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Enzymatic oxidation reactions using clustered liposomes that specifically response to shear flow and temperature change

Research Project

Project/Area Number 23760751
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Biofunction/Bioprocess
Research InstitutionYamaguchi University

Principal Investigator

YOSHIMOTO Makoto  山口大学, 大学院・医学系研究科, 准教授 (80322246)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywordsリポソーム / 酵素反応 / バイオリアクター / ナノバイオ / せん断流
Research Abstract

The membrane properties of closed phospholipid bilayers (liposomes) could be altered by relatively small changes in temperature and shear stress generated by the flow and mixing conditions in bioreactors. These membrane properties of liposomes were regulated based on their composition, size and clustering characteristics. Based on the results obtained, the catalytic oxidation reaction systems, where the stability and reactivity of enzymes could be widely regulated, were developed using various types of enzyme-containing liposomes suspended in a bioreactor.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (29 results)

All 2013 2012 2011 Other

All Journal Article (12 results) (of which Peer Reviewed: 12 results) Presentation (17 results)

  • [Journal Article] Membrane permeability and stability of liposomes suspended in shear flow2013

    • Author(s)
      T. Natsume and M. Yoshimoto
    • Journal Title

      J. Dispersion Sci. Technol

      Volume: (in press) Issue: 11 Pages: 1557-1562

    • DOI

      10.1080/01932691.2012.752712

    • Year and Date
      2013-11-02
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Preparation of liposome-coupled NADH and evaluation of its affinity toward formate dehydrogenase based on deactivation kinetics of the enzyme.2013

    • Author(s)
      M. Yoshimoto, N. Kunihiro, N. Tsubomura and M. Nakayama
    • Journal Title

      Coll. Surf. B

      Volume: 109 Pages: 40-44

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Oxidation of glucose in gas-liquid flow catalyzed by glucose oxidase-containing liposomes with different acyl chain properties2013

    • Author(s)
      M. Inoue and M. Yoshimoto
    • Journal Title

      J. Chem. Eng. Jpn

      Volume: 46 Pages: 302-306

    • NAID

      10031187994

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Oligolamellar vesicles for covalent immobilization and stabilization of D-amino acid oxidase2013

    • Author(s)
      M. Yoshimoto, M. Yamasaki, M. Okamoto, H. Umakoshi and R. Kuboi
    • Journal Title

      Enzyme Microb. Technol

      Volume: 52 Pages: 13-19

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Preparation of liposome-coupled NADH and evaluation of its affinity toward formate dehydrogenase based on deactivation kinetics of the enzyme2013

    • Author(s)
      M. Yoshimoto
    • Journal Title

      Coll. Surf. B

      Volume: 109 Pages: 40-44

    • DOI

      10.1016/j.colsurfb.2013.03.023

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Oxidation of Glucose in Gas–Liquid Flow Catalyzed by Glucose Oxidase-Containing Liposomes with Different Acyl Chain Properties2013

    • Author(s)
      M. Inoue
    • Journal Title

      JOURNAL OF CHEMICAL ENGINEERING OF JAPAN

      Volume: 46 Issue: 4 Pages: 302-306

    • DOI

      10.1252/jcej.12we290

    • NAID

      10031187994

    • ISSN
      0021-9592, 1881-1299
    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Oligolamellar vesicles for covalent immobilization and stabilization of D-amino acid oxidase2013

    • Author(s)
      M. Yoshimoto
    • Journal Title

      Enzyme Microb. Technol.

      Volume: 52 Issue: 1 Pages: 13-19

    • DOI

      10.1016/j.enzmictec.2012.09.001

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] リポソーム懸濁系の物質移動を伴う酵素反応2012

    • Author(s)
      吉本誠
    • Journal Title

      膜

      Volume: 37 Pages: 270-275

    • NAID

      10031131136

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Reactivity and stability of catalase-bound liposomes containing glucose oxidase in an airlift bubble column2012

    • Author(s)
      M. Yoshimoto, M. Inoue and N. Takaki
    • Journal Title

      J. Chem. Eng. Jpn

      Volume: 45 Pages: 645-650

    • NAID

      130001858768

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Reactivity and Stability of Catalase-Bound Liposomes Containing Glucose Oxidase in an Airlift Bubble Column2012

    • Author(s)
      Makoto Yoshimoto, Masahiro Inoue and Noriyuki Takaki
    • Journal Title

      JOURNAL OF CHEMICAL ENGINEERING OF JAPAN

      Volume: 45 Issue: 9 Pages: 645-650

    • DOI

      10.1252/jcej.12we051

    • NAID

      130001858768

    • ISSN
      0021-9592, 1881-1299
    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Effects of Bubble Interactions in Circulating Liquid Flow on Liquid Phase Mass Transfer Coefficient in an External Loop Airlift Bubble Column2012

    • Author(s)
      Makoto Yoshimoto, Keiji Furumoto and Katsumi Nakao
    • Journal Title

      JOURNAL OF CHEMICAL ENGINEERING OF JAPAN

      Volume: 45 Issue: 9 Pages: 661-665

    • DOI

      10.1252/jcej.12we048

    • NAID

      130001858767

    • ISSN
      0021-9592, 1881-1299
    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Effects of Bubble Interactions on Liquid Phase Mass Transfer Coefficients in Three Types of Bubble Columns2012

    • Author(s)
      Katsumi Nakao, Keiji Furumoto and Makoto Yoshimoto
    • Journal Title

      JOURNAL OF CHEMICAL ENGINEERING OF JAPAN

      Volume: 45 Issue: 9 Pages: 655-660

    • DOI

      10.1252/jcej.12we047

    • NAID

      130001858766

    • ISSN
      0021-9592, 1881-1299
    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] Permeability and stability of phospholipid bilayer membranes under fluid shear stress in a cone-and-plate geometry2012

    • Author(s)
      T. Natsume and M. Yoshimoto
    • Organizer
      The 18th Symp. Young Asian Biochem. Engineer's Community (YABEC2012)
    • Place of Presentation
      徳島,徳島大学
    • Year and Date
      2012-10-27
    • Related Report
      2012 Final Research Report
  • [Presentation] 液体せん断流中における脂質膜透過性とリポソーム系酸化酵素反応2012

    • Author(s)
      吉本誠,夏目友誉,井上正大
    • Organizer
      分離技術会年会2012
    • Place of Presentation
      吹田,関西大学
    • Year and Date
      2012-06-02
    • Related Report
      2012 Final Research Report
  • [Presentation] 脂質二分子膜の安定性と透過性に及ぼすせん断ストレス条件と懸濁リポソーム径の影響2012

    • Author(s)
      夏目友誉,堤賢太郎,吉本誠
    • Organizer
      化学工学会第77 年会
    • Place of Presentation
      東京,工学院大学
    • Year and Date
      2012-03-15
    • Related Report
      2012 Final Research Report
  • [Presentation] 脂質二分子膜の安定性と透過性に及ぼすせん断ストレス条件と懸濁リポソーム径の影響2012

    • Author(s)
      夏目友誉,堤賢太郎,吉本誠
    • Organizer
      化学工学会第77年会
    • Place of Presentation
      工学院大学(東京都)
    • Related Report
      2011 Research-status Report
  • [Presentation] 脂質二分子膜の透過性に及ぼすせん断流と温度の影響2011

    • Author(s)
      夏目友誉,堤賢太郎,吉本誠
    • Organizer
      第4回化学工学 3 支部合同福井大会
    • Place of Presentation
      福井,ホテルフジタ福井
    • Year and Date
      2011-12-08
    • Related Report
      2012 Final Research Report
  • [Presentation] Activity and structural change of catalase-bound liposomes containing glucose oxidase suspended in an external loop airlift bubble column2011

    • Author(s)
      M. Yoshimoto, M. Inoue and N. Takaki
    • Organizer
      1st Int. Symp. Multiscale Multiphase Process Eng.(MMPE)
    • Place of Presentation
      金沢,金沢市文化ホール
    • Year and Date
      2011-10-05
    • Related Report
      2012 Final Research Report
  • [Presentation] リポソーム系複合酵素のグルコース酸化活性と安定性に及ぼす気泡塔内気液流動の影響2011

    • Author(s)
      井上正大,高木規行,吉本誠
    • Organizer
      化学工学会第43回秋季大会
    • Place of Presentation
      名古屋工業大学(名古屋市)
    • Related Report
      2011 Research-status Report
  • [Presentation] 高温条件を利用するリポソーム膜に共有結合させた補酵素と脱水素酵素間相互作用の評価2011

    • Author(s)
      坪村直樹,國廣信貴,吉本誠
    • Organizer
      化学工学会第43回秋季大会
    • Place of Presentation
      名古屋工業大学(名古屋市)
    • Related Report
      2011 Research-status Report
  • [Presentation] Activity and structural change of catalse-bound liposomes containing glucose oxidase suspended in an external loop airlift bubble column2011

    • Author(s)
      Makoto Yoshimoto, Masahiro Inoue and Noriyuki Takaki
    • Organizer
      1st International Symposium on Multiscale Multiphase Process Engineering
    • Place of Presentation
      Kanazawa Culture Hall (Kanazawa city)
    • Related Report
      2011 Research-status Report
  • [Presentation] Control of complexation between dehydrogenase and cofactors for thermostable liposomal biocatalysts2011

    • Author(s)
      Makoto Yoshimoto, Naoki Tsubomura, Nobutaka Kunihiro, Takayuki Yamashita and Satoshi Kinoshita
    • Organizer
      9th International Conference on Separation Science and Technology
    • Place of Presentation
      Jeju Grand Hotel (Jeju, Korea)
    • Related Report
      2011 Research-status Report
  • [Presentation] 脂質二分子膜の透過性に及ぼすせん断流と温度の影響2011

    • Author(s)
      夏目友誉,堤賢太郎,吉本誠
    • Organizer
      第4回化学工学3支部合同福井大会
    • Place of Presentation
      ホテルフジタ福井(福井市)
    • Related Report
      2011 Research-status Report
  • [Presentation] リポソーム系アミノ酸オキシダーゼ反応によるD-アミノ酸の脂質膜透過性の評価

    • Author(s)
      吉本誠
    • Organizer
      化学工学会高松大会
    • Place of Presentation
      高松市(サンポートホール高松)
    • Related Report
      2012 Annual Research Report
  • [Presentation] リポソーム内セルラーゼの安定性と不溶性セルロース糖化活性に及ぼすイオン液体の影響

    • Author(s)
      吉本誠
    • Organizer
      化学工学会高松大会
    • Place of Presentation
      高松市(サンポートホール高松)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Permeability and stability of phospholipid bilayer membranes under fluid shear stress in a cone-and-plate geometry

    • Author(s)
      M Yoshimoto
    • Organizer
      The 18th Symp. Young Asian Biochem. Engineer’s Community (YABEC2012)
    • Place of Presentation
      徳島市(徳島大学)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 液体せん断流中における脂質膜透過性とリポソーム系酸化酵素反応

    • Author(s)
      吉本誠
    • Organizer
      分離技術会年会2012
    • Place of Presentation
      吹田市(関西大学)
    • Related Report
      2012 Annual Research Report
  • [Presentation] オリゴラメラ構造をもつリポソームによるD-アミノ酸オキシダーゼ活性の安定化

    • Author(s)
      吉本誠
    • Organizer
      分離技術会年会2012
    • Place of Presentation
      吹田市(関西大学)
    • Related Report
      2012 Annual Research Report
  • [Presentation] リポソーム内水相を用いる安定な炭酸カルシウム微粒子の調製

    • Author(s)
      吉本誠
    • Organizer
      分離技術会年会2012
    • Place of Presentation
      吹田市(関西大学)
    • Related Report
      2012 Annual Research Report

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Published: 2011-08-05   Modified: 2019-07-29  

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