| Project/Area Number |
23760748
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
|
| Project Status |
Completed (Fiscal Year 2012)
|
| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2011: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
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| Keywords | ゼブラフィッシュ / 抗体測定 / ネイティブフォーム抗原 / 膜タンパク質 / 抗体 / 立体構造 / GPCR |
|---|
| Research Abstract |
Using zebrafish, Danio rerio, embryo which is known for next generation recombinant expression host, a recombinant human phospholipase A2 receptor (hPLA2R) protein, a kind of membrane associate protein, with native-form conformation was successfully generated. Moreover, we demonstrated that anti-hPLA2R IgG which recognize aepitope structure from patient with membranous nephropathy were successfully measured with this recombinant hPLA2R protein as antigen.
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