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Chemical biology approach to ganglioside-induced amyloidosis

Research Project

Project/Area Number 23760758
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Biofunction/Bioprocess
Research InstitutionThe Institute of Physical and Chemical Research

Principal Investigator

SAKONO MASAFUMI  独立行政法人理化学研究所, 伊藤細胞制御化学研究室, 客員研究員 (50391959)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywordsガングリオシド / アミロイド / アミロイドベータ / 糖鎖生物学 / 脂質ラフト / 糖脂質 / クリック反応 / リポソーム
Research Abstract

Amyloid beta which is causative agent of Alzheimer's disease is widely known to form aggregate nucleation by binding with ganglioside GM1, and this nucleation is suggested to enhance aggregation of amyloid beta in vivo. In this study, the synthesis of non-native form ganglioside based on GM1 structure was investigated to clarify the interaction between ganglioside and amyloid beta. We examined synthesis pathway of GM1-type glycan which possess reactive site by click chemistry, and obtained optimal synthesis method.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (25 results)

All 2013 2012 2011

All Journal Article (11 results) (of which Peer Reviewed: 11 results) Presentation (14 results)

  • [Journal Article] Formation of non-toxic Aβfibrils by small heat shock protein under heat-stress conditions2013

    • Author(s)
      Masafumi Sakono, Arata Utsumi, Tamotsu Zako, Tetsuya Abe, Masafumi Yohda and Mizuo Maeda
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 430 Issue: 4 Pages: 1259-1264

    • DOI

      10.1016/j.bbrc.2012.12.059

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Human prefoldin inhibits Aβfibrillation and contributes to formation of non-toxic Aβaggregates2013

    • Author(s)
      K.M. Sörgjerd, T. Zako, M. Sakono, P.C. Stirling, M.R. Leroux, T. Saito, P. Nilsson, M. Sekimoto, T.C.Saido, M. Maeda
    • Journal Title

      Biochemistry

      Volume: 52 Issue: 20 Pages: 3532-3542

    • DOI

      10.1021/bi301705c

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Naked-eye Detection of Amyloid Aggregates Using Gold Nanoparticles Modified with Amyloid Beta Antibody2012

    • Author(s)
      Masafumi Sakono
    • Journal Title

      Analytical Sciences

      Volume: 28 Issue: 1 Pages: 73

    • DOI

      10.2116/analsci.28.73

    • NAID

      130004827182

    • ISSN
      0910-6340, 1348-2246
    • Related Report
      2013 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Amyloid oligomer detection by immobilized molecular chaperone2012

    • Author(s)
      Sakono M. Zako T, Yohda M, Maeda M.
    • Journal Title

      Biochemical Engineering Journal

      Volume: 61 Pages: 28-33

    • DOI

      10.1016/j.bej.2011.12.005

    • Related Report
      2013 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Display of Functionally Active PHB Depolymerase on Escherichia coli Cell Surface2012

    • Author(s)
      Tomohiro Hiraishi, Koichi Yamashita, Masafumi Sakono, Jun Nakanishi, Tan Liu Tzea, Kumar Sudesh, Hideki Abe, and Mizuo Maeda
    • Journal Title

      Macromolecular Bioscience

      Volume: 12 Issue: 2 Pages: 218-224

    • DOI

      10.1002/mabi.201100273

    • Related Report
      2013 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Cell interaction study of amyloid using luminescent conjugated polythiophene : Implication that amyloid cytotoxicity is correlated with prolonged cellular binding2012

    • Author(s)
      Tamotsu Zako
    • Journal Title

      ChemBioChem

      Volume: (in press) Issue: 3 Pages: 358-363

    • DOI

      10.1002/cbic.201100467

    • Related Report
      2013 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Chemical synthesis of intentionally misfolded homogeneous glycoprotein: a unique approach for the study of glycoprotein quality control.2012

    • Author(s)
      Masayuki Izumi, Yutaka Makimura, Simone Dedola, Akira Seko, Akiko Kanamori, Masafumi Sakono, Yukishige Ito, and Yasuhiro Kajihara
    • Journal Title

      Journal of the American Chemical Society

      Volume: 134 Issue: 17 Pages: 7238-7241

    • DOI

      10.1021/ja3013177

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Biophysical properties of UDP-glucose:glycoprotein glucosyltransferase, a folding sensor enzyme in the ER, delineated by synthetic probes.2012

    • Author(s)
      Masafumi Sakono, Akira Seko, Yoichi Takeda, Masakazu Hachisu, and Yukishige Ito
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 426 Issue: 4 Pages: 504-510

    • DOI

      10.1016/j.bbrc.2012.08.112

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Alpha casein micelles show not only molecular chaperone-like aggregation inhibition properties but also protein refolding activity from the denatured state2011

    • Author(s)
      Masafumi Sakono, Konomi Motomura, Tatsuo Maruyama, Noriho Kamiya, Masahiro Goto
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 404 Issue: 1 Pages: 494-497

    • DOI

      10.1016/j.bbrc.2010.12.009

    • Related Report
      2013 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Application of two morphologically different fibrillar and filamentous insulin amyloids as a biomaterial for cell culture surfaces2011

    • Author(s)
      Masafumi Sakono
    • Journal Title

      Reactive Functional Polymers

      Volume: 71 Issue: 3 Pages: 324-328

    • DOI

      10.1016/j.reactfunctpolym.2010.10.012

    • Related Report
      2013 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Immobilized insulin amyloid enhances cell adhesion and proliferation due to interaction with fibronectin2011

    • Author(s)
      Masafumi Sakono, Shigenori Akiyama, Tamotsu Zako, Shujiro Sakaki, Tomonori Waku, Naoki Tanaka, Mizuo Maeda
    • Journal Title

      Chemistry Letters

      Volume: 40 Issue: 3 Pages: 315-317

    • DOI

      10.1246/cl.2011.315

    • NAID

      130004426166

    • Related Report
      2013 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Presentation] Synthesis of Erythropoietin bearing high-mannose type oligosaccharide2013

    • Author(s)
      Tatsuo Kiuchi, Yutaka Makimura, Ryo Okamoto, Masayuki Izumi, Akira Seko, Masafumi Sakono, Akiko Kanamori, Yukishige Ito, Yasuhiro Kajihara
    • Organizer
      The 3rd Austria/Japan Seminar on Comparative and Developmental Glycobiology
    • Place of Presentation
      Wako
    • Year and Date
      2013-07-01
    • Related Report
      2013 Final Research Report
  • [Presentation] Analysis of UGGT by using misfolded glycoprotein mimics2013

    • Author(s)
      Masakazu Hachisu, Akira Seko, Shusaku Daikoku, Yoichi Takeda, Akihiko Koizumi, Kohki Fujikawa, Masafumi Sakono, Yukishige Ito
    • Organizer
      The 3rd Austria/Japan Seminar on Comparative and Developmental Glycobiology
    • Place of Presentation
      Wako
    • Year and Date
      2013-07-01
    • Related Report
      2013 Final Research Report
  • [Presentation] 合成基質を用いた糖タンパク質品質管理機構の解明2013

    • Author(s)
      迫野昌文、瀬古玲、武田陽一、八須匡和、小泉晶彦、藤川絋樹、伊藤幸成
    • Organizer
      化学工学会第78年会
    • Place of Presentation
      大阪
    • Year and Date
      2013-03-25
    • Related Report
      2013 Final Research Report
  • [Presentation] 合成糖鎖基質を用いたカルネキシンおよびカルメギンのシャペロン活性の比較2013

    • Author(s)
      迫野昌文、瀬古玲、武田陽一、八須匡和、小泉晶彦、藤川紘樹、伊藤幸成
    • Organizer
      農芸化学会2013年度大会
    • Place of Presentation
      仙台
    • Related Report
      2013 Final Research Report
  • [Presentation] カルネキシンおよびカルメギンのレクチン様分子シャペロン活性の比較2012

    • Author(s)
      迫野昌文、瀬古玲、武田陽一、八須匡和、小泉晶彦、藤川紘樹、伊藤幸成
    • Organizer
      GlycoTOKYO2012
    • Place of Presentation
      東京
    • Year and Date
      2012-11-17
    • Related Report
      2013 Final Research Report
  • [Presentation] カルネキシンおよびカルメギンのレクチン様分子シャペロン活性の相同性に関する検討2012

    • Author(s)
      迫野昌文、瀬古玲、武田陽一、八須匡和、小泉晶彦、藤川紘樹、伊藤幸成
    • Organizer
      第31回日本糖質学会年会
    • Place of Presentation
      鹿児島
    • Year and Date
      2012-09-17
    • Related Report
      2013 Final Research Report
  • [Presentation] 糖基質との相互作用に伴う"フォールディングセンサー"UDP-Glc : Glycoprotein Glucosyltransferase(UGGT)の構造変化に関する検討2012

    • Author(s)
      迫野昌文、武田陽一、八須匡和、瀬古玲、伊藤幸成
    • Organizer
      農芸化学会2012年度大会
    • Place of Presentation
      京都
    • Year and Date
      2012-03-23
    • Related Report
      2013 Final Research Report
  • [Presentation] 分子シャペロンによるアミロイドベータオリゴマーの検出2012

    • Author(s)
      迫野昌文、座古保、養王田正文、前田瑞夫
    • Organizer
      化学工学会第77年会
    • Place of Presentation
      東京
    • Year and Date
      2012-03-15
    • Related Report
      2013 Final Research Report
  • [Presentation] 分子シャペロンによるアミロイドベータオリゴマーの検出2012

    • Author(s)
      迫野昌文,座古保, 養王田正文, 前田瑞夫
    • Organizer
      化学工学会第77年会
    • Place of Presentation
      新宿
    • Related Report
      2011 Research-status Report
  • [Presentation] 糖基質との相互作用に伴う"フォールディングセンサー" UDP-Glc:Glycoprotein Glucosyltransferase(UGGT)の構造変化に関する検討2012

    • Author(s)
      迫野昌文、武田陽一、八須匡和、瀬古玲、伊藤幸成
    • Organizer
      日本農芸化学会2012年度大会
    • Place of Presentation
      京都
    • Related Report
      2011 Research-status Report
  • [Presentation] Conformational Change of Folding Sensor Enzyme UDP-Glc : Glycoprotein Glucosyltransferase (UGGT) by Interaction with Carbohydrate Substrates2011

    • Author(s)
      Masafumi Sakono, Yoichi Takeda, Masakazu Hachisu, Akira Seko, Yukishige Ito
    • Organizer
      The 3rd Asian Communication for Glycobiology and Glycotechnology
    • Place of Presentation
      Shnghai
    • Year and Date
      2011-11-27
    • Related Report
      2013 Final Research Report
  • [Presentation] "フォールディングセンサー"UDP-Glc : Glycoprotein Glucosyltransferase(UGGT)の基質認識に伴う構造変化に関する検討2011

    • Author(s)
      迫野昌文、武田陽一、八須匡和、瀬古玲、伊藤幸成
    • Organizer
      GlycoTOKYO2011
    • Place of Presentation
      東京
    • Year and Date
      2011-11-09
    • Related Report
      2013 Final Research Report
  • [Presentation] 低毒性アミロイド様インスリン凝集体の構造特性2011

    • Author(s)
      迫野昌文、小林隆宏、座古保、Lindgren Mikael、Nilsson Peter、Hammarstrom Per、前田瑞夫
    • Organizer
      化学工学会第76年会
    • Place of Presentation
      東京
    • Year and Date
      2011-03-22
    • Related Report
      2013 Final Research Report
  • [Presentation] Conformational Change of Folding Sensor Enzyme UDP-Glc:Glycoprotein Glucosyltransferase (UGGT) by Interaction with Carbohydrate Substrates2011

    • Author(s)
      Masafumi Sakono, Yoichi Takeda, Masakazu Hachisu, Akira Seko and Yukishige Ito
    • Organizer
      3rd ACGG conference
    • Place of Presentation
      中国 上海
    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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