Kinetic analysis of cellulosic biomass degradation based on an electrochemical measurement combined with calorimetry

• Project/Area Number: 23760746
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biofunction/Bioprocess
• Research Institution: Shinshu University
• Principal Investigator: TATSUMI Hirosuke 信州大学, 学術研究院理学系, 准教授 (60336609)
• Research Collaborator: CRUYS-BAGGER Nicolaj ロスキレ大学, 博士研究員 ; BORCH Kim ノボザイム, 研究員 ; WESTH Peter ロスキレ大学, 教授
• Project Period (FY): 2011-04-28 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: バイオマス / バイオセンサー / カロリメトリー

Outline of Final Research Achievements

The kinetics of the enzymatic hydrolysis of cellulosic biomass was studied by real-time monitoring with amperometic biosensor and calorimetry. The response time of the biosensor was improved to follow a rapid change in reaction rates at a pre-steady state of cellobiohydrolase reaction. By the kinetic analysis, it was concluded that the rapid decrease in the rate of cellobiohydrolase reaction at a pre-steady state was derived from the slow dissociation of the enzyme from a cellulose chain.

Research Products

• [Journal Article] Development of dropping carbon fluid electrodes for polarography (2014)
  • Author(s): Hirosuke Tatsumi, Shogo Tanaka
  • Journal Title: Electrochimica Acta Volume: 135 Pages: 255-259
  • DOI: 10.1016/j.electacta.2014.04.146
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Sensitive electrochemical measurement of hydroxyl radical generation induced by the xanthine-xanthine oxidase system (2014)
  • Author(s): Hirosuke Tatsumi, Yui Tsuchiya, Koichi Sakamoto
  • Journal Title: Analytical Biochemistry Volume: 467 Pages: 22-27
  • DOI: 10.1016/j.ab.2014.08.028
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] A graphene screen-printed carbon electrode for real-time measurements (2014)
  • Author(s): Nicolaj Cruys-Bagger, Hirosuke Tatsumi, Kim Borch, Peter Westh
  • Journal Title: Analytical Biochemistry Volume: 447 Pages: 162-168
  • DOI: 10.1016/j.ab.2013.11.024
  • Peer Reviewed
• [Journal Article] A pyranose dehydrogenase-based biosensor for kinetic analysis ofenzymatic hydrolysis of cellulose by cellulases (2014)
  • Author(s): cellulasesNicolaj Cruys-Bagger, Silke Flindt Badino, Radina Tokin, Mark Gontsarik, Samin Fathalinejad, Kenneth Jensen, Miguel Duarte Toscano, Trine Holst Sørensen, Kim Borch, Hirosuke Tatsumi, Priit Väljamäe, Peter Westh
  • Journal Title: Enzyme and Microbial Technology Volume: 58-59 Pages: 68-74
  • DOI: 10.1016/j.enzmictec.2014.03.002
  • Peer Reviewed
• [Journal Article] バイオセンサを用いる糖質バイオマス分解過程の解析 (2013)
  • Author(s): 巽 広輔
  • Journal Title: Chemical Sensors Volume: 29 Pages: 9-14
  • NAID: 40019672231
• [Journal Article] Transient Kinetics and Rate-Limiting Steps for the Processive (2013)
  • Author(s): Nicolaj Cruys-Bagger, Hirosuke Tatsumi, Guilin Robin Ren, Kim Borch, and Peter Westh
  • Journal Title: Biochemistry Volume: 52 Issue: 49 Pages: 8938-8948
  • DOI: 10.1021/bi401210n
  • Peer Reviewed
• [Journal Article] Origin of Initial Burst in Activity for Trichoderma reesei endo-Glucanases Hydrolyzing Insoluble Cellulose (2012)
  • Author(s): L. Murphy, N. Cruys-Bagger, H. D. Damgaard, M. J. Baumann, S. N. Olsen, K. Borch, S. F. Lassen, M. Sweeney, H. Tatsumi, P. Westh
  • Journal Title: J. Biol. Chem. Volume: 287 Issue: 2 Pages: 1252-1260
  • DOI: 10.1074/jbc.m111.276485
  • Peer Reviewed
• [Journal Article] Pre-steady-state Kinetics for Hydrolysis of Insoluble Cellulose by Cellobiohydrolase Cel7A (2012)
  • Author(s): N. Cruys-Bagger, J. Elmerdahl, E. Praestgaard, H. Tatsumi, N. Spodsberg, K. Borch, P. Westh
  • Journal Title: J. Biol. Chem. Volume: 287 Issue: 22 Pages: 18451-18458
  • DOI: 10.1074/jbc.m111.334946
  • Peer Reviewed
• [Journal Article] Polarography with a dropping carbon electrode (2012)
  • Author(s): H. Tatsumi, M. Shiba
  • Journal Title: Electrochem. Commun. Volume: 20 Pages: 160-162
  • DOI: 10.1016/j.elecom.2012.04.021
  • NAID: 120007114286
  • Peer Reviewed
• [Journal Article] An Amperometric Enzyme Biosensor for Real-Time Measurements of Cellobiohydrolase Activity on Insoluble Cellulose (2012)
  • Author(s): N. Cruys-Bagger, G. Ren, H. Tatsumi, M. J. Baumann, N. Spodsberg, H. D. Andersen, L. Gorton, K. Borch, P. Westh
  • Journal Title: Biotechnol. Bioeng. Volume: 109 Issue: 12 Pages: 3199-3204
  • DOI: 10.1002/bit.24593
  • Peer Reviewed
• [Journal Article] Kinetic Studies on Enzymatic Hydrolysis of Polysaccharides by Amperometric Biosensors (2012)
  • Author(s): H. Tatsumi
  • Journal Title: Rev. Polarogr. Volume: 58 Pages: 75-82
  • NAID: 10031120425
  • Peer Reviewed
• [Journal Article] Origin of Initial Burst in Activity for Trichoderma reesei endo-Glucanases Hydrolyzing Insoluble Cellulose (2012)
  • Author(s): L. Murphy, N. Cruys-Bagger, H. D. Damgaard, M. J. Baumann, S. N. Olsen, K. Borch, S. F. Lassen, M. Sweeney, H. Tatsumi, P. Westh
  • Journal Title: Journal of Biological Chemistry Volume: 287 Pages: 1252-1260
  • Peer Reviewed
• [Journal Article] Pre-steady State Kinetics for the Hydrolysis of Insoluble Cellulose by Cellobiohydrolase Cel7A (2012)
  • Author(s): N. Cruys-Bagger, J. Elmerdahl, E. Praestgaard, H. Tatsumi, N. Spodsberg, K. Borch, P. Westh
  • Journal Title: Journal of Biological Chemistry Volume: 印刷中
  • Peer Reviewed
• [Journal Article] Sensitive Electrochemical Detection of the Hydroxyl Radical Using Enzyme-catalyzed Redox Cycling (2011)
  • Author(s): H. Tatsumi, N. Osaku
  • Journal Title: Analytical Sciences Volume: 27 Pages: 1065-1067
  • NAID: 10029881397
  • Peer Reviewed
• [Presentation] 液状炭素電極を用いるポーラログラフィー．電極攪拌装置の検討． (2014)
  • Author(s): 清家成彬、巽広輔
  • Organizer: 第60回ポーラログラフィーおよび電気分析化学討論会
  • Place of Presentation: 京都工芸繊維大学（京都市）
  • Year and Date: 2014-11-15 – 2014-11-16
• [Presentation] 液状炭素電極を用いるポーラログラフィー (2014)
  • Author(s): 巽広輔
  • Organizer: 2014年度第2回関西電気化学研究会
  • Place of Presentation: 京都大学桂キャンパス（京都市）
  • Year and Date: 2014-10-18
  • Invited
• [Presentation] Sensitive Electrochemical Measurement of Hydroxyl Radical Generation Induced by the Xanthine-Xanthine Oxidase System (2014)
  • Author(s): Hirosuke Tatsumi, Yui Tsuchiya, Koichi Sakamoto
  • Organizer: 1st Asian Symposium on Analytical Sciences
  • Place of Presentation: 広島大学東広島キャンパス（東広島市）
  • Year and Date: 2014-09-17 – 2014-09-19
  • Invited
• [Presentation] Sensitive Electrochemical Measurement of Hydroxyl Radical Generation Induced by the Xanthine-Xanthine Oxidase System (2014)
  • Author(s): Hirosuke Tatsumi, Yui Tsuchiya, Koichi Sakamoto
  • Organizer: 65th Annual Meeting of the International Society of Electrochemistry
  • Place of Presentation: EPFL、ローザンヌ（スイス）
  • Year and Date: 2014-08-31 – 2014-09-05
• [Presentation] 酵素触媒レドックスサイクリングを用いるOHラジカルの高感度検出 (2013)
  • Author(s): 巽 広輔
  • Organizer: 第44回中部化学関係学協会支部連合秋季大会
  • Place of Presentation: 静岡大学浜松キャンパス
  • Invited
• [Presentation] 滴下炭素電極を用いるポーラログラフィー (2013)
  • Author(s): 田中翔悟、巽 広輔
  • Organizer: 第59回ポーラログラフィーおよび電気分析化学討論会
  • Place of Presentation: 石垣市民会館
• [Presentation] CARBON PASTE ELECTRODE MODIFIED WITH FERRIC HYDROXIDE FOR THE SENSITIVE DETECTION OF PARACETAMOL (2012)
  • Author(s): Irdhawati, Hirosuke Tatsumi
  • Organizer: Regional Electrochemistry Meeting of South-East Asia 2012 (REMSEA 2012)
  • Place of Presentation: EDSA Shangri-La Hotel, Manila, Philippines
• [Presentation] 滴下炭素電極を用いるポーラログラフィー (2012)
  • Author(s): 巽 広輔、田中翔悟
  • Organizer: 日本分析化学会第61年会
  • Place of Presentation: 金沢大学
• [Presentation] Pre-steady state kinetics of cellobiohydrolase I on insoluble cellulose (2011)
  • Author(s): P. Westh, N. Cruys-Bagger, E. Praestgaard, H. Tatsumi, N. Spodsberg, K. Borch
  • Organizer: Gordon Research Conferences (Cellulosomes, Cellulases & Other Carbohydrate Modifying Enzymes)
  • Place of Presentation: Stonehill College, Easton, MA (USA)
• [Presentation] Application of enzyme-modified electrodes based on cellobiose dehydrogenase in cellulose research (2011)
  • Author(s): N. Cruys-Bagger, H. Tatsumi, K. Borch, P. Westh
  • Organizer: Annual Meeting of Danish Electrochemical Society
  • Place of Presentation: University of Southern Denmark, Odense (Denmark)