor and calorimetry. The response time of the biosensor was improved to follow a rapid change in reaction rates at a pre-steady state of cellobiohydrolase reaction. By the kinetic analysis, it was concluded that the rapid decrease in the rate of cellobiohydrolase reaction at a pre-steady state was derived from the slow dissociation of the enzyme from a cellulose chain.
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Report
(5 results)
Research Products
(24 results)
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[Journal Article] A pyranose dehydrogenase-based biosensor for kinetic analysis ofenzymatic hydrolysis of cellulose by cellulases2014
Author(s)
cellulasesNicolaj Cruys-Bagger, Silke Flindt Badino, Radina Tokin, Mark Gontsarik, Samin Fathalinejad, Kenneth Jensen, Miguel Duarte Toscano, Trine Holst Sørensen, Kim Borch, Hirosuke Tatsumi, Priit Väljamäe, Peter Westh
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Journal Title
Enzyme and Microbial Technology
Volume: 58-59
Pages: 68-74
DOI
Related Report
Peer Reviewed
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