A new application of compound specific nitrogen isotope ratios analysis of amino acids on feeding ecology study of wildlife

• Project/Area Number: 23770030
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Ecology/Environment
• Research Institution: Forestry and Forest Products Research Institute
• Principal Investigator: NAKASHITA Rumiko 独立行政法人森林総合研究所, 野生動物研究領域, 主任研究員 (00457839)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 野生動物 / 食性 / 安定同位体比 / アミノ酸 / 窒素安定同位体比 / 食性履歴 / 血漿 / 代謝 / ツキノワグマ

Research Abstract

I analyzed the compound specific nitrogen isotope ratios (d15NAA) of amino acids to reconstruct feeding habits of captive Asiatic black bears (food known) and free-ranging black bears and brown bears (food unknown). The results determined by d15Nbulk as a conventional method and by d15NAA method strongly suggested that the d15NAA method revealed more precise feeding habit of animals than the d15Nbulk method. The d15NAA method is a reliable and useful tool for reconstructing feeding habit of wildlife.

Research Products

• [Journal Article] 長野県塩尻市における閉鎖牛舎での捕獲ツキノワグマの家畜飼料依存度 (2014)
  • Author(s): 中下留美子,林秀剛,岸元良輔,鈴木彌生子,瀧井暁子,泉山茂之
  • Journal Title: 信州大学農学部AFC報告 Volume: 12 Pages: 85-90
  • NAID: 120007105506
  • Peer Reviewed
• [Journal Article] 長野県塩尻市における閉鎖牛舎での捕獲ツキノワグマの家畜飼料依存度 (2014)
  • Author(s): 中下留美子、林秀剛、岸元良輔、鈴木彌生子、瀧井暁子、泉山茂之
  • Journal Title: 信州大学農学部AFC 報告 Volume: 12 Pages: 85-90
  • NAID: 120007105506
  • Peer Reviewed
• [Journal Article] 捕まったツキノワグマは何を食べていた?-ツキノワグマの体毛に記録された食性履歴を生元素の同位体比でよみとる- (2013)
  • Author(s): 中下留美子
  • Journal Title: 菌蕈 Volume: 59 Pages: 9-15
• [Journal Article] 森の研究わたしのテーマ第32回「体毛から読み解くツキノワグマの食性履歴」 (2013)
  • Author(s): 中下留美子
  • Journal Title: グリーンパワー Volume: 417 Pages: 12-12
• [Journal Article] 捕まったクマは何を食べていた？‐ツキノワグマの体毛に記録された食性履歴を生元素の同位体比で読みとる- (2013)
  • Author(s): 中下留美子
  • Journal Title: 菌蕈 Volume: 59 Pages: 9-15
  • NAID: 40019695933
• [Journal Article] 森の研究 わたしのテーマ第32回「体毛から読み解くツキノワグマの食性履歴」 (2013)
  • Author(s): 中下留美子
  • Journal Title: グリーンパワー Volume: 417 Pages: 12-12
• [Journal Article] Characteristics of stable isotope signature of diet in tissues of captive Japanese macaques as revealed by controlled feeding (2013)
  • Author(s): Rumiko Nakashita, Yuzuru Hamada, Eishi Hirasaki, Juri Suzuki, Toru Oi
  • Journal Title: Primates Volume: ― Issue: 3 Pages: 271-281
  • DOI: 10.1007/s10329-013-0346-6
  • NAID: 10031185560
  • Peer Reviewed
• [Journal Article] 安定生元素同位体比分析によるツキノワグマの食性解析の方法と被害分析の事例(総説) (2013)
  • Author(s): 中下留美子
  • Journal Title: 森林防疫 Volume: 62 Pages: 13-17
  • Peer Reviewed
• [Journal Article] 牛舎周辺におけるツキノワグマ捕獲個体の家畜飼料依存. (2013)
  • Author(s): 中下留美子・岸元良輔・鈴木彌生子・瀧井暁子・林秀剛・泉山茂之
  • Journal Title: 長野県自然保護研究所研究報告 Volume: 9 Pages: 17-22
  • Peer Reviewed
• [Journal Article] 長野市街地ツキノワグマ出没個体は人里に依存していたか？―安定同位体比分析による食性解析. (2013)
  • Author(s): 泉山茂之・中下留美子・岸元良輔・鈴木彌生子・瀧井暁子・林秀剛
  • Journal Title: 信州大学農学部AFC報告 Volume: 11
  • NAID: 120007105499
  • Peer Reviewed
• [Journal Article] 山ノ内町におけるツキノワグマ人身事故の検証 (2012)
  • Author(s): 泉山茂之・岸元良輔・中下留美子・鈴木彌生子・後藤光章・林秀剛
  • Journal Title: 信州大学農学部AFC報告 Volume: 10 Pages: 133-138
  • NAID: 120007105473
  • Peer Reviewed
• [Journal Article] 塩尻市における牛舎周辺の捕獲ツキノワグマ(Ursus thibetanus)の食性解析 (2012)
  • Author(s): 泉山茂之・中下留美子・鈴木彌生子・岸元良輔・瀧井暁子・林秀剛
  • Journal Title: 信州大学農学部AFC報告 Volume: 10 Pages: 139-143
  • NAID: 120007105474
  • Peer Reviewed
• [Journal Article] Ecological application of compound- specific stable nitrogen isotope analysis of amino acids?A case study for captive and wild bears (2011)
  • Author(s): R. Nakashita, Y. Suzuki, F. Akamatsu, Y. I. Naito, M. Hashimoto, T. Tsubota
  • Journal Title: Researches in Organic Geochemistry Volume: 27 Pages: 73-79
  • Peer Reviewed
• [Journal Article] Ecological application of compound-specific … (2011)
  • Author(s): Rumiko Nakashita, Y. Suzuki, F. Akamatsu, …
  • Journal Title: Researches in Organic Geochemistry Volume: 27 Pages: 73-79
  • Peer Reviewed
• [Presentation] Feeding habits of brown bears (Ursus arctos) of the Southern Kurils islands revealed by multi-stable isotope analysis (2013)
  • Author(s): R. Nakashita, Y. Suzuki, K. Kobayashi, T. Ito, H. Nakamura, Y. Masuda, K. Kawai, A. Loguntsev, N. Ohtaishi, Y.Sato
  • Organizer: International Association for Ecology
  • Place of Presentation: London, UK.
  • Year and Date: 2013-08-22
• [Presentation] 生元素安定同位体比解析からみた国後島および択捉島のヒグマの食生態 (2013)
  • Author(s): R. Nakashita, Y. Suzuki, K. Kobayashi, T. Ito, H. Nakamura, Y. Masuda, K. Kawai, A. Loguntsev, N. Ohtaishi, Y.Sato
  • Organizer: Japan Geoscience Union Meeting 2013
  • Place of Presentation: 千葉県幕張メッセ.
  • Year and Date: 2013-05-23
• [Presentation] Feeding habits of brown bears (Ursus arctos) of the Southern Kurils islands revealed by multi-stable isotope analysis. (2013)
  • Author(s): Rumiko Nakashita, Yaeko Suzuki, Kyoko Kobayashi, Tetsuji Ito, Hidetsugu Nakamura, Yasushi Masuda, A Loguntsev, Noriyuki Ohtaishi, Yoshikazu Sato
  • Organizer: International Association for Ecology 2013
  • Place of Presentation: London,UK
• [Presentation] 炭素・窒素・硫黄安定同位体比解析からみた国後島ヒグマの食生態. (2013)
  • Author(s): 中下留美子・鈴木彌生子・小林喬子・伊藤哲治・中村秀次・増田泰・Andrey Longuntsev・大泰司紀之・佐藤喜和
  • Organizer: 日本生態学会第60回大会
  • Place of Presentation: 静岡県コンベンションアーツセンター(静岡県)
• [Presentation] Estimation of the trophic position based on stable nitrogen isotopic composition of amino acids?Evaluation of hibernation effect for Asiatic black bears (2012)
  • Author(s): R. Nakashita, Y. Suzuki, M. Shimozuru, Y. Chikaraishi, N. Ohkouchi, T. Tsubota
  • Organizer: International Conference on Bear Research and Management
  • Place of Presentation: Deli, India
  • Year and Date: 2012-11-26
• [Presentation] Feeding ecology of brown bears of the Southern Kurils revealed by stable isotope analysis (2012)
  • Author(s): R. Nakashita, Y. Suzuki, K. Kobayashi, T. Ito, H. Nakamura, Y. Masuda, K. Kawai, A. Loguntsev, N. Ohtaishi, Y.Sato
  • Organizer: The 8th International Conference on Applications of Stable Isotope Techniques to Ecological Studies
  • Place of Presentation: Brest, France
  • Year and Date: 2012-08-22
• [Presentation] 生元素安定同位体比分析を用いて国後島白いヒグマの生態を探る (2012)
  • Author(s): 中下留美子,小林喬子,鈴木彌生子,伊藤哲治,中村秀次,増田泰,河合久仁子,Andrey Loguntsev,大泰司紀之,佐藤喜和
  • Organizer: 第72回分析化学討論会
  • Place of Presentation: 鹿児島大学
  • Year and Date: 2012-05-20
• [Presentation] Estimation of the trophic position based on stable nitrogen isotopic composition of amino acids - Evaluation of hibernation effect for Asiatic black bears. (2012)
  • Author(s): Nakashita R, Suzuki Y, Shimozuru M, Chikaraishi Y, Ohkouchi N, Tsubota T
  • Organizer: International Conference on Bear Research and Management,
  • Place of Presentation: India Habitat Centre (Deli, India)
• [Presentation] Feeding ecology of brown bears of the Southern Kurils revealed by stable isotope analysis. (2012)
  • Author(s): Nakashita R, Suzuki Y, Kobayashi K, Ito T, Nakamura H, Masuda Y, Kawai K, Loguntsev A, Ohtaishi N, Sato Y
  • Organizer: The 8th International Conference on Applications of Stable Isotope Techniques to Ecological Studies
  • Place of Presentation: Quartz Conference Center (Brest, France)
• [Presentation] LA-ICPMSイメージング法による野生動物の食性解析の可能性―ヒグマ（Ursus arctos）をケーススタディとして―． (2012)
  • Author(s): 中下留美子・大石昌弘・鈴木彌生子・伊藤哲治・小林喬子・中村秀次・増田泰・河合久仁子・Andrey Loguntsev・大泰司紀之・佐藤喜和
  • Organizer: 日本分析化学会第61年会
  • Place of Presentation: 金沢大学(石川県)
• [Presentation] 生元素安定同位体比分析を用いて国後島白いヒグマの生態を探る． (2012)
  • Author(s): 中下留美子・小林喬子・鈴木彌生子・伊藤哲治・中村秀次・増田泰・河合久仁子・Andrey Loguntsev・大泰司紀之・佐藤喜和
  • Organizer: 第72回分析化学討論会
  • Place of Presentation: 鹿児島大学(鹿児島県)
• [Presentation] アミノ酸窒素安定同位体比によるツキノワグマの食性解析 (2011)
  • Author(s): 中下留美子,鈴木彌生子,佐藤美穂,坪田敏男
  • Organizer: 日本哺乳類学会2011年度大会
  • Place of Presentation: 宮崎市
• [Presentation] Fluctuation in the serum C, N, and O stable isotop (2011)
  • Author(s): Nakashita R, Suzuki Y , Sato M, Shimozuru M, ･･･
  • Organizer: 20th International Conference on Bear Research …
  • Place of Presentation: The Westin Ottawa（オタワ)
• [Presentation] 体毛から読みとるツキノワグマの食性履歴 (2011)
  • Author(s): 中下留美子・鈴木彌生子・林秀剛・泉山茂之
  • Organizer: 日本分析化学会 第五回表示・起源分析技術研究懇談会
  • Place of Presentation: さいたま市文化センター（さいたま市）
• [Presentation] アミノ酸窒素安定同位体比によるツキノワグマの食性解析 (2011)
  • Author(s): 中下留美子・鈴木彌生子・佐藤美穂・坪田敏男
  • Organizer: 日本哺乳類学会2011年度大会
  • Place of Presentation: 宮崎市民プラザ（宮崎市）
• [Presentation] 肉牛飼育場に出没したツキノワグマの軽元素安定同位体比 (2011)
  • Author(s): 中下留美子・鈴木彌生子・林秀剛・泉山茂之
  • Organizer: 第17回野生生物保護学会(北海道)大会
  • Place of Presentation: 東京農業大学オホーツクキャンパス（網走）
• [Presentation] 生元素安定同位体比解析からみた国後島および択捉島のヒグマの食生態
  • Author(s): Rumiko Nakashita, Yaeko Suzuki, Kyoko Kobayashi, Tetsuji Ito, Hidetsugu Nakamura, Yasushi Masuda, A Loguntsev, Noriyuki Ohtaishi, Yoshikazu Sato
  • Organizer: Japan Geoscience Union Meeting 2013
  • Place of Presentation: 千葉県幕張メッセ