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Kinetic analysis of cellulosic biomass degradation based on an electrochemical measurement combined with calorimetry

Research Project

Project/Area Number 23760746
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Biofunction/Bioprocess
Research InstitutionShinshu University

Principal Investigator

TATSUMI Hirosuke  信州大学, 学術研究院理学系, 准教授 (60336609)

Research Collaborator CRUYS-BAGGER Nicolaj  ロスキレ大学, 博士研究員
BORCH Kim  ノボザイム, 研究員
WESTH Peter  ロスキレ大学, 教授
Project Period (FY) 2011-04-28 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywordsバイオマス / バイオセンサー / カロリメトリー
Outline of Final Research Achievements

The kinetics of the enzymatic hydrolysis of cellulosic biomass was studied by real-time monitoring with amperometic biosensor and calorimetry. The response time of the biosensor was improved to follow a rapid change in reaction rates at a pre-steady state of cellobiohydrolase reaction. By the kinetic analysis, it was concluded that the rapid decrease in the rate of cellobiohydrolase reaction at a pre-steady state was derived from the slow dissociation of the enzyme from a cellulose chain.

Report

(5 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (24 results)

All 2014 2013 2012 2011

All Journal Article (14 results) (of which Peer Reviewed: 13 results,  Acknowledgement Compliant: 2 results) Presentation (10 results) (of which Invited: 3 results)

  • [Journal Article] Development of dropping carbon fluid electrodes for polarography2014

    • Author(s)
      Hirosuke Tatsumi, Shogo Tanaka
    • Journal Title

      Electrochimica Acta

      Volume: 135 Pages: 255-259

    • DOI

      10.1016/j.electacta.2014.04.146

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Sensitive electrochemical measurement of hydroxyl radical generation induced by the xanthine-xanthine oxidase system2014

    • Author(s)
      Hirosuke Tatsumi, Yui Tsuchiya, Koichi Sakamoto
    • Journal Title

      Analytical Biochemistry

      Volume: 467 Pages: 22-27

    • DOI

      10.1016/j.ab.2014.08.028

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] A graphene screen-printed carbon electrode for real-time measurements2014

    • Author(s)
      Nicolaj Cruys-Bagger, Hirosuke Tatsumi, Kim Borch, Peter Westh
    • Journal Title

      Analytical Biochemistry

      Volume: 447 Pages: 162-168

    • DOI

      10.1016/j.ab.2013.11.024

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] A pyranose dehydrogenase-based biosensor for kinetic analysis ofenzymatic hydrolysis of cellulose by cellulases2014

    • Author(s)
      cellulasesNicolaj Cruys-Bagger, Silke Flindt Badino, Radina Tokin, Mark Gontsarik, Samin Fathalinejad, Kenneth Jensen, Miguel Duarte Toscano, Trine Holst Sørensen, Kim Borch, Hirosuke Tatsumi, Priit Väljamäe, Peter Westh
    • Journal Title

      Enzyme and Microbial Technology

      Volume: 58-59 Pages: 68-74

    • DOI

      10.1016/j.enzmictec.2014.03.002

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] バイオセンサを用いる糖質バイオマス分解過程の解析2013

    • Author(s)
      巽 広輔
    • Journal Title

      Chemical Sensors

      Volume: 29 Pages: 9-14

    • NAID

      40019672231

    • Related Report
      2013 Research-status Report
  • [Journal Article] Transient Kinetics and Rate-Limiting Steps for the Processive2013

    • Author(s)
      Nicolaj Cruys-Bagger, Hirosuke Tatsumi, Guilin Robin Ren, Kim Borch, and Peter Westh
    • Journal Title

      Biochemistry

      Volume: 52 Issue: 49 Pages: 8938-8948

    • DOI

      10.1021/bi401210n

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Origin of Initial Burst in Activity for Trichoderma reesei endo-Glucanases Hydrolyzing Insoluble Cellulose2012

    • Author(s)
      L. Murphy, N. Cruys-Bagger, H. D. Damgaard, M. J. Baumann, S. N. Olsen, K. Borch, S. F. Lassen, M. Sweeney, H. Tatsumi, P. Westh
    • Journal Title

      J. Biol. Chem.

      Volume: 287 Issue: 2 Pages: 1252-1260

    • DOI

      10.1074/jbc.m111.276485

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Pre-steady-state Kinetics for Hydrolysis of Insoluble Cellulose by Cellobiohydrolase Cel7A2012

    • Author(s)
      N. Cruys-Bagger, J. Elmerdahl, E. Praestgaard, H. Tatsumi, N. Spodsberg, K. Borch, P. Westh
    • Journal Title

      J. Biol. Chem.

      Volume: 287 Issue: 22 Pages: 18451-18458

    • DOI

      10.1074/jbc.m111.334946

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Polarography with a dropping carbon electrode2012

    • Author(s)
      H. Tatsumi, M. Shiba
    • Journal Title

      Electrochem. Commun.

      Volume: 20 Pages: 160-162

    • DOI

      10.1016/j.elecom.2012.04.021

    • NAID

      120007114286

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] An Amperometric Enzyme Biosensor for Real-Time Measurements of Cellobiohydrolase Activity on Insoluble Cellulose2012

    • Author(s)
      N. Cruys-Bagger, G. Ren, H. Tatsumi, M. J. Baumann, N. Spodsberg, H. D. Andersen, L. Gorton, K. Borch, P. Westh
    • Journal Title

      Biotechnol. Bioeng.

      Volume: 109 Issue: 12 Pages: 3199-3204

    • DOI

      10.1002/bit.24593

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Kinetic Studies on Enzymatic Hydrolysis of Polysaccharides by Amperometric Biosensors2012

    • Author(s)
      H. Tatsumi
    • Journal Title

      Rev. Polarogr.

      Volume: 58 Pages: 75-82

    • NAID

      10031120425

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Origin of Initial Burst in Activity for Trichoderma reesei endo-Glucanases Hydrolyzing Insoluble Cellulose2012

    • Author(s)
      L. Murphy, N. Cruys-Bagger, H. D. Damgaard, M. J. Baumann, S. N. Olsen, K. Borch, S. F. Lassen, M. Sweeney, H. Tatsumi, P. Westh
    • Journal Title

      Journal of Biological Chemistry

      Volume: 287 Pages: 1252-1260

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Pre-steady State Kinetics for the Hydrolysis of Insoluble Cellulose by Cellobiohydrolase Cel7A2012

    • Author(s)
      N. Cruys-Bagger, J. Elmerdahl, E. Praestgaard, H. Tatsumi, N. Spodsberg, K. Borch, P. Westh
    • Journal Title

      Journal of Biological Chemistry

      Volume: 印刷中

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Sensitive Electrochemical Detection of the Hydroxyl Radical Using Enzyme-catalyzed Redox Cycling2011

    • Author(s)
      H. Tatsumi, N. Osaku
    • Journal Title

      Analytical Sciences

      Volume: 27 Pages: 1065-1067

    • NAID

      10029881397

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] 液状炭素電極を用いるポーラログラフィー.電極攪拌装置の検討.2014

    • Author(s)
      清家成彬、巽広輔
    • Organizer
      第60回ポーラログラフィーおよび電気分析化学討論会
    • Place of Presentation
      京都工芸繊維大学(京都市)
    • Year and Date
      2014-11-15 – 2014-11-16
    • Related Report
      2014 Annual Research Report
  • [Presentation] 液状炭素電極を用いるポーラログラフィー2014

    • Author(s)
      巽広輔
    • Organizer
      2014年度第2回関西電気化学研究会
    • Place of Presentation
      京都大学桂キャンパス(京都市)
    • Year and Date
      2014-10-18
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Sensitive Electrochemical Measurement of Hydroxyl Radical Generation Induced by the Xanthine-Xanthine Oxidase System2014

    • Author(s)
      Hirosuke Tatsumi, Yui Tsuchiya, Koichi Sakamoto
    • Organizer
      1st Asian Symposium on Analytical Sciences
    • Place of Presentation
      広島大学東広島キャンパス(東広島市)
    • Year and Date
      2014-09-17 – 2014-09-19
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Sensitive Electrochemical Measurement of Hydroxyl Radical Generation Induced by the Xanthine-Xanthine Oxidase System2014

    • Author(s)
      Hirosuke Tatsumi, Yui Tsuchiya, Koichi Sakamoto
    • Organizer
      65th Annual Meeting of the International Society of Electrochemistry
    • Place of Presentation
      EPFL、ローザンヌ(スイス)
    • Year and Date
      2014-08-31 – 2014-09-05
    • Related Report
      2014 Annual Research Report
  • [Presentation] 酵素触媒レドックスサイクリングを用いるOHラジカルの高感度検出2013

    • Author(s)
      巽 広輔
    • Organizer
      第44回中部化学関係学協会支部連合秋季大会
    • Place of Presentation
      静岡大学浜松キャンパス
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] 滴下炭素電極を用いるポーラログラフィー2013

    • Author(s)
      田中翔悟、巽 広輔
    • Organizer
      第59回ポーラログラフィーおよび電気分析化学討論会
    • Place of Presentation
      石垣市民会館
    • Related Report
      2013 Research-status Report
  • [Presentation] CARBON PASTE ELECTRODE MODIFIED WITH FERRIC HYDROXIDE FOR THE SENSITIVE DETECTION OF PARACETAMOL2012

    • Author(s)
      Irdhawati, Hirosuke Tatsumi
    • Organizer
      Regional Electrochemistry Meeting of South-East Asia 2012 (REMSEA 2012)
    • Place of Presentation
      EDSA Shangri-La Hotel, Manila, Philippines
    • Related Report
      2012 Research-status Report
  • [Presentation] 滴下炭素電極を用いるポーラログラフィー2012

    • Author(s)
      巽 広輔、田中翔悟
    • Organizer
      日本分析化学会第61年会
    • Place of Presentation
      金沢大学
    • Related Report
      2012 Research-status Report
  • [Presentation] Pre-steady state kinetics of cellobiohydrolase I on insoluble cellulose2011

    • Author(s)
      P. Westh, N. Cruys-Bagger, E. Praestgaard, H. Tatsumi, N. Spodsberg, K. Borch
    • Organizer
      Gordon Research Conferences (Cellulosomes, Cellulases & Other Carbohydrate Modifying Enzymes)
    • Place of Presentation
      Stonehill College, Easton, MA (USA)
    • Related Report
      2011 Research-status Report
  • [Presentation] Application of enzyme-modified electrodes based on cellobiose dehydrogenase in cellulose research2011

    • Author(s)
      N. Cruys-Bagger, H. Tatsumi, K. Borch, P. Westh
    • Organizer
      Annual Meeting of Danish Electrochemical Society
    • Place of Presentation
      University of Southern Denmark, Odense (Denmark)
    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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